Leishmania major cause of a cutaneous leishmaniasis in a car driver from East Azerbaijan province
Leishmaniasis is a zoonotic disease and is known as a health problem in all of the world. The patient, a 47-year-old man car driver from the city of Bostan Abad, with a history of traveling to cutaneous leishmaniasis endemic provinces last year, was referred to the city health center with a 17 mm, n...
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| Format: | Article |
| Language: | fas |
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Tabriz University of Medical Sciences
2021-10-01
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| Series: | مجله پزشکی دانشگاه علوم پزشکی تبریز |
| Subjects: | |
| Online Access: | https://mj.tbzmed.ac.ir/PDF/mj-43-385.pdf |
| _version_ | 1828145054015815680 |
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| author | Mahdi Parsaei Shalaleh Ganji Majid Khanmohammadi |
| author_facet | Mahdi Parsaei Shalaleh Ganji Majid Khanmohammadi |
| author_sort | Mahdi Parsaei |
| collection | DOAJ |
| description | Leishmaniasis is a zoonotic disease and is known as a health problem in all of the world. The patient, a 47-year-old man car driver from the city of Bostan Abad, with a history of traveling to cutaneous leishmaniasis endemic provinces last year, was referred to the city health center with a 17 mm, no discharge and pus with a necrotic appearance skin lesion. Biochemical, hematological parameters and urine culture and analysis were normal. Three smears were prepared with sterile vaccinostyle from the margins of the wound and transferred to a specific medium in sterile conditions. After staining the prepared smears from the wound, amastigotes with a large nucleus and small kinetoplast in macrophages and active promastigotes in the liquid phase of the medium were observed. Polymerase chain reaction (PCR) was performed using ITS-1 by specific primers. In the PCR product electrophoresis was compared with the marker bands and 350 bp band weight determined. With electrophoretic patterns and comparison with positive control band, isolates parasites belonged to the Leishmania genus. In order to definitively diagnose the parasite species, a sequencing method was used. The results showed 99% homology with Leishmania major. Finally, the patient with a diagnosis of rural cutaneous leishmaniasis was induction with Glucantime drug. Diagnosis of suspected cases with cutaneous leishmaniasis is of major importance. If an infected patient is diagnosed in the early stages of the disease, the extension of the scar after recovery, as well as the possible complications of rural infection can be prevented |
| first_indexed | 2024-04-11T20:27:33Z |
| format | Article |
| id | doaj.art-cbe3ca0108cc49abb27b1bdfe1378346 |
| institution | Directory Open Access Journal |
| issn | 2783-2031 2783-204X |
| language | fas |
| last_indexed | 2024-04-11T20:27:33Z |
| publishDate | 2021-10-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | مجله پزشکی دانشگاه علوم پزشکی تبریز |
| spelling | doaj.art-cbe3ca0108cc49abb27b1bdfe13783462022-12-22T04:04:38ZfasTabriz University of Medical Sciencesمجله پزشکی دانشگاه علوم پزشکی تبریز2783-20312783-204X2021-10-0143438539310.34172/mj.2021.06527409Leishmania major cause of a cutaneous leishmaniasis in a car driver from East Azerbaijan provinceMahdi Parsaei0Shalaleh Ganji1Majid Khanmohammadi2Department of Parasitology and Mycology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IranDepartment of Pediatrics, Children’s Hospital, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Laboratory Sciences, Marand Branch, Islamic Azad University, Marand, IranLeishmaniasis is a zoonotic disease and is known as a health problem in all of the world. The patient, a 47-year-old man car driver from the city of Bostan Abad, with a history of traveling to cutaneous leishmaniasis endemic provinces last year, was referred to the city health center with a 17 mm, no discharge and pus with a necrotic appearance skin lesion. Biochemical, hematological parameters and urine culture and analysis were normal. Three smears were prepared with sterile vaccinostyle from the margins of the wound and transferred to a specific medium in sterile conditions. After staining the prepared smears from the wound, amastigotes with a large nucleus and small kinetoplast in macrophages and active promastigotes in the liquid phase of the medium were observed. Polymerase chain reaction (PCR) was performed using ITS-1 by specific primers. In the PCR product electrophoresis was compared with the marker bands and 350 bp band weight determined. With electrophoretic patterns and comparison with positive control band, isolates parasites belonged to the Leishmania genus. In order to definitively diagnose the parasite species, a sequencing method was used. The results showed 99% homology with Leishmania major. Finally, the patient with a diagnosis of rural cutaneous leishmaniasis was induction with Glucantime drug. Diagnosis of suspected cases with cutaneous leishmaniasis is of major importance. If an infected patient is diagnosed in the early stages of the disease, the extension of the scar after recovery, as well as the possible complications of rural infection can be preventedhttps://mj.tbzmed.ac.ir/PDF/mj-43-385.pdfcutaneous leishmaniasisleishmania majorwoundeast azerbaijaniran |
| spellingShingle | Mahdi Parsaei Shalaleh Ganji Majid Khanmohammadi Leishmania major cause of a cutaneous leishmaniasis in a car driver from East Azerbaijan province مجله پزشکی دانشگاه علوم پزشکی تبریز cutaneous leishmaniasis leishmania major wound east azerbaijan iran |
| title | Leishmania major cause of a cutaneous leishmaniasis in a car driver from East Azerbaijan province |
| title_full | Leishmania major cause of a cutaneous leishmaniasis in a car driver from East Azerbaijan province |
| title_fullStr | Leishmania major cause of a cutaneous leishmaniasis in a car driver from East Azerbaijan province |
| title_full_unstemmed | Leishmania major cause of a cutaneous leishmaniasis in a car driver from East Azerbaijan province |
| title_short | Leishmania major cause of a cutaneous leishmaniasis in a car driver from East Azerbaijan province |
| title_sort | leishmania major cause of a cutaneous leishmaniasis in a car driver from east azerbaijan province |
| topic | cutaneous leishmaniasis leishmania major wound east azerbaijan iran |
| url | https://mj.tbzmed.ac.ir/PDF/mj-43-385.pdf |
| work_keys_str_mv | AT mahdiparsaei leishmaniamajorcauseofacutaneousleishmaniasisinacardriverfromeastazerbaijanprovince AT shalalehganji leishmaniamajorcauseofacutaneousleishmaniasisinacardriverfromeastazerbaijanprovince AT majidkhanmohammadi leishmaniamajorcauseofacutaneousleishmaniasisinacardriverfromeastazerbaijanprovince |