Genetic polymorphism characteristics of Brucella canis isolated in China.
In China, brucellosis is an endemic disease typically caused by Brucella melitensis infection (biovars 1 and 3). Brucella canis infection in dogs has not traditionally recognized as a major problem. In recent years however, brucellosis resulting from Brucella canis infection has also been reported,...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3900416?pdf=render |
| _version_ | 1818250164862713856 |
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| author | Dongdong Di Buyun Cui Heng Wang Hongyan Zhao Dongri Piao Lili Tian Guozhong Tian Jingli Kang Xiang Mao Xiaojun Zhang Pengfei Du Lin Zhu Zhuo Zhao Lingling Mao Wenqing Yao Pingyuan Guan Weixing Fan Hai Jiang |
| author_facet | Dongdong Di Buyun Cui Heng Wang Hongyan Zhao Dongri Piao Lili Tian Guozhong Tian Jingli Kang Xiang Mao Xiaojun Zhang Pengfei Du Lin Zhu Zhuo Zhao Lingling Mao Wenqing Yao Pingyuan Guan Weixing Fan Hai Jiang |
| author_sort | Dongdong Di |
| collection | DOAJ |
| description | In China, brucellosis is an endemic disease typically caused by Brucella melitensis infection (biovars 1 and 3). Brucella canis infection in dogs has not traditionally recognized as a major problem. In recent years however, brucellosis resulting from Brucella canis infection has also been reported, suggesting that infections from this species may be increasing. Data concerning the epidemiology of brucellosis resulting from Brucella canis infection is limited. Therefore, the purpose of this study was to assess the diversity among Chinese Brucella canis strains for epidemiological purposes. First, we employed a 16-marker VNTR assay (Brucella MLVA-16) to assess the diversity and epidemiological relationship of 29 Brucella canis isolates from diverse locations throughout China with 38 isolates from other countries. MLVA-16 analysis separated the 67 Brucella canis isolates into 57 genotypes that grouped into five clusters with genetic similarity coefficients ranging from 67.73 to 100%. Moreover, this analysis revealed a new genotype (2-3-9-11-3-1-5-1:118), which was present in two isolates recovered from Guangxi in 1986 and 1987. Second, multiplex PCR and sequencing analysis were used to determine whether the 29 Chinese Brucella canis isolates had the characteristic BMEI1435 gene deletion. Only two isolates had this deletion. Third, amplification of the omp25 gene revealed that 26 isolates from China had a T545C mutation. Collectively, this study reveals that considerable diversity exists among Brucella canis isolates in China and provides resources for studying the genetic variation and microevolution of Brucella. |
| first_indexed | 2024-12-12T15:48:03Z |
| format | Article |
| id | doaj.art-cbf17daba54f4f7f92d685a74e26dcf5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T15:48:03Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cbf17daba54f4f7f92d685a74e26dcf52022-12-22T00:19:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8486210.1371/journal.pone.0084862Genetic polymorphism characteristics of Brucella canis isolated in China.Dongdong DiBuyun CuiHeng WangHongyan ZhaoDongri PiaoLili TianGuozhong TianJingli KangXiang MaoXiaojun ZhangPengfei DuLin ZhuZhuo ZhaoLingling MaoWenqing YaoPingyuan GuanWeixing FanHai JiangIn China, brucellosis is an endemic disease typically caused by Brucella melitensis infection (biovars 1 and 3). Brucella canis infection in dogs has not traditionally recognized as a major problem. In recent years however, brucellosis resulting from Brucella canis infection has also been reported, suggesting that infections from this species may be increasing. Data concerning the epidemiology of brucellosis resulting from Brucella canis infection is limited. Therefore, the purpose of this study was to assess the diversity among Chinese Brucella canis strains for epidemiological purposes. First, we employed a 16-marker VNTR assay (Brucella MLVA-16) to assess the diversity and epidemiological relationship of 29 Brucella canis isolates from diverse locations throughout China with 38 isolates from other countries. MLVA-16 analysis separated the 67 Brucella canis isolates into 57 genotypes that grouped into five clusters with genetic similarity coefficients ranging from 67.73 to 100%. Moreover, this analysis revealed a new genotype (2-3-9-11-3-1-5-1:118), which was present in two isolates recovered from Guangxi in 1986 and 1987. Second, multiplex PCR and sequencing analysis were used to determine whether the 29 Chinese Brucella canis isolates had the characteristic BMEI1435 gene deletion. Only two isolates had this deletion. Third, amplification of the omp25 gene revealed that 26 isolates from China had a T545C mutation. Collectively, this study reveals that considerable diversity exists among Brucella canis isolates in China and provides resources for studying the genetic variation and microevolution of Brucella.http://europepmc.org/articles/PMC3900416?pdf=render |
| spellingShingle | Dongdong Di Buyun Cui Heng Wang Hongyan Zhao Dongri Piao Lili Tian Guozhong Tian Jingli Kang Xiang Mao Xiaojun Zhang Pengfei Du Lin Zhu Zhuo Zhao Lingling Mao Wenqing Yao Pingyuan Guan Weixing Fan Hai Jiang Genetic polymorphism characteristics of Brucella canis isolated in China. PLoS ONE |
| title | Genetic polymorphism characteristics of Brucella canis isolated in China. |
| title_full | Genetic polymorphism characteristics of Brucella canis isolated in China. |
| title_fullStr | Genetic polymorphism characteristics of Brucella canis isolated in China. |
| title_full_unstemmed | Genetic polymorphism characteristics of Brucella canis isolated in China. |
| title_short | Genetic polymorphism characteristics of Brucella canis isolated in China. |
| title_sort | genetic polymorphism characteristics of brucella canis isolated in china |
| url | http://europepmc.org/articles/PMC3900416?pdf=render |
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