CHARACTERISTICS OF PRE-ANALYTICAL STAGE IN IMMUNOPHENOTYPING OF PERIPHERAL BLOOD CELLS
Abstract. Application of flow cytometry (FC) techniques has considerably expanded the opportunities for evaluation of immune cells, aiming for better diagnostics of immunodeficiency disorders, autoimmune and lymphoproliferative diseases, etc.A procedure of laboratory testing is subdivided into sever...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/594 |
| Summary: | Abstract. Application of flow cytometry (FC) techniques has considerably expanded the opportunities for evaluation of immune cells, aiming for better diagnostics of immunodeficiency disorders, autoimmune and lymphoproliferative diseases, etc.A procedure of laboratory testing is subdivided into several stages, i.e., pre-analytical, analytical and post-analytical. At present, some strict criteria are developed for inclusion of various cell parameters into a diagnostic set, along with operational algorithms, in order to optimize the FC procedures. However, the percentage of errors in FC assays still remains high. More than a half of the errors originate from pre-analytical stage.The recommendations listed in this article should definitely influence quality of laboratory investigations and improve diagnostics of immune functional disorders, thus providing adequacy of therapeutic prescriptions. (Med. Immunol., 2011, vol. 13, N 6, pp 639-646) |
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| ISSN: | 1563-0625 2313-741X |