Therapeutic Application of <i>Dendrobium fimbriatum</i> Hook for Retinopathy Caused by Ultraviolet Radiation and Chemotherapy Using ARPE-19 Cells and Mouse Retina
Retinopathy caused by ultraviolet radiation and cancer chemotherapy has increased dramatically in humans due to rapid environmental and social changes. Therefore, it is very important to develop therapeutic strategies to effectively alleviate retinopathy. In China, people often choose dendrobium to...
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MDPI AG
2024-02-01
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| author | Chi-Feng Cheng Sheue-Er Wang Chen-Wen Lu Thi Kim Ngan Nguyen Szu-Chuan Shen Chia-Ying Lien Wu-Chang Chuang Ming-Chung Lee Chung-Hsin Wu |
| author_facet | Chi-Feng Cheng Sheue-Er Wang Chen-Wen Lu Thi Kim Ngan Nguyen Szu-Chuan Shen Chia-Ying Lien Wu-Chang Chuang Ming-Chung Lee Chung-Hsin Wu |
| author_sort | Chi-Feng Cheng |
| collection | DOAJ |
| description | Retinopathy caused by ultraviolet radiation and cancer chemotherapy has increased dramatically in humans due to rapid environmental and social changes. Therefore, it is very important to develop therapeutic strategies to effectively alleviate retinopathy. In China, people often choose dendrobium to improve their eyesight. In this study, we explored how <i>Dendrobium fimbriatum</i> extract (DFE) protects ARPE-19 cells and mouse retinal tissue from damage of ultraviolet (UV) radiation and chemotherapy. We evaluated the antioxidant capacity of DFE using the 1,1-diphenyl-2-trinitophenylhydrazine (DPPH) assay. The protective effects of DEF from UV- and oxaliplatin (OXA)-induced damage were examined in ARPE-19 cells using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and immunofluorescence (IF) stains, and in mouse retinal tissue using immunohistochemistry (IHC) stains. Our results show that DFE has excellent antioxidant capacity. The ARPE-19 cell viability was decreased and the F-actin cytoskeleton structure was damaged by UV radiation and OXA chemotherapy, but both were alleviated after the DFE treatment. Furthermore, DFE treatment can alleviate OXA chemotherapy-induced reduced expressions of rhodopsin and SOD2 and increased expressions of TNF-α and caspase 3 in mouse retinal tissue. Thus, we suggest that DFE can act as suitable treatment for retinopathy through reducing oxidative stress, inflammation, and apoptosis. |
| first_indexed | 2024-04-25T00:22:34Z |
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| language | English |
| last_indexed | 2024-04-25T00:22:34Z |
| publishDate | 2024-02-01 |
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| series | Plants |
| spelling | doaj.art-cbf44f4369814f70a8f92a4e47a5c4882024-03-12T16:52:40ZengMDPI AGPlants2223-77472024-02-0113561710.3390/plants13050617Therapeutic Application of <i>Dendrobium fimbriatum</i> Hook for Retinopathy Caused by Ultraviolet Radiation and Chemotherapy Using ARPE-19 Cells and Mouse RetinaChi-Feng Cheng0Sheue-Er Wang1Chen-Wen Lu2Thi Kim Ngan Nguyen3Szu-Chuan Shen4Chia-Ying Lien5Wu-Chang Chuang6Ming-Chung Lee7Chung-Hsin Wu8School of Life Science, National Taiwan Normal University, Taipei 116, TaiwanDepartment of Pathological Inspection, Saint Paul’s Hospital, Taoyuan 330, TaiwanSchool of Life Science, National Taiwan Normal University, Taipei 116, TaiwanSchool of Life Science, National Taiwan Normal University, Taipei 116, TaiwanSchool of Life Science, National Taiwan Normal University, Taipei 116, TaiwanMaster Program of Sport Facility Management and Health Promotion, National Taiwan University, Taipei 116, TaiwanSun Ten Pharmaceutical Co., Ltd., New Taipei City 231, TaiwanBrion Research Institute of Taiwan, New Taipei City 231, TaiwanSchool of Life Science, National Taiwan Normal University, Taipei 116, TaiwanRetinopathy caused by ultraviolet radiation and cancer chemotherapy has increased dramatically in humans due to rapid environmental and social changes. Therefore, it is very important to develop therapeutic strategies to effectively alleviate retinopathy. In China, people often choose dendrobium to improve their eyesight. In this study, we explored how <i>Dendrobium fimbriatum</i> extract (DFE) protects ARPE-19 cells and mouse retinal tissue from damage of ultraviolet (UV) radiation and chemotherapy. We evaluated the antioxidant capacity of DFE using the 1,1-diphenyl-2-trinitophenylhydrazine (DPPH) assay. The protective effects of DEF from UV- and oxaliplatin (OXA)-induced damage were examined in ARPE-19 cells using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and immunofluorescence (IF) stains, and in mouse retinal tissue using immunohistochemistry (IHC) stains. Our results show that DFE has excellent antioxidant capacity. The ARPE-19 cell viability was decreased and the F-actin cytoskeleton structure was damaged by UV radiation and OXA chemotherapy, but both were alleviated after the DFE treatment. Furthermore, DFE treatment can alleviate OXA chemotherapy-induced reduced expressions of rhodopsin and SOD2 and increased expressions of TNF-α and caspase 3 in mouse retinal tissue. Thus, we suggest that DFE can act as suitable treatment for retinopathy through reducing oxidative stress, inflammation, and apoptosis.https://www.mdpi.com/2223-7747/13/5/617<i>Dendrobium fimbriatum</i>retinopathychemotherapyUVoxidative stressinflammation |
| spellingShingle | Chi-Feng Cheng Sheue-Er Wang Chen-Wen Lu Thi Kim Ngan Nguyen Szu-Chuan Shen Chia-Ying Lien Wu-Chang Chuang Ming-Chung Lee Chung-Hsin Wu Therapeutic Application of <i>Dendrobium fimbriatum</i> Hook for Retinopathy Caused by Ultraviolet Radiation and Chemotherapy Using ARPE-19 Cells and Mouse Retina Plants <i>Dendrobium fimbriatum</i> retinopathy chemotherapy UV oxidative stress inflammation |
| title | Therapeutic Application of <i>Dendrobium fimbriatum</i> Hook for Retinopathy Caused by Ultraviolet Radiation and Chemotherapy Using ARPE-19 Cells and Mouse Retina |
| title_full | Therapeutic Application of <i>Dendrobium fimbriatum</i> Hook for Retinopathy Caused by Ultraviolet Radiation and Chemotherapy Using ARPE-19 Cells and Mouse Retina |
| title_fullStr | Therapeutic Application of <i>Dendrobium fimbriatum</i> Hook for Retinopathy Caused by Ultraviolet Radiation and Chemotherapy Using ARPE-19 Cells and Mouse Retina |
| title_full_unstemmed | Therapeutic Application of <i>Dendrobium fimbriatum</i> Hook for Retinopathy Caused by Ultraviolet Radiation and Chemotherapy Using ARPE-19 Cells and Mouse Retina |
| title_short | Therapeutic Application of <i>Dendrobium fimbriatum</i> Hook for Retinopathy Caused by Ultraviolet Radiation and Chemotherapy Using ARPE-19 Cells and Mouse Retina |
| title_sort | therapeutic application of i dendrobium fimbriatum i hook for retinopathy caused by ultraviolet radiation and chemotherapy using arpe 19 cells and mouse retina |
| topic | <i>Dendrobium fimbriatum</i> retinopathy chemotherapy UV oxidative stress inflammation |
| url | https://www.mdpi.com/2223-7747/13/5/617 |
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