CHARACTERISTICS OF PRE-ANALYTICAL STAGE IN IMMUNOPHENOTYPING OF PERIPHERAL BLOOD CELLS
Abstract. Application of flow cytometry (FC) techniques has considerably expanded the opportunities for evaluation of immune cells, aiming for better diagnostics of immunodeficiency disorders, autoimmune and lymphoproliferative diseases, etc.A procedure of laboratory testing is subdivided into sever...
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/594 |
| _version_ | 1826534286170259456 |
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| author | L. V. Serebrovskaya L. A. Ivanova S. V. Khaidukov |
| author_facet | L. V. Serebrovskaya L. A. Ivanova S. V. Khaidukov |
| author_sort | L. V. Serebrovskaya |
| collection | DOAJ |
| description | Abstract. Application of flow cytometry (FC) techniques has considerably expanded the opportunities for evaluation of immune cells, aiming for better diagnostics of immunodeficiency disorders, autoimmune and lymphoproliferative diseases, etc.A procedure of laboratory testing is subdivided into several stages, i.e., pre-analytical, analytical and post-analytical. At present, some strict criteria are developed for inclusion of various cell parameters into a diagnostic set, along with operational algorithms, in order to optimize the FC procedures. However, the percentage of errors in FC assays still remains high. More than a half of the errors originate from pre-analytical stage.The recommendations listed in this article should definitely influence quality of laboratory investigations and improve diagnostics of immune functional disorders, thus providing adequacy of therapeutic prescriptions. (Med. Immunol., 2011, vol. 13, N 6, pp 639-646) |
| first_indexed | 2024-03-08T05:51:05Z |
| format | Article |
| id | doaj.art-cc008cfeb7c04108a67434bff1b1a453 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2025-03-14T02:20:36Z |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-cc008cfeb7c04108a67434bff1b1a4532025-03-11T17:59:04ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-0113663964610.15789/1563-0625-2011-6-639-646591CHARACTERISTICS OF PRE-ANALYTICAL STAGE IN IMMUNOPHENOTYPING OF PERIPHERAL BLOOD CELLSL. V. Serebrovskaya0L. A. Ivanova1S. V. Khaidukov2ФГУН ЦНИИ Эпидемиологии Роспотребнадзора, МоскваФГУН ЦНИИ Эпидемиологии Роспотребнадзора, МоскваИнститут биоорганической химии им. акад. М.М. Шемякина и Ю.А. Овчинникова РАН, Москва; ФГУ Федеральный Научно-Клинический Центр детской гематологии, онкологии и иммунологии Минздравсоцразвития, МоскваAbstract. Application of flow cytometry (FC) techniques has considerably expanded the opportunities for evaluation of immune cells, aiming for better diagnostics of immunodeficiency disorders, autoimmune and lymphoproliferative diseases, etc.A procedure of laboratory testing is subdivided into several stages, i.e., pre-analytical, analytical and post-analytical. At present, some strict criteria are developed for inclusion of various cell parameters into a diagnostic set, along with operational algorithms, in order to optimize the FC procedures. However, the percentage of errors in FC assays still remains high. More than a half of the errors originate from pre-analytical stage.The recommendations listed in this article should definitely influence quality of laboratory investigations and improve diagnostics of immune functional disorders, thus providing adequacy of therapeutic prescriptions. (Med. Immunol., 2011, vol. 13, N 6, pp 639-646)https://www.mimmun.ru/mimmun/article/view/594flow cytometryimmunophenotypingpre-analytical stage |
| spellingShingle | L. V. Serebrovskaya L. A. Ivanova S. V. Khaidukov CHARACTERISTICS OF PRE-ANALYTICAL STAGE IN IMMUNOPHENOTYPING OF PERIPHERAL BLOOD CELLS Медицинская иммунология flow cytometry immunophenotyping pre-analytical stage |
| title | CHARACTERISTICS OF PRE-ANALYTICAL STAGE IN IMMUNOPHENOTYPING OF PERIPHERAL BLOOD CELLS |
| title_full | CHARACTERISTICS OF PRE-ANALYTICAL STAGE IN IMMUNOPHENOTYPING OF PERIPHERAL BLOOD CELLS |
| title_fullStr | CHARACTERISTICS OF PRE-ANALYTICAL STAGE IN IMMUNOPHENOTYPING OF PERIPHERAL BLOOD CELLS |
| title_full_unstemmed | CHARACTERISTICS OF PRE-ANALYTICAL STAGE IN IMMUNOPHENOTYPING OF PERIPHERAL BLOOD CELLS |
| title_short | CHARACTERISTICS OF PRE-ANALYTICAL STAGE IN IMMUNOPHENOTYPING OF PERIPHERAL BLOOD CELLS |
| title_sort | characteristics of pre analytical stage in immunophenotyping of peripheral blood cells |
| topic | flow cytometry immunophenotyping pre-analytical stage |
| url | https://www.mimmun.ru/mimmun/article/view/594 |
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