Relationship between Protein Oxidation Biomarkers and Uterine Health in Dairy Cows during the Postpartum Period
High neutrophil (PMN, Polymorphonuclear neutrophil) counts in the endometrium of cows affected by endometritis, suggests the involvement of oxidative stress (OS) among the causes of impaired fertility. Protein oxidation, in particular, advanced oxidation protein products (AOPP), are OS biomarkers li...
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MDPI AG
2019-01-01
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| Series: | Antioxidants |
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| Online Access: | http://www.mdpi.com/2076-3921/8/1/21 |
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| author | Gianfranco Gabai Elisabetta De Luca Giovanni Miotto Gianni Zin Annalisa Stefani Laura Da Dalt Antonio Barberio Pietro Celi |
| author_facet | Gianfranco Gabai Elisabetta De Luca Giovanni Miotto Gianni Zin Annalisa Stefani Laura Da Dalt Antonio Barberio Pietro Celi |
| author_sort | Gianfranco Gabai |
| collection | DOAJ |
| description | High neutrophil (PMN, Polymorphonuclear neutrophil) counts in the endometrium of cows affected by endometritis, suggests the involvement of oxidative stress (OS) among the causes of impaired fertility. Protein oxidation, in particular, advanced oxidation protein products (AOPP), are OS biomarkers linked to PMN activity. To test this hypothesis, the relationship between protein oxidation and uterus health was studied in thirty-eight dairy cows during the puerperium. The animals were found to be cycling, without any signs of disease and pharmacological treatments. PMN count was performed either through a cytobrush or a uterine horn lavage (UHL). Cows were classified into four groups, based on the uterine ultrasonographic characteristics and the PMN percentage in the uterine horns with a higher percentage of high neutrophil horn (HNH). They were classified as: Healthy (H); Subclinical Endometritis (SCE); Grade 1 Endometritis (EM1); and Grade 2 Endometritis (EM2). AOPP and carbonyls were measured in plasma and UHL. UHL samples underwent Western blot analysis to visualize the carbonyl and dityrosine formation. Plasma AOPP were higher (p < 0.05) in EM2. AOPP and carbonyl group concentrations were higher in the HNH samples (p < 0.05). Protein concentration in the UHL was higher in the EM2 (p < 0.05). Carbonyl and dityrosine formation was more intense in EM1 and EM2. Protein oxidation observed in the EM2 suggests the presence of an inflammatory status in the uterus which, if not adequately hindered, could result in low fertility. |
| first_indexed | 2024-03-12T06:01:06Z |
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| id | doaj.art-cbe720e5b6ea4f3494cc3a5bac516196 |
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| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T06:01:06Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj.art-cbe720e5b6ea4f3494cc3a5bac5161962023-09-03T04:07:53ZengMDPI AGAntioxidants2076-39212019-01-01812110.3390/antiox8010021antiox8010021Relationship between Protein Oxidation Biomarkers and Uterine Health in Dairy Cows during the Postpartum PeriodGianfranco Gabai0Elisabetta De Luca1Giovanni Miotto2Gianni Zin3Annalisa Stefani4Laura Da Dalt5Antonio Barberio6Pietro Celi7Department of Comparative Biomedicine and Food Science, University of Padova, 35020 Legnaro (PD), ItalyDepartment of Comparative Biomedicine and Food Science, University of Padova, 35020 Legnaro (PD), ItalyDepartment of Molecular Medicine, University of Padova, 35121 Padova, ItalyVia Argine Sinistro 23 Creola di Saccolongo 35030 (PD), ItalyIstituto Zooprofilattico Sperimentale delle Venezie—Italian Health Authority and Research Organization for Animal Health and Food Safety, 35020Legnaro (PD), ItalyDepartment of Comparative Biomedicine and Food Science, University of Padova, 35020 Legnaro (PD), ItalyIstituto Zooprofilattico Sperimentale delle Venezie—Italian Health Authority and Research Organization for Animal Health and Food Safety, 35020Legnaro (PD), ItalyDSM Nutritional Products, 4303 Kaiseraugst, SwitzerlandHigh neutrophil (PMN, Polymorphonuclear neutrophil) counts in the endometrium of cows affected by endometritis, suggests the involvement of oxidative stress (OS) among the causes of impaired fertility. Protein oxidation, in particular, advanced oxidation protein products (AOPP), are OS biomarkers linked to PMN activity. To test this hypothesis, the relationship between protein oxidation and uterus health was studied in thirty-eight dairy cows during the puerperium. The animals were found to be cycling, without any signs of disease and pharmacological treatments. PMN count was performed either through a cytobrush or a uterine horn lavage (UHL). Cows were classified into four groups, based on the uterine ultrasonographic characteristics and the PMN percentage in the uterine horns with a higher percentage of high neutrophil horn (HNH). They were classified as: Healthy (H); Subclinical Endometritis (SCE); Grade 1 Endometritis (EM1); and Grade 2 Endometritis (EM2). AOPP and carbonyls were measured in plasma and UHL. UHL samples underwent Western blot analysis to visualize the carbonyl and dityrosine formation. Plasma AOPP were higher (p < 0.05) in EM2. AOPP and carbonyl group concentrations were higher in the HNH samples (p < 0.05). Protein concentration in the UHL was higher in the EM2 (p < 0.05). Carbonyl and dityrosine formation was more intense in EM1 and EM2. Protein oxidation observed in the EM2 suggests the presence of an inflammatory status in the uterus which, if not adequately hindered, could result in low fertility.http://www.mdpi.com/2076-3921/8/1/21dairy cowpuerperiumuterusclinical outcomeoxidized proteins |
| spellingShingle | Gianfranco Gabai Elisabetta De Luca Giovanni Miotto Gianni Zin Annalisa Stefani Laura Da Dalt Antonio Barberio Pietro Celi Relationship between Protein Oxidation Biomarkers and Uterine Health in Dairy Cows during the Postpartum Period Antioxidants dairy cow puerperium uterus clinical outcome oxidized proteins |
| title | Relationship between Protein Oxidation Biomarkers and Uterine Health in Dairy Cows during the Postpartum Period |
| title_full | Relationship between Protein Oxidation Biomarkers and Uterine Health in Dairy Cows during the Postpartum Period |
| title_fullStr | Relationship between Protein Oxidation Biomarkers and Uterine Health in Dairy Cows during the Postpartum Period |
| title_full_unstemmed | Relationship between Protein Oxidation Biomarkers and Uterine Health in Dairy Cows during the Postpartum Period |
| title_short | Relationship between Protein Oxidation Biomarkers and Uterine Health in Dairy Cows during the Postpartum Period |
| title_sort | relationship between protein oxidation biomarkers and uterine health in dairy cows during the postpartum period |
| topic | dairy cow puerperium uterus clinical outcome oxidized proteins |
| url | http://www.mdpi.com/2076-3921/8/1/21 |
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