Reliability of rubidium-82 PET/CT for renal perfusion determination in healthy subjects
Abstract Background Changes in renal perfusion may play a pathophysiological role in hypertension and kidney disease, however to date, no method for renal blood flow (RBF) determination in humans has been implemented in clinical practice. In a previous study, we demonstrated that estimation of renal...
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Nephrology |
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| Online Access: | https://doi.org/10.1186/s12882-022-02962-w |
| _version_ | 1811207632420077568 |
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| author | Stine Sundgaard Langaa Frank Holden Mose Claire Anne Fynbo Jørn Theil Jesper Nørgaard Bech |
| author_facet | Stine Sundgaard Langaa Frank Holden Mose Claire Anne Fynbo Jørn Theil Jesper Nørgaard Bech |
| author_sort | Stine Sundgaard Langaa |
| collection | DOAJ |
| description | Abstract Background Changes in renal perfusion may play a pathophysiological role in hypertension and kidney disease, however to date, no method for renal blood flow (RBF) determination in humans has been implemented in clinical practice. In a previous study, we demonstrated that estimation of renal perfusion based on a single positron emission tomography/computed tomography (PET/CT) scan with Rubidium-82 (82Rb) is feasible and found an approximate 5% intra-assay coefficient of variation for both kidneys, indicative of a precise method.This study’s aim was to determine the day-to day variation of 82Rb PET/CT and to test the method’s ability to detect increased RBF induced by infusion of amino acids. Methods Seventeen healthy subjects underwent three dynamic 82Rb PET/CT scans over two examination days comprising: Day A, a single 8-minute dynamic scan and Day B, two scans performed before (baseline) and after RBF stimulation by a 2-hour amino acid-infusion. The order of examination days was determined by randomization. Time activity curves for arterial and renal activity with a 1-tissue compartment model were used for flow estimation; the K1 kinetic parameter representing renal 82Rb clearance. Day-to-day variation was calculated based on the difference between the unstimulated K1 values on Day A and Day B and paired t-testing was performed to compare K1 values at baseline and after RBF stimulation on Day B. Results Day-to-day variation was observed to be 5.5% for the right kidney and 6.0% for the left kidney (n = 15 quality accepted scans). K1 values determined after amino acid-infusion were significantly higher than pre-infusion values (n = 17, p = 0.001). The mean percentage change in K1 from baseline was 13.2 ± 12.9% (range − 10.4 to 35.5) for the right kidney; 12.9 ± 13.2% (range − 15.7 to 35.3) for the left kidney. Conclusion Day-to-day variation is acceptably low. A significant K1 increase from baseline is detected after application of a known RBF stimulus, indicating that 82Rb PET/CT scanning can provide a precise method for evaluation of RBF and it is able to determine changes herein. Clinical Trial Registration EU Clinical Trials Register, 2017-005008-88. Registered 18/01/2018. |
| first_indexed | 2024-04-12T04:07:57Z |
| format | Article |
| id | doaj.art-cc0397fcc04d4b9cbffab222abd36199 |
| institution | Directory Open Access Journal |
| issn | 1471-2369 |
| language | English |
| last_indexed | 2024-04-12T04:07:57Z |
| publishDate | 2022-11-01 |
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| series | BMC Nephrology |
| spelling | doaj.art-cc0397fcc04d4b9cbffab222abd361992022-12-22T03:48:35ZengBMCBMC Nephrology1471-23692022-11-0123111010.1186/s12882-022-02962-wReliability of rubidium-82 PET/CT for renal perfusion determination in healthy subjectsStine Sundgaard Langaa0Frank Holden Mose1Claire Anne Fynbo2Jørn Theil3Jesper Nørgaard Bech4University Clinic in Nephrology and Hypertension, Department of Medicine, Gødstrup Hospital and Aarhus UniversityUniversity Clinic in Nephrology and Hypertension, Department of Medicine, Gødstrup Hospital and Aarhus UniversityClinic for Nuclear Medicine, Gødstrup HospitalClinic for Nuclear Medicine, Gødstrup HospitalUniversity Clinic in Nephrology and Hypertension, Department of Medicine, Gødstrup Hospital and Aarhus UniversityAbstract Background Changes in renal perfusion may play a pathophysiological role in hypertension and kidney disease, however to date, no method for renal blood flow (RBF) determination in humans has been implemented in clinical practice. In a previous study, we demonstrated that estimation of renal perfusion based on a single positron emission tomography/computed tomography (PET/CT) scan with Rubidium-82 (82Rb) is feasible and found an approximate 5% intra-assay coefficient of variation for both kidneys, indicative of a precise method.This study’s aim was to determine the day-to day variation of 82Rb PET/CT and to test the method’s ability to detect increased RBF induced by infusion of amino acids. Methods Seventeen healthy subjects underwent three dynamic 82Rb PET/CT scans over two examination days comprising: Day A, a single 8-minute dynamic scan and Day B, two scans performed before (baseline) and after RBF stimulation by a 2-hour amino acid-infusion. The order of examination days was determined by randomization. Time activity curves for arterial and renal activity with a 1-tissue compartment model were used for flow estimation; the K1 kinetic parameter representing renal 82Rb clearance. Day-to-day variation was calculated based on the difference between the unstimulated K1 values on Day A and Day B and paired t-testing was performed to compare K1 values at baseline and after RBF stimulation on Day B. Results Day-to-day variation was observed to be 5.5% for the right kidney and 6.0% for the left kidney (n = 15 quality accepted scans). K1 values determined after amino acid-infusion were significantly higher than pre-infusion values (n = 17, p = 0.001). The mean percentage change in K1 from baseline was 13.2 ± 12.9% (range − 10.4 to 35.5) for the right kidney; 12.9 ± 13.2% (range − 15.7 to 35.3) for the left kidney. Conclusion Day-to-day variation is acceptably low. A significant K1 increase from baseline is detected after application of a known RBF stimulus, indicating that 82Rb PET/CT scanning can provide a precise method for evaluation of RBF and it is able to determine changes herein. Clinical Trial Registration EU Clinical Trials Register, 2017-005008-88. Registered 18/01/2018.https://doi.org/10.1186/s12882-022-02962-wRubidium-82PET/CTPharmacokinetic modellingRenal clearanceRenal blood FlowRenal plasma flow |
| spellingShingle | Stine Sundgaard Langaa Frank Holden Mose Claire Anne Fynbo Jørn Theil Jesper Nørgaard Bech Reliability of rubidium-82 PET/CT for renal perfusion determination in healthy subjects BMC Nephrology Rubidium-82 PET/CT Pharmacokinetic modelling Renal clearance Renal blood Flow Renal plasma flow |
| title | Reliability of rubidium-82 PET/CT for renal perfusion determination in healthy subjects |
| title_full | Reliability of rubidium-82 PET/CT for renal perfusion determination in healthy subjects |
| title_fullStr | Reliability of rubidium-82 PET/CT for renal perfusion determination in healthy subjects |
| title_full_unstemmed | Reliability of rubidium-82 PET/CT for renal perfusion determination in healthy subjects |
| title_short | Reliability of rubidium-82 PET/CT for renal perfusion determination in healthy subjects |
| title_sort | reliability of rubidium 82 pet ct for renal perfusion determination in healthy subjects |
| topic | Rubidium-82 PET/CT Pharmacokinetic modelling Renal clearance Renal blood Flow Renal plasma flow |
| url | https://doi.org/10.1186/s12882-022-02962-w |
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