In silico Approach in the Prediction and Analysis of the Three-dimensional Structure of Maleylacetate reductase: A Biodegrading Protein
With the advent of biological research in the field of environmental science, several microbes were found to act as the most important biodegradable molecules. Maleylacetate reductase being a member of oxidoreductase is mostly found in Pseudomonas species. This enzyme participates in three metabolic...
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| Format: | Article |
| Language: | English |
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Bulgarian Academy of Sciences
2013-12-01
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| Series: | International Journal Bioautomation |
| Subjects: | |
| Online Access: | http://www.biomed.bas.bg/bioautomation/2013/vol_17.4/files/17.4_02.pdf |
| _version_ | 1811337412273504256 |
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| author | Shasank Sekhar Swain |
| author_facet | Shasank Sekhar Swain |
| author_sort | Shasank Sekhar Swain |
| collection | DOAJ |
| description | With the advent of biological research in the field of environmental science, several microbes were found to act as the most important biodegradable molecules. Maleylacetate reductase being a member of oxidoreductase is mostly found in Pseudomonas species. This enzyme participates in three metabolic pathways: gamma-hexachlorocyclohexane degradation, benzoate degradation which are higher alkyl compounds involved in chemical pollutions in metropolitan cities. Determining its three-dimensional structure will lead to the structure function analysis and also might be helpful for designing receptors for the degradation of new chemical compounds. For this purpose, the amino acid sequence of the protein had been imported from UniProtKB database and the template was searched in BLASTp2.2.27. This template was then used for the prediction of the three dimensional structure of the protein by using SWISS-MODEL Workspace. The predicted model was then validated using SAVES server which gave an almost result for better prediction. |
| first_indexed | 2024-04-13T17:54:31Z |
| format | Article |
| id | doaj.art-cbefabe6ebfe4497afec2ff7b725f563 |
| institution | Directory Open Access Journal |
| issn | 1314-1902 1314-2321 |
| language | English |
| last_indexed | 2024-04-13T17:54:31Z |
| publishDate | 2013-12-01 |
| publisher | Bulgarian Academy of Sciences |
| record_format | Article |
| series | International Journal Bioautomation |
| spelling | doaj.art-cbefabe6ebfe4497afec2ff7b725f5632022-12-22T02:36:33ZengBulgarian Academy of SciencesInternational Journal Bioautomation1314-19021314-23212013-12-01174217226In silico Approach in the Prediction and Analysis of the Three-dimensional Structure of Maleylacetate reductase: A Biodegrading ProteinShasank Sekhar Swain0Research and Development Center, Hi-tech Medical College and Hospital, Bhubaneswar, Odisha, India-751010With the advent of biological research in the field of environmental science, several microbes were found to act as the most important biodegradable molecules. Maleylacetate reductase being a member of oxidoreductase is mostly found in Pseudomonas species. This enzyme participates in three metabolic pathways: gamma-hexachlorocyclohexane degradation, benzoate degradation which are higher alkyl compounds involved in chemical pollutions in metropolitan cities. Determining its three-dimensional structure will lead to the structure function analysis and also might be helpful for designing receptors for the degradation of new chemical compounds. For this purpose, the amino acid sequence of the protein had been imported from UniProtKB database and the template was searched in BLASTp2.2.27. This template was then used for the prediction of the three dimensional structure of the protein by using SWISS-MODEL Workspace. The predicted model was then validated using SAVES server which gave an almost result for better prediction.http://www.biomed.bas.bg/bioautomation/2013/vol_17.4/files/17.4_02.pdfMaleylacetate reductaseBLASTpSWISS-MODEL WorkspaceUniProtKBSAVES server |
| spellingShingle | Shasank Sekhar Swain In silico Approach in the Prediction and Analysis of the Three-dimensional Structure of Maleylacetate reductase: A Biodegrading Protein International Journal Bioautomation Maleylacetate reductase BLASTp SWISS-MODEL Workspace UniProtKB SAVES server |
| title | In silico Approach in the Prediction and Analysis of the Three-dimensional Structure of Maleylacetate reductase: A Biodegrading Protein |
| title_full | In silico Approach in the Prediction and Analysis of the Three-dimensional Structure of Maleylacetate reductase: A Biodegrading Protein |
| title_fullStr | In silico Approach in the Prediction and Analysis of the Three-dimensional Structure of Maleylacetate reductase: A Biodegrading Protein |
| title_full_unstemmed | In silico Approach in the Prediction and Analysis of the Three-dimensional Structure of Maleylacetate reductase: A Biodegrading Protein |
| title_short | In silico Approach in the Prediction and Analysis of the Three-dimensional Structure of Maleylacetate reductase: A Biodegrading Protein |
| title_sort | in silico approach in the prediction and analysis of the three dimensional structure of maleylacetate reductase a biodegrading protein |
| topic | Maleylacetate reductase BLASTp SWISS-MODEL Workspace UniProtKB SAVES server |
| url | http://www.biomed.bas.bg/bioautomation/2013/vol_17.4/files/17.4_02.pdf |
| work_keys_str_mv | AT shasanksekharswain insilicoapproachinthepredictionandanalysisofthethreedimensionalstructureofmaleylacetatereductaseabiodegradingprotein |