Synthetic Biology Tools for Engineering Microbial Cells to Fight Superbugs
With the increase in clinical cases of bacterial infections with multiple antibiotic resistance, the world has entered a health crisis. Overuse, inappropriate prescribing, and lack of innovation of antibiotics have contributed to the surge of microorganisms that can overcome traditional antimicrobia...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.869206/full |
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| author | Angel León-Buitimea Angel León-Buitimea Francisco de Jesús Balderas-Cisneros Francisco de Jesús Balderas-Cisneros César Rodolfo Garza-Cárdenas César Rodolfo Garza-Cárdenas Javier Alberto Garza-Cervantes Javier Alberto Garza-Cervantes José Rubén Morones-Ramírez José Rubén Morones-Ramírez |
| author_facet | Angel León-Buitimea Angel León-Buitimea Francisco de Jesús Balderas-Cisneros Francisco de Jesús Balderas-Cisneros César Rodolfo Garza-Cárdenas César Rodolfo Garza-Cárdenas Javier Alberto Garza-Cervantes Javier Alberto Garza-Cervantes José Rubén Morones-Ramírez José Rubén Morones-Ramírez |
| author_sort | Angel León-Buitimea |
| collection | DOAJ |
| description | With the increase in clinical cases of bacterial infections with multiple antibiotic resistance, the world has entered a health crisis. Overuse, inappropriate prescribing, and lack of innovation of antibiotics have contributed to the surge of microorganisms that can overcome traditional antimicrobial treatments. In 2017, the World Health Organization published a list of pathogenic bacteria, including Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Escherichia coli (ESKAPE). These bacteria can adapt to multiple antibiotics and transfer their resistance to other organisms; therefore, studies to find new therapeutic strategies are needed. One of these strategies is synthetic biology geared toward developing new antimicrobial therapies. Synthetic biology is founded on a solid and well-established theoretical framework that provides tools for conceptualizing, designing, and constructing synthetic biological systems. Recent developments in synthetic biology provide tools for engineering synthetic control systems in microbial cells. Applying protein engineering, DNA synthesis, and in silico design allows building metabolic pathways and biological circuits to control cellular behavior. Thus, synthetic biology advances have permitted the construction of communication systems between microorganisms where exogenous molecules can control specific population behaviors, induce intracellular signaling, and establish co-dependent networks of microorganisms. |
| first_indexed | 2024-12-12T04:05:14Z |
| format | Article |
| id | doaj.art-cbec9ba0fb5241ba8590fd851c24855b |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-12T04:05:14Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-cbec9ba0fb5241ba8590fd851c24855b2022-12-22T00:38:48ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-05-011010.3389/fbioe.2022.869206869206Synthetic Biology Tools for Engineering Microbial Cells to Fight SuperbugsAngel León-Buitimea0Angel León-Buitimea1Francisco de Jesús Balderas-Cisneros2Francisco de Jesús Balderas-Cisneros3César Rodolfo Garza-Cárdenas4César Rodolfo Garza-Cárdenas5Javier Alberto Garza-Cervantes6Javier Alberto Garza-Cervantes7José Rubén Morones-Ramírez8José Rubén Morones-Ramírez9Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León (UANL), San Nicolás de los Garza, MexicoCentro de Investigación en Biotecnología y Nanotecnología, Facultad de Ciencias Químicas, Parque de Investigación e Innovación Tecnológica, Universidad Autónoma de Nuevo León, Apodaca, MexicoFacultad de Ciencias Químicas, Universidad Autónoma de Nuevo León (UANL), San Nicolás de los Garza, MexicoCentro de Investigación en Biotecnología y Nanotecnología, Facultad de Ciencias Químicas, Parque de Investigación e Innovación Tecnológica, Universidad Autónoma de Nuevo León, Apodaca, MexicoFacultad de Ciencias Químicas, Universidad Autónoma de Nuevo León (UANL), San Nicolás de los Garza, MexicoCentro de Investigación en Biotecnología y Nanotecnología, Facultad de Ciencias Químicas, Parque de Investigación e Innovación Tecnológica, Universidad Autónoma de Nuevo León, Apodaca, MexicoFacultad de Ciencias Químicas, Universidad Autónoma de Nuevo León (UANL), San Nicolás de los Garza, MexicoCentro de Investigación en Biotecnología y Nanotecnología, Facultad de Ciencias Químicas, Parque de Investigación e Innovación Tecnológica, Universidad Autónoma de Nuevo León, Apodaca, MexicoFacultad de Ciencias Químicas, Universidad Autónoma de Nuevo León (UANL), San Nicolás de los Garza, MexicoCentro de Investigación en Biotecnología y Nanotecnología, Facultad de Ciencias Químicas, Parque de Investigación e Innovación Tecnológica, Universidad Autónoma de Nuevo León, Apodaca, MexicoWith the increase in clinical cases of bacterial infections with multiple antibiotic resistance, the world has entered a health crisis. Overuse, inappropriate prescribing, and lack of innovation of antibiotics have contributed to the surge of microorganisms that can overcome traditional antimicrobial treatments. In 2017, the World Health Organization published a list of pathogenic bacteria, including Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Escherichia coli (ESKAPE). These bacteria can adapt to multiple antibiotics and transfer their resistance to other organisms; therefore, studies to find new therapeutic strategies are needed. One of these strategies is synthetic biology geared toward developing new antimicrobial therapies. Synthetic biology is founded on a solid and well-established theoretical framework that provides tools for conceptualizing, designing, and constructing synthetic biological systems. Recent developments in synthetic biology provide tools for engineering synthetic control systems in microbial cells. Applying protein engineering, DNA synthesis, and in silico design allows building metabolic pathways and biological circuits to control cellular behavior. Thus, synthetic biology advances have permitted the construction of communication systems between microorganisms where exogenous molecules can control specific population behaviors, induce intracellular signaling, and establish co-dependent networks of microorganisms.https://www.frontiersin.org/articles/10.3389/fbioe.2022.869206/fullsynthetic biologyantimicrobial resistancegenetic circuitsantibioticsphageswhole-cell engineering |
| spellingShingle | Angel León-Buitimea Angel León-Buitimea Francisco de Jesús Balderas-Cisneros Francisco de Jesús Balderas-Cisneros César Rodolfo Garza-Cárdenas César Rodolfo Garza-Cárdenas Javier Alberto Garza-Cervantes Javier Alberto Garza-Cervantes José Rubén Morones-Ramírez José Rubén Morones-Ramírez Synthetic Biology Tools for Engineering Microbial Cells to Fight Superbugs Frontiers in Bioengineering and Biotechnology synthetic biology antimicrobial resistance genetic circuits antibiotics phages whole-cell engineering |
| title | Synthetic Biology Tools for Engineering Microbial Cells to Fight Superbugs |
| title_full | Synthetic Biology Tools for Engineering Microbial Cells to Fight Superbugs |
| title_fullStr | Synthetic Biology Tools for Engineering Microbial Cells to Fight Superbugs |
| title_full_unstemmed | Synthetic Biology Tools for Engineering Microbial Cells to Fight Superbugs |
| title_short | Synthetic Biology Tools for Engineering Microbial Cells to Fight Superbugs |
| title_sort | synthetic biology tools for engineering microbial cells to fight superbugs |
| topic | synthetic biology antimicrobial resistance genetic circuits antibiotics phages whole-cell engineering |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.869206/full |
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