Maltose-mediated long-term stabilization of freeze- and spray- dried forms of bovine and porcine hemoglobin
Slaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2019-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2019/0352-51391900067D.pdf |
| _version_ | 1818494714744143872 |
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| author | Drvenica Ivana T. Stančić Ana Z. Kalušević Ana Marković Smilja Dragišić-Maksimović Jelena Nedović Viktor A. Bugarski Branko M. Ilić Vesna Lj. |
| author_facet | Drvenica Ivana T. Stančić Ana Z. Kalušević Ana Marković Smilja Dragišić-Maksimović Jelena Nedović Viktor A. Bugarski Branko M. Ilić Vesna Lj. |
| author_sort | Drvenica Ivana T. |
| collection | DOAJ |
| description | Slaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and storage of bovine and porcine hemoglobin in powders obtained by spray and freeze-drying (without maltose: Hb; with maltose: HbM) were investigated. Differential scanning calorimetry of spray- and freeze-dried powders indicated satisfying quality of the formulation prepared with maltose on dissolving back into solution. After two-year storage at room temperature (20±5°C) in solid forms, protected from moisture and light, rehydrated spray- and freeze-dried HbM were red, while Hb were brown. Dynamic light scattering showed the presence of native hemoglobin monomers in rehydrated spray- and freeze-dried HbM, but their agglomerates in Hb samples. UV–Vis spectrophotometry confirmed an absence of significant hemoglobin denaturation and methemoglobin formation in HbM freeze-dried powders. In spray-dried HbM, an increased level of methemoglobin was detected. The results confirmed the stabilizing effect of maltose, and suggested its use in the production of long-term stable solid-state formulations of hemoglobin, along with drying processes optimization. |
| first_indexed | 2024-12-10T18:10:09Z |
| format | Article |
| id | doaj.art-cbf34e463fcd4f62b6ed2bfe12a423fc |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-12-10T18:10:09Z |
| publishDate | 2019-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-cbf34e463fcd4f62b6ed2bfe12a423fc2022-12-22T01:38:30ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212019-01-0184101105111710.2298/JSC190513067D0352-51391900067DMaltose-mediated long-term stabilization of freeze- and spray- dried forms of bovine and porcine hemoglobinDrvenica Ivana T.0Stančić Ana Z.1Kalušević Ana2Marković Smilja3Dragišić-Maksimović Jelena4Nedović Viktor A.5Bugarski Branko M.6Ilić Vesna Lj.7Institute for Medical Research, University of Belgrade, Belgrade, SerbiaInstitute for Medical Research, University of Belgrade, Belgrade, SerbiaFaculty of Agriculture, University of Belgrade, Belgrade, Serbia + Institute of Meat Hygiene and Technology, Belgrade, Serbia Institute of Technical Sciences of the Serbian Academy of Sciences and Arts, Belgrade, SerbiaInstitute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia Faculty of Agriculture, University of Belgrade, Belgrade, SerbiaFaculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia Institute for Medical Research, University of Belgrade, Belgrade, SerbiaSlaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and storage of bovine and porcine hemoglobin in powders obtained by spray and freeze-drying (without maltose: Hb; with maltose: HbM) were investigated. Differential scanning calorimetry of spray- and freeze-dried powders indicated satisfying quality of the formulation prepared with maltose on dissolving back into solution. After two-year storage at room temperature (20±5°C) in solid forms, protected from moisture and light, rehydrated spray- and freeze-dried HbM were red, while Hb were brown. Dynamic light scattering showed the presence of native hemoglobin monomers in rehydrated spray- and freeze-dried HbM, but their agglomerates in Hb samples. UV–Vis spectrophotometry confirmed an absence of significant hemoglobin denaturation and methemoglobin formation in HbM freeze-dried powders. In spray-dried HbM, an increased level of methemoglobin was detected. The results confirmed the stabilizing effect of maltose, and suggested its use in the production of long-term stable solid-state formulations of hemoglobin, along with drying processes optimization.http://www.doiserbia.nb.rs/img/doi/0352-5139/2019/0352-51391900067D.pdfslaughterhouse bloodheme-iron proteindynamic light scatteringuv-vis spectroscopy |
| spellingShingle | Drvenica Ivana T. Stančić Ana Z. Kalušević Ana Marković Smilja Dragišić-Maksimović Jelena Nedović Viktor A. Bugarski Branko M. Ilić Vesna Lj. Maltose-mediated long-term stabilization of freeze- and spray- dried forms of bovine and porcine hemoglobin Journal of the Serbian Chemical Society slaughterhouse blood heme-iron protein dynamic light scattering uv-vis spectroscopy |
| title | Maltose-mediated long-term stabilization of freeze- and spray- dried forms of bovine and porcine hemoglobin |
| title_full | Maltose-mediated long-term stabilization of freeze- and spray- dried forms of bovine and porcine hemoglobin |
| title_fullStr | Maltose-mediated long-term stabilization of freeze- and spray- dried forms of bovine and porcine hemoglobin |
| title_full_unstemmed | Maltose-mediated long-term stabilization of freeze- and spray- dried forms of bovine and porcine hemoglobin |
| title_short | Maltose-mediated long-term stabilization of freeze- and spray- dried forms of bovine and porcine hemoglobin |
| title_sort | maltose mediated long term stabilization of freeze and spray dried forms of bovine and porcine hemoglobin |
| topic | slaughterhouse blood heme-iron protein dynamic light scattering uv-vis spectroscopy |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2019/0352-51391900067D.pdf |
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