The Development of Cytogenetic Maps for Malaria Mosquitoes
Anopheline mosquitoes are important vectors of human malaria. Next-generation sequencing opens new opportunities for studies of mosquito genomes to uncover the genetic basis of a Plasmodium transmission. Physical mapping of genome sequences to polytene chromosomes significantly improves reference as...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-09-01
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| Series: | Insects |
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| Online Access: | http://www.mdpi.com/2075-4450/9/3/121 |
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| author | Gleb N. Artemov Vladimir N. Stegniy Maria V. Sharakhova Igor V. Sharakhov |
| author_facet | Gleb N. Artemov Vladimir N. Stegniy Maria V. Sharakhova Igor V. Sharakhov |
| author_sort | Gleb N. Artemov |
| collection | DOAJ |
| description | Anopheline mosquitoes are important vectors of human malaria. Next-generation sequencing opens new opportunities for studies of mosquito genomes to uncover the genetic basis of a Plasmodium transmission. Physical mapping of genome sequences to polytene chromosomes significantly improves reference assemblies. High-resolution cytogenetic maps are essential for anchoring genome sequences to chromosomes as well as for studying breakpoints of chromosome rearrangements and chromatin protein localization. Here we describe a detailed pipeline for the development of high-resolution cytogenetic maps using polytene chromosomes of malaria mosquitoes. We apply this workflow to the refinement of the cytogenetic map developed for Anopheles beklemishevi. |
| first_indexed | 2024-12-13T13:57:23Z |
| format | Article |
| id | doaj.art-cbfdd5974c6c403195876c39b789bcbe |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-12-13T13:57:23Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-cbfdd5974c6c403195876c39b789bcbe2022-12-21T23:42:51ZengMDPI AGInsects2075-44502018-09-019312110.3390/insects9030121insects9030121The Development of Cytogenetic Maps for Malaria MosquitoesGleb N. Artemov0Vladimir N. Stegniy1Maria V. Sharakhova2Igor V. Sharakhov3Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State University, 36 Lenin Avenue, 634050 Tomsk, RussiaLaboratory of Ecology, Genetics and Environmental Protection, Tomsk State University, 36 Lenin Avenue, 634050 Tomsk, RussiaLaboratory of Ecology, Genetics and Environmental Protection, Tomsk State University, 36 Lenin Avenue, 634050 Tomsk, RussiaLaboratory of Ecology, Genetics and Environmental Protection, Tomsk State University, 36 Lenin Avenue, 634050 Tomsk, RussiaAnopheline mosquitoes are important vectors of human malaria. Next-generation sequencing opens new opportunities for studies of mosquito genomes to uncover the genetic basis of a Plasmodium transmission. Physical mapping of genome sequences to polytene chromosomes significantly improves reference assemblies. High-resolution cytogenetic maps are essential for anchoring genome sequences to chromosomes as well as for studying breakpoints of chromosome rearrangements and chromatin protein localization. Here we describe a detailed pipeline for the development of high-resolution cytogenetic maps using polytene chromosomes of malaria mosquitoes. We apply this workflow to the refinement of the cytogenetic map developed for Anopheles beklemishevi.http://www.mdpi.com/2075-4450/9/3/121cytogenetic mapchromosome mapmalaria mosquitoesAnophelespolytene chromosomesAnopheles beklemishevichromosome map developmentphotomaphigh-pressure techniquechromosome straightening |
| spellingShingle | Gleb N. Artemov Vladimir N. Stegniy Maria V. Sharakhova Igor V. Sharakhov The Development of Cytogenetic Maps for Malaria Mosquitoes Insects cytogenetic map chromosome map malaria mosquitoes Anopheles polytene chromosomes Anopheles beklemishevi chromosome map development photomap high-pressure technique chromosome straightening |
| title | The Development of Cytogenetic Maps for Malaria Mosquitoes |
| title_full | The Development of Cytogenetic Maps for Malaria Mosquitoes |
| title_fullStr | The Development of Cytogenetic Maps for Malaria Mosquitoes |
| title_full_unstemmed | The Development of Cytogenetic Maps for Malaria Mosquitoes |
| title_short | The Development of Cytogenetic Maps for Malaria Mosquitoes |
| title_sort | development of cytogenetic maps for malaria mosquitoes |
| topic | cytogenetic map chromosome map malaria mosquitoes Anopheles polytene chromosomes Anopheles beklemishevi chromosome map development photomap high-pressure technique chromosome straightening |
| url | http://www.mdpi.com/2075-4450/9/3/121 |
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