Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms
tag-Targeted Protein Degrader (tTPD) systems are powerful tools for preclinical target validation. Here the authors extend the tTPD platform by developing NanoTACs that degrade NanoLuc tagged substrates and benchmark each tTPD system using an interchangeable tag reporter system.
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-29670-1 |
| _version_ | 1817990938152140800 |
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| author | Christoph Grohmann Charlene M. Magtoto Joel R. Walker Ngee Kiat Chua Anna Gabrielyan Mary Hall Simon A. Cobbold Stephen Mieruszynski Martin Brzozowski Daniel S. Simpson Hao Dong Bridget Dorizzi Annette V. Jacobsen Emma Morrish Natasha Silke James M. Murphy Joan K. Heath Andrea Testa Chiara Maniaci Alessio Ciulli Guillaume Lessene John Silke Rebecca Feltham |
| author_facet | Christoph Grohmann Charlene M. Magtoto Joel R. Walker Ngee Kiat Chua Anna Gabrielyan Mary Hall Simon A. Cobbold Stephen Mieruszynski Martin Brzozowski Daniel S. Simpson Hao Dong Bridget Dorizzi Annette V. Jacobsen Emma Morrish Natasha Silke James M. Murphy Joan K. Heath Andrea Testa Chiara Maniaci Alessio Ciulli Guillaume Lessene John Silke Rebecca Feltham |
| author_sort | Christoph Grohmann |
| collection | DOAJ |
| description | tag-Targeted Protein Degrader (tTPD) systems are powerful tools for preclinical target validation. Here the authors extend the tTPD platform by developing NanoTACs that degrade NanoLuc tagged substrates and benchmark each tTPD system using an interchangeable tag reporter system. |
| first_indexed | 2024-04-14T01:05:46Z |
| format | Article |
| id | doaj.art-cbe3c5d3eff94f2bbdf917175c5d3791 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-14T01:05:46Z |
| publishDate | 2022-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-cbe3c5d3eff94f2bbdf917175c5d37912022-12-22T02:21:15ZengNature PortfolioNature Communications2041-17232022-04-0113111310.1038/s41467-022-29670-1Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platformsChristoph Grohmann0Charlene M. Magtoto1Joel R. Walker2Ngee Kiat Chua3Anna Gabrielyan4Mary Hall5Simon A. Cobbold6Stephen Mieruszynski7Martin Brzozowski8Daniel S. Simpson9Hao Dong10Bridget Dorizzi11Annette V. Jacobsen12Emma Morrish13Natasha Silke14James M. Murphy15Joan K. Heath16Andrea Testa17Chiara Maniaci18Alessio Ciulli19Guillaume Lessene20John Silke21Rebecca Feltham22The Walter and Eliza Hall Institute for Medical ResearchThe Walter and Eliza Hall Institute for Medical ResearchPromega Biosciences LLCThe Walter and Eliza Hall Institute for Medical ResearchThe Walter and Eliza Hall Institute for Medical ResearchPromega Biosciences LLCThe Walter and Eliza Hall Institute for Medical ResearchThe Walter and Eliza Hall Institute for Medical ResearchThe Walter and Eliza Hall Institute for Medical ResearchThe Walter and Eliza Hall Institute for Medical ResearchThe Walter and Eliza Hall Institute for Medical ResearchThe Walter and Eliza Hall Institute for Medical ResearchThe Walter and Eliza Hall Institute for Medical ResearchThe Walter and Eliza Hall Institute for Medical ResearchThe Walter and Eliza Hall Institute for Medical ResearchThe Walter and Eliza Hall Institute for Medical ResearchThe Walter and Eliza Hall Institute for Medical ResearchAmphista Therapeutics LtdChemistry School of Natural and Environmental Sciences, Bedson Building, Newcastle University Edwards WalkDivision of Biological Chemistry and Drug Discovery, School of Life Sciences, University of DundeeThe Walter and Eliza Hall Institute for Medical ResearchThe Walter and Eliza Hall Institute for Medical ResearchThe Walter and Eliza Hall Institute for Medical Researchtag-Targeted Protein Degrader (tTPD) systems are powerful tools for preclinical target validation. Here the authors extend the tTPD platform by developing NanoTACs that degrade NanoLuc tagged substrates and benchmark each tTPD system using an interchangeable tag reporter system.https://doi.org/10.1038/s41467-022-29670-1 |
| spellingShingle | Christoph Grohmann Charlene M. Magtoto Joel R. Walker Ngee Kiat Chua Anna Gabrielyan Mary Hall Simon A. Cobbold Stephen Mieruszynski Martin Brzozowski Daniel S. Simpson Hao Dong Bridget Dorizzi Annette V. Jacobsen Emma Morrish Natasha Silke James M. Murphy Joan K. Heath Andrea Testa Chiara Maniaci Alessio Ciulli Guillaume Lessene John Silke Rebecca Feltham Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms Nature Communications |
| title | Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms |
| title_full | Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms |
| title_fullStr | Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms |
| title_full_unstemmed | Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms |
| title_short | Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms |
| title_sort | development of nanoluc targeting protein degraders and a universal reporter system to benchmark tag targeted degradation platforms |
| url | https://doi.org/10.1038/s41467-022-29670-1 |
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