Searching for New Beneficial Bacterial Isolates of Wild Raspberries for Biocontrol of Phytopathogens-Antagonistic Properties and Functional Characterization
The threat caused by plants fungal and fungal-like pathogens is a serious problem in the organic farming of soft fruits. The European Commission regulations prohibit some commercially available chemical plant protection products, and instead recommend the use of natural methods for improving the mic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/24/9361 |
| _version_ | 1797545397656223744 |
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| author | Michał Pylak Karolina Oszust Magdalena Frąc |
| author_facet | Michał Pylak Karolina Oszust Magdalena Frąc |
| author_sort | Michał Pylak |
| collection | DOAJ |
| description | The threat caused by plants fungal and fungal-like pathogens is a serious problem in the organic farming of soft fruits. The European Commission regulations prohibit some commercially available chemical plant protection products, and instead recommend the use of natural methods for improving the microbial soil status and thus increasing resistance to biotic stresses caused by phytopathogens. The solution to this problem may be biopreparations based on, e.g., bacteria, especially those isolated from native local environments. To select proper bacterial candidates for biopreparation, research was provided to preliminarily ensure that those isolates are able not only to inhibit the growth of pathogens, but also to be metabolically effective. In the presented research sixty-five isolates were acquired and identified. Potentially pathogenic isolates were excluded from further research, and beneficial bacterial isolates were tested against the following plant pathogens: <i>Botrytis</i> spp., <i>Colletotrichum</i> spp., <i>Phytophthora</i> spp., and <i>Verticillium</i> spp. The eight most effective antagonists belonging to <i>Arthrobacter</i>, <i>Bacillus</i>, <i>Pseudomonas</i>, and <i>Rhodococcus</i> genera were subjected to metabolic and enzymatic analyses and a resistance to chemical stress survey, indicating to their potential as components of biopreparations for agroecology. |
| first_indexed | 2024-03-10T14:15:00Z |
| format | Article |
| id | doaj.art-cbfcf31683d4457998c59aa878f6f413 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:15:00Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-cbfcf31683d4457998c59aa878f6f4132023-11-20T23:55:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-12-012124936110.3390/ijms21249361Searching for New Beneficial Bacterial Isolates of Wild Raspberries for Biocontrol of Phytopathogens-Antagonistic Properties and Functional CharacterizationMichał Pylak0Karolina Oszust1Magdalena Frąc2Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, PolandInstitute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, PolandInstitute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, PolandThe threat caused by plants fungal and fungal-like pathogens is a serious problem in the organic farming of soft fruits. The European Commission regulations prohibit some commercially available chemical plant protection products, and instead recommend the use of natural methods for improving the microbial soil status and thus increasing resistance to biotic stresses caused by phytopathogens. The solution to this problem may be biopreparations based on, e.g., bacteria, especially those isolated from native local environments. To select proper bacterial candidates for biopreparation, research was provided to preliminarily ensure that those isolates are able not only to inhibit the growth of pathogens, but also to be metabolically effective. In the presented research sixty-five isolates were acquired and identified. Potentially pathogenic isolates were excluded from further research, and beneficial bacterial isolates were tested against the following plant pathogens: <i>Botrytis</i> spp., <i>Colletotrichum</i> spp., <i>Phytophthora</i> spp., and <i>Verticillium</i> spp. The eight most effective antagonists belonging to <i>Arthrobacter</i>, <i>Bacillus</i>, <i>Pseudomonas</i>, and <i>Rhodococcus</i> genera were subjected to metabolic and enzymatic analyses and a resistance to chemical stress survey, indicating to their potential as components of biopreparations for agroecology.https://www.mdpi.com/1422-0067/21/24/9361beneficial bacteriarhizospheremetabolic analysisphenotypic microarray |
| spellingShingle | Michał Pylak Karolina Oszust Magdalena Frąc Searching for New Beneficial Bacterial Isolates of Wild Raspberries for Biocontrol of Phytopathogens-Antagonistic Properties and Functional Characterization International Journal of Molecular Sciences beneficial bacteria rhizosphere metabolic analysis phenotypic microarray |
| title | Searching for New Beneficial Bacterial Isolates of Wild Raspberries for Biocontrol of Phytopathogens-Antagonistic Properties and Functional Characterization |
| title_full | Searching for New Beneficial Bacterial Isolates of Wild Raspberries for Biocontrol of Phytopathogens-Antagonistic Properties and Functional Characterization |
| title_fullStr | Searching for New Beneficial Bacterial Isolates of Wild Raspberries for Biocontrol of Phytopathogens-Antagonistic Properties and Functional Characterization |
| title_full_unstemmed | Searching for New Beneficial Bacterial Isolates of Wild Raspberries for Biocontrol of Phytopathogens-Antagonistic Properties and Functional Characterization |
| title_short | Searching for New Beneficial Bacterial Isolates of Wild Raspberries for Biocontrol of Phytopathogens-Antagonistic Properties and Functional Characterization |
| title_sort | searching for new beneficial bacterial isolates of wild raspberries for biocontrol of phytopathogens antagonistic properties and functional characterization |
| topic | beneficial bacteria rhizosphere metabolic analysis phenotypic microarray |
| url | https://www.mdpi.com/1422-0067/21/24/9361 |
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