Possible anti-inflammatory role of Zingiberis processum rhizoma, one component of the Kampo formula daikenchuto, against neutrophil infiltration through muscarinic acetylcholine receptor activation
Zingiberis processum rhizoma (ZPR) is a major active component of daikenchuto (DKT), which induces anti-inflammatory action by inhibiting macrophage infiltration. However, it is unclear whether ZPR is related to DKT-induced anti-inflammatory action via a reduction of neutrophil infiltration against...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-08-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861318301324 |
| _version_ | 1811259246105329664 |
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| author | Mari Endo Masatoshi Hori Hiroshi Ozaki Tetsuro Oikawa Hiroshi Odaguchi Toshihiko Hanawa |
| author_facet | Mari Endo Masatoshi Hori Hiroshi Ozaki Tetsuro Oikawa Hiroshi Odaguchi Toshihiko Hanawa |
| author_sort | Mari Endo |
| collection | DOAJ |
| description | Zingiberis processum rhizoma (ZPR) is a major active component of daikenchuto (DKT), which induces anti-inflammatory action by inhibiting macrophage infiltration. However, it is unclear whether ZPR is related to DKT-induced anti-inflammatory action via a reduction of neutrophil infiltration against postoperative ileus (POI). In this study, we orally administered individual herbal components of DKT to mice four times before and after intestinal manipulation (IM). The anti-inflammatory action of each crude drug was evaluated by histochemical analysis of relevant molecules. The results showed that treatment with all herbal components of DKT significantly inhibits neutrophil infiltration. This inhibition of neutrophil infiltration by ZPR was significantly reduced in 5-hydroxytryptamine receptor 4 (5-HT4R) knockout (KO) mice but not in alpha-7 nicotinic acetylcholine receptor (α7nAChR) KO mice. Also, transient receptor potential ankyrin 1 (TRPA1) and muscarinic acetylcholine receptor (mAChR) antagonists partly and significantly inhibited the amelioration of neutrophil infiltration by ZPR. Therefore, DKT-induced anti-inflammatory action, mediated by inhibition of neutrophil infiltration in POI, depends, in part, on the effects of ZPR. ZPR activates TRPA1 channels, possibly in enterochromaffin (EC) cells, to release 5-HT. This 5-HT stimulates 5-HT4R in the myenteric plexus neurons to release acetylcholine, which, in turn, activates mAChR to inhibit inflammation in POI. Keywords: Anti-inflammatory activity, Postoperative ileus, Zingiberis processum rhizoma, Neutrophil infiltration, Daikenchuto |
| first_indexed | 2024-04-12T18:27:34Z |
| format | Article |
| id | doaj.art-cc0494104917433aa0809ca5c8c43350 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-04-12T18:27:34Z |
| publishDate | 2018-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-cc0494104917433aa0809ca5c8c433502022-12-22T03:21:11ZengElsevierJournal of Pharmacological Sciences1347-86132018-08-011374379386Possible anti-inflammatory role of Zingiberis processum rhizoma, one component of the Kampo formula daikenchuto, against neutrophil infiltration through muscarinic acetylcholine receptor activationMari Endo0Masatoshi Hori1Hiroshi Ozaki2Tetsuro Oikawa3Hiroshi Odaguchi4Toshihiko Hanawa5Department of Clinical Research, Oriental Medicine Research Center, Kitasato University, Japan; Corresponding author. 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8642, Japan. Fax: +81 3 5791 6171.Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Japan; Corresponding author. 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan. Fax: +81 3 5841 8183.Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, JapanDepartment of Clinical Research, Oriental Medicine Research Center, Kitasato University, JapanDepartment of Clinical Research, Oriental Medicine Research Center, Kitasato University, JapanDepartment of Clinical Research, Oriental Medicine Research Center, Kitasato University, Japan; Department of Oriental Medicine Research, Research and Development Center for Medical Education, Kitasato University School of Medicine, Japan; Department of Oriental Medicine, Doctoral Program of Medical Science, Kitasato University Graduate School, JapanZingiberis processum rhizoma (ZPR) is a major active component of daikenchuto (DKT), which induces anti-inflammatory action by inhibiting macrophage infiltration. However, it is unclear whether ZPR is related to DKT-induced anti-inflammatory action via a reduction of neutrophil infiltration against postoperative ileus (POI). In this study, we orally administered individual herbal components of DKT to mice four times before and after intestinal manipulation (IM). The anti-inflammatory action of each crude drug was evaluated by histochemical analysis of relevant molecules. The results showed that treatment with all herbal components of DKT significantly inhibits neutrophil infiltration. This inhibition of neutrophil infiltration by ZPR was significantly reduced in 5-hydroxytryptamine receptor 4 (5-HT4R) knockout (KO) mice but not in alpha-7 nicotinic acetylcholine receptor (α7nAChR) KO mice. Also, transient receptor potential ankyrin 1 (TRPA1) and muscarinic acetylcholine receptor (mAChR) antagonists partly and significantly inhibited the amelioration of neutrophil infiltration by ZPR. Therefore, DKT-induced anti-inflammatory action, mediated by inhibition of neutrophil infiltration in POI, depends, in part, on the effects of ZPR. ZPR activates TRPA1 channels, possibly in enterochromaffin (EC) cells, to release 5-HT. This 5-HT stimulates 5-HT4R in the myenteric plexus neurons to release acetylcholine, which, in turn, activates mAChR to inhibit inflammation in POI. Keywords: Anti-inflammatory activity, Postoperative ileus, Zingiberis processum rhizoma, Neutrophil infiltration, Daikenchutohttp://www.sciencedirect.com/science/article/pii/S1347861318301324 |
| spellingShingle | Mari Endo Masatoshi Hori Hiroshi Ozaki Tetsuro Oikawa Hiroshi Odaguchi Toshihiko Hanawa Possible anti-inflammatory role of Zingiberis processum rhizoma, one component of the Kampo formula daikenchuto, against neutrophil infiltration through muscarinic acetylcholine receptor activation Journal of Pharmacological Sciences |
| title | Possible anti-inflammatory role of Zingiberis processum rhizoma, one component of the Kampo formula daikenchuto, against neutrophil infiltration through muscarinic acetylcholine receptor activation |
| title_full | Possible anti-inflammatory role of Zingiberis processum rhizoma, one component of the Kampo formula daikenchuto, against neutrophil infiltration through muscarinic acetylcholine receptor activation |
| title_fullStr | Possible anti-inflammatory role of Zingiberis processum rhizoma, one component of the Kampo formula daikenchuto, against neutrophil infiltration through muscarinic acetylcholine receptor activation |
| title_full_unstemmed | Possible anti-inflammatory role of Zingiberis processum rhizoma, one component of the Kampo formula daikenchuto, against neutrophil infiltration through muscarinic acetylcholine receptor activation |
| title_short | Possible anti-inflammatory role of Zingiberis processum rhizoma, one component of the Kampo formula daikenchuto, against neutrophil infiltration through muscarinic acetylcholine receptor activation |
| title_sort | possible anti inflammatory role of zingiberis processum rhizoma one component of the kampo formula daikenchuto against neutrophil infiltration through muscarinic acetylcholine receptor activation |
| url | http://www.sciencedirect.com/science/article/pii/S1347861318301324 |
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