Evaluation of an automated light transmission aggregometry
Light transmission aggregometry (LTA) is the “gold standard” for platelet function assessment, but it is time-consuming and labor intensive. Recently, an automated platelet aggregation method has been developed on a routine coagulation analyzer (Sysmex CS-2100i). In this study, the performances of C...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-10-01
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| Series: | Platelets |
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| Online Access: | http://dx.doi.org/10.1080/09537104.2016.1265923 |
| _version_ | 1797684296203370496 |
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| author | Li-qin Ling Juan Liao Qian Niu Xia Wang Jin Jia Cheng-hua Zuo Hong Jiang Jing Zhou |
| author_facet | Li-qin Ling Juan Liao Qian Niu Xia Wang Jin Jia Cheng-hua Zuo Hong Jiang Jing Zhou |
| author_sort | Li-qin Ling |
| collection | DOAJ |
| description | Light transmission aggregometry (LTA) is the “gold standard” for platelet function assessment, but it is time-consuming and labor intensive. Recently, an automated platelet aggregation method has been developed on a routine coagulation analyzer (Sysmex CS-2100i). In this study, the performances of CS-2100i including repeatability, correlation with a reference aggregometer (Chrono-log Model 700), and the threshold limitation of platelet counts in platelet-rich plasma (PRP) were evaluated for clinical use. The agonists were adenosine diphosphate (ADP), arachidonic acid, collagen, epinephrine, and ristocetin. The platelet concentration of PRP was adjusted with platelet-poor plasma (PPP) and physiological saline (PS). The CS-2100i showed an excellent repeatability and a strong correlation with the Chrono-log 700 in performing platelet aggregation, and its threshold limitation of platelet counts in PRP was 80 × 109/L. PPP had an inhibitory impact on platelet aggregation induced by ADP, arachidonic acid, collagen or epinephrine; while PS had an inhibitory impact on ristocetin-induced aggregation. PS should be used to adjust PRP for ADP-, arachidonic acid-, collagen-, or epinephrine-induced aggregation; while PPP was recommended for ristocetin-induced aggregation. The CS-2100i showed an excellent repeatability, a strong correlation with Chrono-log 700, a lower platelet count requirement, a shorter turnaround time for samples, the advantage of being a walk-away technology, and the ability to perform a highly standardized platelet function assessment. |
| first_indexed | 2024-03-12T00:27:30Z |
| format | Article |
| id | doaj.art-cbf30194a666472eb1a616f59d9c15b4 |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:27:30Z |
| publishDate | 2017-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-cbf30194a666472eb1a616f59d9c15b42023-09-15T10:31:57ZengTaylor & Francis GroupPlatelets0953-71041369-16352017-10-0128771271910.1080/09537104.2016.12659231265923Evaluation of an automated light transmission aggregometryLi-qin Ling0Juan Liao1Qian Niu2Xia Wang3Jin Jia4Cheng-hua Zuo5Hong Jiang6Jing Zhou7Sichuan UniversityWest China Hospital, Sichuan UniversityWest China Hospital, Sichuan UniversityWest China Hospital, Sichuan UniversityWest China Hospital, Sichuan UniversityWest China Hospital, Sichuan UniversityWest China Hospital, Sichuan UniversityWest China Hospital, Sichuan UniversityLight transmission aggregometry (LTA) is the “gold standard” for platelet function assessment, but it is time-consuming and labor intensive. Recently, an automated platelet aggregation method has been developed on a routine coagulation analyzer (Sysmex CS-2100i). In this study, the performances of CS-2100i including repeatability, correlation with a reference aggregometer (Chrono-log Model 700), and the threshold limitation of platelet counts in platelet-rich plasma (PRP) were evaluated for clinical use. The agonists were adenosine diphosphate (ADP), arachidonic acid, collagen, epinephrine, and ristocetin. The platelet concentration of PRP was adjusted with platelet-poor plasma (PPP) and physiological saline (PS). The CS-2100i showed an excellent repeatability and a strong correlation with the Chrono-log 700 in performing platelet aggregation, and its threshold limitation of platelet counts in PRP was 80 × 109/L. PPP had an inhibitory impact on platelet aggregation induced by ADP, arachidonic acid, collagen or epinephrine; while PS had an inhibitory impact on ristocetin-induced aggregation. PS should be used to adjust PRP for ADP-, arachidonic acid-, collagen-, or epinephrine-induced aggregation; while PPP was recommended for ristocetin-induced aggregation. The CS-2100i showed an excellent repeatability, a strong correlation with Chrono-log 700, a lower platelet count requirement, a shorter turnaround time for samples, the advantage of being a walk-away technology, and the ability to perform a highly standardized platelet function assessment.http://dx.doi.org/10.1080/09537104.2016.1265923automationplatelet aggregationplatelet countplatelet function testsplatelet-rich plasma |
| spellingShingle | Li-qin Ling Juan Liao Qian Niu Xia Wang Jin Jia Cheng-hua Zuo Hong Jiang Jing Zhou Evaluation of an automated light transmission aggregometry Platelets automation platelet aggregation platelet count platelet function tests platelet-rich plasma |
| title | Evaluation of an automated light transmission aggregometry |
| title_full | Evaluation of an automated light transmission aggregometry |
| title_fullStr | Evaluation of an automated light transmission aggregometry |
| title_full_unstemmed | Evaluation of an automated light transmission aggregometry |
| title_short | Evaluation of an automated light transmission aggregometry |
| title_sort | evaluation of an automated light transmission aggregometry |
| topic | automation platelet aggregation platelet count platelet function tests platelet-rich plasma |
| url | http://dx.doi.org/10.1080/09537104.2016.1265923 |
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