Macroautophagy abnormality in essential tremor.
Macroautophagy is a cellular mechanism for the clearance of protein aggregates and damaged organelles. Impaired macroautophagy has been observed in neurodegenerative disorders. We investigated the macroautophagy pathway in essential tremor (ET) cases compared to age-matched controls. We analyzed mic...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3531444?pdf=render |
| _version_ | 1828482604665405440 |
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| author | Sheng-Han Kuo Guomei Tang Karen Ma Rachel Babij Etty Cortes Jean-Paul G Vonsattel Phyllis L Faust David Sulzer Elan D Louis |
| author_facet | Sheng-Han Kuo Guomei Tang Karen Ma Rachel Babij Etty Cortes Jean-Paul G Vonsattel Phyllis L Faust David Sulzer Elan D Louis |
| author_sort | Sheng-Han Kuo |
| collection | DOAJ |
| description | Macroautophagy is a cellular mechanism for the clearance of protein aggregates and damaged organelles. Impaired macroautophagy has been observed in neurodegenerative disorders. We investigated the macroautophagy pathway in essential tremor (ET) cases compared to age-matched controls. We analyzed microtubule-associated protein light chain 3-II (LC3-II), S6K, phosphorylated S6K, beclin-1, and mitochondrial membrane proteins levels by Western blot in the post-mortem cerebellum of 10 ET cases and 11 controls. We also performed immunohistochemistry in 12 ET cases and 13 controls to quantify LC3 clustering in Purkinje cells (PCs). LC3-II protein levels were significantly lower in ET cases vs. controls on Western blot (0.84 ± 0.14 vs. 1.00 ± 0.14, p = 0.02), and LC3-II clustering in PCs by immunohistochemistry was significantly lower in ET cases vs. controls (2.03 ± 3.45 vs. 8.80 ± 9.81, p = 0.03). In ET cases, disease duration was inversely correlated with LC3-II protein level (r = -0.64, p = 0.046). We found that mitochondrial membrane proteins were accumulated in ET (TIM23: 1.36 ± 0.11 in ET cases vs. 1.00 ± 0.08 in controls, p = 0.02; TOMM20: 1.63 ± 0.87 in ET cases vs. 1.00 ± 0.14 in controls, p = 0.03). Beclin-1, which is involved in macroautophagy, was strikingly deficient in ET (0.42 ± 0.13 vs. 1.00 ± 0.35, p<0.001). Decreased macroautophagy was observed in the ET cerebellum, and this could be due to a decrease in beclin-1 levels, which subsequently lead to mitochondrial accumulation as a result of autophagic failure. This provides a possible means by which perturbed macroautophagy could contribute to PC pathology in ET. |
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| id | doaj.art-cbe8c4cf650b41f5a3958693d76f994d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T08:17:54Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-cbe8c4cf650b41f5a3958693d76f994d2022-12-22T01:14:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5304010.1371/journal.pone.0053040Macroautophagy abnormality in essential tremor.Sheng-Han KuoGuomei TangKaren MaRachel BabijEtty CortesJean-Paul G VonsattelPhyllis L FaustDavid SulzerElan D LouisMacroautophagy is a cellular mechanism for the clearance of protein aggregates and damaged organelles. Impaired macroautophagy has been observed in neurodegenerative disorders. We investigated the macroautophagy pathway in essential tremor (ET) cases compared to age-matched controls. We analyzed microtubule-associated protein light chain 3-II (LC3-II), S6K, phosphorylated S6K, beclin-1, and mitochondrial membrane proteins levels by Western blot in the post-mortem cerebellum of 10 ET cases and 11 controls. We also performed immunohistochemistry in 12 ET cases and 13 controls to quantify LC3 clustering in Purkinje cells (PCs). LC3-II protein levels were significantly lower in ET cases vs. controls on Western blot (0.84 ± 0.14 vs. 1.00 ± 0.14, p = 0.02), and LC3-II clustering in PCs by immunohistochemistry was significantly lower in ET cases vs. controls (2.03 ± 3.45 vs. 8.80 ± 9.81, p = 0.03). In ET cases, disease duration was inversely correlated with LC3-II protein level (r = -0.64, p = 0.046). We found that mitochondrial membrane proteins were accumulated in ET (TIM23: 1.36 ± 0.11 in ET cases vs. 1.00 ± 0.08 in controls, p = 0.02; TOMM20: 1.63 ± 0.87 in ET cases vs. 1.00 ± 0.14 in controls, p = 0.03). Beclin-1, which is involved in macroautophagy, was strikingly deficient in ET (0.42 ± 0.13 vs. 1.00 ± 0.35, p<0.001). Decreased macroautophagy was observed in the ET cerebellum, and this could be due to a decrease in beclin-1 levels, which subsequently lead to mitochondrial accumulation as a result of autophagic failure. This provides a possible means by which perturbed macroautophagy could contribute to PC pathology in ET.http://europepmc.org/articles/PMC3531444?pdf=render |
| spellingShingle | Sheng-Han Kuo Guomei Tang Karen Ma Rachel Babij Etty Cortes Jean-Paul G Vonsattel Phyllis L Faust David Sulzer Elan D Louis Macroautophagy abnormality in essential tremor. PLoS ONE |
| title | Macroautophagy abnormality in essential tremor. |
| title_full | Macroautophagy abnormality in essential tremor. |
| title_fullStr | Macroautophagy abnormality in essential tremor. |
| title_full_unstemmed | Macroautophagy abnormality in essential tremor. |
| title_short | Macroautophagy abnormality in essential tremor. |
| title_sort | macroautophagy abnormality in essential tremor |
| url | http://europepmc.org/articles/PMC3531444?pdf=render |
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