Dietary Protein Level Influences on Neurotensin-immunoreactive Cells in the Chicken Ileum
Neurotensin is secreted from intestinal N cells in response to the food ingestion. Influences of different dietary protein levels on neurotensin-immunoreactive cells in the chicken ileum were examined by using immunohistochemical and morphometrical techniques. The results showed that dietary protein...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2020-10-01
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| Series: | The Journal of Poultry Science |
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| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/57/4/57_0190132/_html/-char/en |
| _version_ | 1797817082910343168 |
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| author | Md Salahuddin Kohzy Hiramatsu Kazumi Kita |
| author_facet | Md Salahuddin Kohzy Hiramatsu Kazumi Kita |
| author_sort | Md Salahuddin |
| collection | DOAJ |
| description | Neurotensin is secreted from intestinal N cells in response to the food ingestion. Influences of different dietary protein levels on neurotensin-immunoreactive cells in the chicken ileum were examined by using immunohistochemical and morphometrical techniques. The results showed that dietary protein had an obvious influence on neurotensin-immunoreactive cells in the chicken ileum. Four experimental groups were used, with dietary crude protein (CP) levels of 18% (control), 9%, 4.5% and 0%. Enteroendocrine cells showing neurotensin-immunoreactivity were located in crypts and villous epithelium in all groups. Most of the neurotensin-immunoreactive cells in the villous epithelium showed pyramidal or spindle-like shape with a long cytoplasmic process reaching the intestinal lumen, but cells with round or oval shape were found in the CP4.5% and 0% groups. Frequencies of occurrence of neurotensin-immunoreactive cells in the CP18%, 9%, 4.5% and 0% groups were 42.4±3.3, 36.6±2.2, 30.8±2.6 and 25.4±3.8 (cell count per mucosal area: cells/mm2, mean±SD), respectively. There were significant differences in neurotensin-immunoreactive cell frequency between the control and lower CP level, 4.5% and 0%, groups. A significant correlation was found between frequency of occurrence of neurotensin-immunoreactive cells and daily protein intake. These results indicate that ingested protein is likely to be a potential signal for neurotensin production and secretion of N cells in the chicken ileum. |
| first_indexed | 2024-03-13T08:47:26Z |
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| id | doaj.art-cc045df6228c43a2b58c9a7305a696d9 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:47:26Z |
| publishDate | 2020-10-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-cc045df6228c43a2b58c9a7305a696d92023-05-30T01:08:01ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862020-10-0157429730210.2141/jpsa.0190132jpsaDietary Protein Level Influences on Neurotensin-immunoreactive Cells in the Chicken IleumMd Salahuddin0Kohzy Hiramatsu1Kazumi Kita2Department of Science and Technology, Graduate School of Medicine, Science and Technology, Shinshu UniversityLaboratory of Animal Functional Anatomy (LAFA), Faculty of Agriculture, Shinshu UniversityLaboratory of Animal Nutrition, Faculty of Agriculture, Iwate UniversityNeurotensin is secreted from intestinal N cells in response to the food ingestion. Influences of different dietary protein levels on neurotensin-immunoreactive cells in the chicken ileum were examined by using immunohistochemical and morphometrical techniques. The results showed that dietary protein had an obvious influence on neurotensin-immunoreactive cells in the chicken ileum. Four experimental groups were used, with dietary crude protein (CP) levels of 18% (control), 9%, 4.5% and 0%. Enteroendocrine cells showing neurotensin-immunoreactivity were located in crypts and villous epithelium in all groups. Most of the neurotensin-immunoreactive cells in the villous epithelium showed pyramidal or spindle-like shape with a long cytoplasmic process reaching the intestinal lumen, but cells with round or oval shape were found in the CP4.5% and 0% groups. Frequencies of occurrence of neurotensin-immunoreactive cells in the CP18%, 9%, 4.5% and 0% groups were 42.4±3.3, 36.6±2.2, 30.8±2.6 and 25.4±3.8 (cell count per mucosal area: cells/mm2, mean±SD), respectively. There were significant differences in neurotensin-immunoreactive cell frequency between the control and lower CP level, 4.5% and 0%, groups. A significant correlation was found between frequency of occurrence of neurotensin-immunoreactive cells and daily protein intake. These results indicate that ingested protein is likely to be a potential signal for neurotensin production and secretion of N cells in the chicken ileum.https://www.jstage.jst.go.jp/article/jpsa/57/4/57_0190132/_html/-char/enchickendietary proteinileumimmunohistochemistryneurotensin |
| spellingShingle | Md Salahuddin Kohzy Hiramatsu Kazumi Kita Dietary Protein Level Influences on Neurotensin-immunoreactive Cells in the Chicken Ileum The Journal of Poultry Science chicken dietary protein ileum immunohistochemistry neurotensin |
| title | Dietary Protein Level Influences on Neurotensin-immunoreactive Cells in the Chicken Ileum |
| title_full | Dietary Protein Level Influences on Neurotensin-immunoreactive Cells in the Chicken Ileum |
| title_fullStr | Dietary Protein Level Influences on Neurotensin-immunoreactive Cells in the Chicken Ileum |
| title_full_unstemmed | Dietary Protein Level Influences on Neurotensin-immunoreactive Cells in the Chicken Ileum |
| title_short | Dietary Protein Level Influences on Neurotensin-immunoreactive Cells in the Chicken Ileum |
| title_sort | dietary protein level influences on neurotensin immunoreactive cells in the chicken ileum |
| topic | chicken dietary protein ileum immunohistochemistry neurotensin |
| url | https://www.jstage.jst.go.jp/article/jpsa/57/4/57_0190132/_html/-char/en |
| work_keys_str_mv | AT mdsalahuddin dietaryproteinlevelinfluencesonneurotensinimmunoreactivecellsinthechickenileum AT kohzyhiramatsu dietaryproteinlevelinfluencesonneurotensinimmunoreactivecellsinthechickenileum AT kazumikita dietaryproteinlevelinfluencesonneurotensinimmunoreactivecellsinthechickenileum |