Circ_0006174 Accelerates Colorectal Cancer Progression Through Regulating miR-138-5p/MACC1 Axis
Jianjun Wei,* Yuzhe Lin,* Zhiqiang Wang, Yeguang Liu, Wei Guo Minimally Invasive Surgery, Linhe People’s Hospital of Bayannur City, Bayannur, Inner Mongolia, 015000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhiqiang Wang Tel +86-047...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-02-01
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| Series: | Cancer Management and Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/circ0006174-accelerates-colorectal-cancer-progression-through-regulati-peer-reviewed-article-CMAR |
| _version_ | 1818672982093987840 |
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| author | Wei J Lin Y Wang Z Liu Y Guo W |
| author_facet | Wei J Lin Y Wang Z Liu Y Guo W |
| author_sort | Wei J |
| collection | DOAJ |
| description | Jianjun Wei,* Yuzhe Lin,* Zhiqiang Wang, Yeguang Liu, Wei Guo Minimally Invasive Surgery, Linhe People’s Hospital of Bayannur City, Bayannur, Inner Mongolia, 015000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhiqiang Wang Tel +86-04788253451Email zqwang470018979@163.comBackground: Circular RNAs (circRNAs) were reported to be involved in the progression of a variety of cancers, including colorectal cancer (CRC). However, the precise functions and mechanism of circRNAs in CRC have not been elucidated. This study aimed to investigate the effect and mechanism underlying circ_0006174 in CRC.Methods: The expression of circ_0006174, microRNA (miR-138-5p) and metastasis associated in colon cancer 1 (MACC1) mRNA was detected by quantitative real-time polymerase chain reaction (RT-qPCR) assay. Western blot was employed to measure MACC1 protein expression. The effects of circ_0006174 knockdown, MACC1 overexpression or miR-138-5p inhibition on cell proliferation, migration, invasion, and apoptosis were assessed by cell counting kit 8 (CCK-8) assay, clone formation assay, transwell assay and flow cytometry assay, respectively. The interaction between miR-138-5p and circ_0006174 or MACC1 was confirmed by RNA pull down assay or dual-luciferase reporter assay. Xenograft tumor model in nude mice was used to verify the function of circ_0006174 in vivo.Results: Circ_0006174 and MACC1 expression was highly expressed, while miR-138-5p expression was downregulated in CRC cells and tissues. Meanwhile, circ_0006174 functioned as a sponge of miR-138-5p to upregulate MACC1 expression. Furthermore, circ_0006174 knock down-mediated suppression on cell proliferation, migration and invasion, and promotion on cell apoptosis could be alleviated by MACC1 overexpression or miR-138-5p inhibition in CRC cells. Besides, circ_0006174 knockdown also inhibited CRC procession in vivo.Conclusion: Circ_0006174 advanced CRC progression via sponging miR-138-5p to upregulate MACC1 expression, which may provide a promising molecular target for CRC treatment.Keywords: colorectal cancer, circ_0006174, miR-138-5p, MACC1 |
| first_indexed | 2024-12-17T07:48:32Z |
| format | Article |
| id | doaj.art-cbeb7f667b5a42a3ae65e961d4a8905d |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-17T07:48:32Z |
| publishDate | 2021-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-cbeb7f667b5a42a3ae65e961d4a8905d2022-12-21T21:57:55ZengDove Medical PressCancer Management and Research1179-13222021-02-01Volume 131673168662292Circ_0006174 Accelerates Colorectal Cancer Progression Through Regulating miR-138-5p/MACC1 AxisWei JLin YWang ZLiu YGuo WJianjun Wei,* Yuzhe Lin,* Zhiqiang Wang, Yeguang Liu, Wei Guo Minimally Invasive Surgery, Linhe People’s Hospital of Bayannur City, Bayannur, Inner Mongolia, 015000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhiqiang Wang Tel +86-04788253451Email zqwang470018979@163.comBackground: Circular RNAs (circRNAs) were reported to be involved in the progression of a variety of cancers, including colorectal cancer (CRC). However, the precise functions and mechanism of circRNAs in CRC have not been elucidated. This study aimed to investigate the effect and mechanism underlying circ_0006174 in CRC.Methods: The expression of circ_0006174, microRNA (miR-138-5p) and metastasis associated in colon cancer 1 (MACC1) mRNA was detected by quantitative real-time polymerase chain reaction (RT-qPCR) assay. Western blot was employed to measure MACC1 protein expression. The effects of circ_0006174 knockdown, MACC1 overexpression or miR-138-5p inhibition on cell proliferation, migration, invasion, and apoptosis were assessed by cell counting kit 8 (CCK-8) assay, clone formation assay, transwell assay and flow cytometry assay, respectively. The interaction between miR-138-5p and circ_0006174 or MACC1 was confirmed by RNA pull down assay or dual-luciferase reporter assay. Xenograft tumor model in nude mice was used to verify the function of circ_0006174 in vivo.Results: Circ_0006174 and MACC1 expression was highly expressed, while miR-138-5p expression was downregulated in CRC cells and tissues. Meanwhile, circ_0006174 functioned as a sponge of miR-138-5p to upregulate MACC1 expression. Furthermore, circ_0006174 knock down-mediated suppression on cell proliferation, migration and invasion, and promotion on cell apoptosis could be alleviated by MACC1 overexpression or miR-138-5p inhibition in CRC cells. Besides, circ_0006174 knockdown also inhibited CRC procession in vivo.Conclusion: Circ_0006174 advanced CRC progression via sponging miR-138-5p to upregulate MACC1 expression, which may provide a promising molecular target for CRC treatment.Keywords: colorectal cancer, circ_0006174, miR-138-5p, MACC1https://www.dovepress.com/circ0006174-accelerates-colorectal-cancer-progression-through-regulati-peer-reviewed-article-CMARcolorectal cancercirc_0006174mir-138-5pmacc1 |
| spellingShingle | Wei J Lin Y Wang Z Liu Y Guo W Circ_0006174 Accelerates Colorectal Cancer Progression Through Regulating miR-138-5p/MACC1 Axis Cancer Management and Research colorectal cancer circ_0006174 mir-138-5p macc1 |
| title | Circ_0006174 Accelerates Colorectal Cancer Progression Through Regulating miR-138-5p/MACC1 Axis |
| title_full | Circ_0006174 Accelerates Colorectal Cancer Progression Through Regulating miR-138-5p/MACC1 Axis |
| title_fullStr | Circ_0006174 Accelerates Colorectal Cancer Progression Through Regulating miR-138-5p/MACC1 Axis |
| title_full_unstemmed | Circ_0006174 Accelerates Colorectal Cancer Progression Through Regulating miR-138-5p/MACC1 Axis |
| title_short | Circ_0006174 Accelerates Colorectal Cancer Progression Through Regulating miR-138-5p/MACC1 Axis |
| title_sort | circ 0006174 accelerates colorectal cancer progression through regulating mir 138 5p macc1 axis |
| topic | colorectal cancer circ_0006174 mir-138-5p macc1 |
| url | https://www.dovepress.com/circ0006174-accelerates-colorectal-cancer-progression-through-regulati-peer-reviewed-article-CMAR |
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