Ameliorative effect of pumpkin oil (Cucurbita pepo L.) against alcohol-induced hepatotoxicity and oxidative stress in albino rats
Objective: The aim of the present study was to evaluate the protective role of pumpkin oil on experimental alcohol – induced hepatotoxicity. Materials and methods: Rats are divided into three groups of 10 animals each. Group one (G1) was the control group is orally given distilled water for 4 weeks....
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| Format: | Article |
| Language: | English |
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SpringerOpen
2014-09-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2314853514000523 |
| _version_ | 1818190439385137152 |
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| author | Howida Sayed Abou Seif |
| author_facet | Howida Sayed Abou Seif |
| author_sort | Howida Sayed Abou Seif |
| collection | DOAJ |
| description | Objective: The aim of the present study was to evaluate the protective role of pumpkin oil on experimental alcohol – induced hepatotoxicity.
Materials and methods: Rats are divided into three groups of 10 animals each. Group one (G1) was the control group is orally given distilled water for 4 weeks. Group two (G2) is given absolute ethyl alcohol (10%) in drinking water for 4 weeks. Group three (G3) alcohol– administered rats were pretreated with pumpkin oil (50 mg/kg body weight) three times per week for three weeks and alcohol (10%) three times per week (at the first two weeks of the experiment).
Results: Alcohol caused a marked rise in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and gamma glutamyl transferase (γGT) activities. Concerning oxidative stress and antioxidant defense system, the depleted hepatic glutathione content, glutathione-S-transferase and catalase activities of alcohol-administered rats were potentially increased above normal levels as a result of pretreatment with pumpkin oil. However, while elevated lipid peroxidation was noticed in alcohol treated rats, pretreatment with pumpkin oil produced a detectable decrease in lipid peroxidation level.
Conclusion: The natural plant components found in pumpkin could improve the liver against alcohol-induced liver toxicity and oxidative stress. However, further clinical studies are required to assess the safety and benefits of pumpkin oil in human beings. |
| first_indexed | 2024-12-11T23:58:44Z |
| format | Article |
| id | doaj.art-cc016984181047989a7d2f2b9f69aa0c |
| institution | Directory Open Access Journal |
| issn | 2314-8535 |
| language | English |
| last_indexed | 2024-12-11T23:58:44Z |
| publishDate | 2014-09-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-cc016984181047989a7d2f2b9f69aa0c2022-12-22T00:45:17ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85352014-09-013317818510.1016/j.bjbas.2014.08.001Ameliorative effect of pumpkin oil (Cucurbita pepo L.) against alcohol-induced hepatotoxicity and oxidative stress in albino ratsHowida Sayed Abou SeifObjective: The aim of the present study was to evaluate the protective role of pumpkin oil on experimental alcohol – induced hepatotoxicity. Materials and methods: Rats are divided into three groups of 10 animals each. Group one (G1) was the control group is orally given distilled water for 4 weeks. Group two (G2) is given absolute ethyl alcohol (10%) in drinking water for 4 weeks. Group three (G3) alcohol– administered rats were pretreated with pumpkin oil (50 mg/kg body weight) three times per week for three weeks and alcohol (10%) three times per week (at the first two weeks of the experiment). Results: Alcohol caused a marked rise in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and gamma glutamyl transferase (γGT) activities. Concerning oxidative stress and antioxidant defense system, the depleted hepatic glutathione content, glutathione-S-transferase and catalase activities of alcohol-administered rats were potentially increased above normal levels as a result of pretreatment with pumpkin oil. However, while elevated lipid peroxidation was noticed in alcohol treated rats, pretreatment with pumpkin oil produced a detectable decrease in lipid peroxidation level. Conclusion: The natural plant components found in pumpkin could improve the liver against alcohol-induced liver toxicity and oxidative stress. However, further clinical studies are required to assess the safety and benefits of pumpkin oil in human beings.http://www.sciencedirect.com/science/article/pii/S2314853514000523Pumpkin oil-hepatotoxicityRattus norvegicusOxidative stress |
| spellingShingle | Howida Sayed Abou Seif Ameliorative effect of pumpkin oil (Cucurbita pepo L.) against alcohol-induced hepatotoxicity and oxidative stress in albino rats Beni-Suef University Journal of Basic and Applied Sciences Pumpkin oil-hepatotoxicity Rattus norvegicus Oxidative stress |
| title | Ameliorative effect of pumpkin oil (Cucurbita pepo L.) against alcohol-induced hepatotoxicity and oxidative stress in albino rats |
| title_full | Ameliorative effect of pumpkin oil (Cucurbita pepo L.) against alcohol-induced hepatotoxicity and oxidative stress in albino rats |
| title_fullStr | Ameliorative effect of pumpkin oil (Cucurbita pepo L.) against alcohol-induced hepatotoxicity and oxidative stress in albino rats |
| title_full_unstemmed | Ameliorative effect of pumpkin oil (Cucurbita pepo L.) against alcohol-induced hepatotoxicity and oxidative stress in albino rats |
| title_short | Ameliorative effect of pumpkin oil (Cucurbita pepo L.) against alcohol-induced hepatotoxicity and oxidative stress in albino rats |
| title_sort | ameliorative effect of pumpkin oil cucurbita pepo l against alcohol induced hepatotoxicity and oxidative stress in albino rats |
| topic | Pumpkin oil-hepatotoxicity Rattus norvegicus Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S2314853514000523 |
| work_keys_str_mv | AT howidasayedabouseif ameliorativeeffectofpumpkinoilcucurbitapepolagainstalcoholinducedhepatotoxicityandoxidativestressinalbinorats |