Evaluation of the effect of follicular stimulating hormone on the in vitro bovine spermatogonial stem cells self-renewal: An experimental study
Background: Spermatogonial stem cells (SSCs) are undifferentiated cells which are highly reproducible and expandable. Several studies have been conducted to reproduce these cells in culture. They used growth factors, hormones and different feeder cells to improve survival and proliferation of SSCs....
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| Format: | Article |
| Language: | English |
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Shahid Sadoughi University of Medical Sciences
2017-12-01
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| Series: | International Journal of Reproductive BioMedicine |
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| Online Access: | http://journals.ssu.ac.ir/ijrmnew/browse.php?a_code=A-10-1-364&slc_lang=en&sid=1 |
| _version_ | 1819206702383235072 |
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| author | Masoome Jabarpour |
| author_facet | Masoome Jabarpour |
| author_sort | Masoome Jabarpour |
| collection | DOAJ |
| description | Background: Spermatogonial stem cells (SSCs) are undifferentiated cells which are highly reproducible and expandable. Several studies have been conducted to reproduce these cells in culture. They used growth factors, hormones and different feeder cells to improve survival and proliferation of SSCs. Objective: This study was conducted to evaluate the effects of follicular stimulating hormone (FSH) on gene expression of fibroblast growth factor (FGF2) and glial cell-derived neurotrophic factor (GDNF) in Sertoli cells. Materials and Methods: Sertoli cells and SSCs were isolated from 3-5 month-old calves. Bovine testicular cells were cultured for 15 days with or without FSH. Identification of these cells was confirmed by immunocytochemistry analysis. Colony formation of SSCs was evaluated using an inverted microscope. The gene expression of FGF2 and GDNF and the gene markers bcl6b, thy-1, and C-kit were evaluated using the quantitative RT-PCR technique. Results: The results indicated that FSH increased colonization of SSCs. the expression of GDNF, FGF2, and markers of undifferentiated spermatogonia was increased following culture in control and FSH groups (p<0.05), this increase was more in FSH group. Conversely, the expression of C-kit was decreased in both groups (p<0.05). Conclusion: The results showed that FSH can increase the self-renewal of SSCs in vitro via upregulation of GDNF and FGF2 expression in Sertoli cells. |
| first_indexed | 2024-12-23T05:11:48Z |
| format | Article |
| id | doaj.art-cbf464d61eeb4c6ab675162c16e72382 |
| institution | Directory Open Access Journal |
| issn | 2476-4108 2476-3772 |
| language | English |
| last_indexed | 2024-12-23T05:11:48Z |
| publishDate | 2017-12-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | International Journal of Reproductive BioMedicine |
| spelling | doaj.art-cbf464d61eeb4c6ab675162c16e723822022-12-21T17:58:56ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-41082476-37722017-12-011512795802Evaluation of the effect of follicular stimulating hormone on the in vitro bovine spermatogonial stem cells self-renewal: An experimental studyMasoome Jabarpour0 Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Background: Spermatogonial stem cells (SSCs) are undifferentiated cells which are highly reproducible and expandable. Several studies have been conducted to reproduce these cells in culture. They used growth factors, hormones and different feeder cells to improve survival and proliferation of SSCs. Objective: This study was conducted to evaluate the effects of follicular stimulating hormone (FSH) on gene expression of fibroblast growth factor (FGF2) and glial cell-derived neurotrophic factor (GDNF) in Sertoli cells. Materials and Methods: Sertoli cells and SSCs were isolated from 3-5 month-old calves. Bovine testicular cells were cultured for 15 days with or without FSH. Identification of these cells was confirmed by immunocytochemistry analysis. Colony formation of SSCs was evaluated using an inverted microscope. The gene expression of FGF2 and GDNF and the gene markers bcl6b, thy-1, and C-kit were evaluated using the quantitative RT-PCR technique. Results: The results indicated that FSH increased colonization of SSCs. the expression of GDNF, FGF2, and markers of undifferentiated spermatogonia was increased following culture in control and FSH groups (p<0.05), this increase was more in FSH group. Conversely, the expression of C-kit was decreased in both groups (p<0.05). Conclusion: The results showed that FSH can increase the self-renewal of SSCs in vitro via upregulation of GDNF and FGF2 expression in Sertoli cells.http://journals.ssu.ac.ir/ijrmnew/browse.php?a_code=A-10-1-364&slc_lang=en&sid=1Sertoli cell SSCs FSH Self-renewal |
| spellingShingle | Masoome Jabarpour Evaluation of the effect of follicular stimulating hormone on the in vitro bovine spermatogonial stem cells self-renewal: An experimental study International Journal of Reproductive BioMedicine Sertoli cell SSCs FSH Self-renewal |
| title | Evaluation of the effect of follicular stimulating hormone on the in vitro bovine spermatogonial stem cells self-renewal: An experimental study |
| title_full | Evaluation of the effect of follicular stimulating hormone on the in vitro bovine spermatogonial stem cells self-renewal: An experimental study |
| title_fullStr | Evaluation of the effect of follicular stimulating hormone on the in vitro bovine spermatogonial stem cells self-renewal: An experimental study |
| title_full_unstemmed | Evaluation of the effect of follicular stimulating hormone on the in vitro bovine spermatogonial stem cells self-renewal: An experimental study |
| title_short | Evaluation of the effect of follicular stimulating hormone on the in vitro bovine spermatogonial stem cells self-renewal: An experimental study |
| title_sort | evaluation of the effect of follicular stimulating hormone on the in vitro bovine spermatogonial stem cells self renewal an experimental study |
| topic | Sertoli cell SSCs FSH Self-renewal |
| url | http://journals.ssu.ac.ir/ijrmnew/browse.php?a_code=A-10-1-364&slc_lang=en&sid=1 |
| work_keys_str_mv | AT masoomejabarpour evaluationoftheeffectoffollicularstimulatinghormoneontheinvitrobovinespermatogonialstemcellsselfrenewalanexperimentalstudy |