Enhanced cytotoxicity of auraptene to prostate cancer cells by dextran-coated Fe3O4 nanoparticles
Purpose: Auraptene (AUR) is a monoterpene coumarin compound with several biological activities specifically anti-cancer. The bioavailability of AUR in biological fluids is negligible, thus, the cytotoxicity of this compound for the target cells is low. Herein, the synthesis of AUR-coated Fe3O4 nanop...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2022-01-01
|
| Series: | Nanomedicine Journal |
| Subjects: | |
| Online Access: | https://nmj.mums.ac.ir/article_19284_bb45a313c30da9f2ac3b7ba9208dcf8f.pdf |
| _version_ | 1819095156032274432 |
|---|---|
| author | Narges Naseri Mehrdad Iranshahi zahra Tayarani-Najaran Saleh Rakhshani Leila Mohtashami |
| author_facet | Narges Naseri Mehrdad Iranshahi zahra Tayarani-Najaran Saleh Rakhshani Leila Mohtashami |
| author_sort | Narges Naseri |
| collection | DOAJ |
| description | Purpose: Auraptene (AUR) is a monoterpene coumarin compound with several biological activities specifically anti-cancer. The bioavailability of AUR in biological fluids is negligible, thus, the cytotoxicity of this compound for the target cells is low. Herein, the synthesis of AUR-coated Fe3O4 nanoparticles is presented as a strategy to increase the cytotoxicity of AUR on PC3, DU145, and LNCaP prostate cancer cells.Methods: Fe3O4 nanoparticles were synthesized via co-precipitation method, coated with AUR and stabilized by dextran. They were characterized by X-ray diffraction spectroscopy (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic light scattering (DLS) analysis, and vibrating sample magnetometry (VSM). In vitro release test for coated nanoparticles was performed in both physiologic (pH= 7.4) and acidic (pH= 5.5) environments. Cytotoxicity for prostate cancer cells was evaluated by AlamarBlue assay and the results were analyzed by one-way and two-way ANOVA tests.Results: Characterization outcomes represented the formation of magnetic nanoparticles with good crystalline structure, relatively spherical shape and superparamagnetic properties. AUR release profile from nanoparticles demonstrated that coated nanoparticles are able to inhibit burst release of this compound. AUR release was remarkably higher in acidic medium that can be advantageous for treating tumor regions. Cytotoxicity results indicated that AUR had a very low toxicity against prostate cancer cells at the tested concentrations. In contrast, AUR-coated Fe3O4 nanoparticles were significantly cytotoxic on all the cell lines.Conclusion: The coating of AUR on the surface of Fe3O4 nanoparticles was a successful approach to enhance the efficacy and cytotoxicity of this compound. |
| first_indexed | 2024-12-21T23:38:49Z |
| format | Article |
| id | doaj.art-cc05def9b4c84a00ae626ee78e230e10 |
| institution | Directory Open Access Journal |
| issn | 2322-3049 2322-5904 |
| language | English |
| last_indexed | 2024-12-21T23:38:49Z |
| publishDate | 2022-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Nanomedicine Journal |
| spelling | doaj.art-cc05def9b4c84a00ae626ee78e230e102022-12-21T18:46:17ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042022-01-0191778610.22038/nmj.2021.61479.163319284Enhanced cytotoxicity of auraptene to prostate cancer cells by dextran-coated Fe3O4 nanoparticlesNarges Naseri0Mehrdad Iranshahi1zahra Tayarani-Najaran2Saleh Rakhshani3Leila Mohtashami4Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranBiotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranTargeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranPurpose: Auraptene (AUR) is a monoterpene coumarin compound with several biological activities specifically anti-cancer. The bioavailability of AUR in biological fluids is negligible, thus, the cytotoxicity of this compound for the target cells is low. Herein, the synthesis of AUR-coated Fe3O4 nanoparticles is presented as a strategy to increase the cytotoxicity of AUR on PC3, DU145, and LNCaP prostate cancer cells.Methods: Fe3O4 nanoparticles were synthesized via co-precipitation method, coated with AUR and stabilized by dextran. They were characterized by X-ray diffraction spectroscopy (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic light scattering (DLS) analysis, and vibrating sample magnetometry (VSM). In vitro release test for coated nanoparticles was performed in both physiologic (pH= 7.4) and acidic (pH= 5.5) environments. Cytotoxicity for prostate cancer cells was evaluated by AlamarBlue assay and the results were analyzed by one-way and two-way ANOVA tests.Results: Characterization outcomes represented the formation of magnetic nanoparticles with good crystalline structure, relatively spherical shape and superparamagnetic properties. AUR release profile from nanoparticles demonstrated that coated nanoparticles are able to inhibit burst release of this compound. AUR release was remarkably higher in acidic medium that can be advantageous for treating tumor regions. Cytotoxicity results indicated that AUR had a very low toxicity against prostate cancer cells at the tested concentrations. In contrast, AUR-coated Fe3O4 nanoparticles were significantly cytotoxic on all the cell lines.Conclusion: The coating of AUR on the surface of Fe3O4 nanoparticles was a successful approach to enhance the efficacy and cytotoxicity of this compound.https://nmj.mums.ac.ir/article_19284_bb45a313c30da9f2ac3b7ba9208dcf8f.pdfauraptenecoumarincytotoxicityfe3o4 nanoparticlesprostate cancer |
| spellingShingle | Narges Naseri Mehrdad Iranshahi zahra Tayarani-Najaran Saleh Rakhshani Leila Mohtashami Enhanced cytotoxicity of auraptene to prostate cancer cells by dextran-coated Fe3O4 nanoparticles Nanomedicine Journal auraptene coumarin cytotoxicity fe3o4 nanoparticles prostate cancer |
| title | Enhanced cytotoxicity of auraptene to prostate cancer cells by dextran-coated Fe3O4 nanoparticles |
| title_full | Enhanced cytotoxicity of auraptene to prostate cancer cells by dextran-coated Fe3O4 nanoparticles |
| title_fullStr | Enhanced cytotoxicity of auraptene to prostate cancer cells by dextran-coated Fe3O4 nanoparticles |
| title_full_unstemmed | Enhanced cytotoxicity of auraptene to prostate cancer cells by dextran-coated Fe3O4 nanoparticles |
| title_short | Enhanced cytotoxicity of auraptene to prostate cancer cells by dextran-coated Fe3O4 nanoparticles |
| title_sort | enhanced cytotoxicity of auraptene to prostate cancer cells by dextran coated fe3o4 nanoparticles |
| topic | auraptene coumarin cytotoxicity fe3o4 nanoparticles prostate cancer |
| url | https://nmj.mums.ac.ir/article_19284_bb45a313c30da9f2ac3b7ba9208dcf8f.pdf |
| work_keys_str_mv | AT nargesnaseri enhancedcytotoxicityofauraptenetoprostatecancercellsbydextrancoatedfe3o4nanoparticles AT mehrdadiranshahi enhancedcytotoxicityofauraptenetoprostatecancercellsbydextrancoatedfe3o4nanoparticles AT zahratayaraninajaran enhancedcytotoxicityofauraptenetoprostatecancercellsbydextrancoatedfe3o4nanoparticles AT salehrakhshani enhancedcytotoxicityofauraptenetoprostatecancercellsbydextrancoatedfe3o4nanoparticles AT leilamohtashami enhancedcytotoxicityofauraptenetoprostatecancercellsbydextrancoatedfe3o4nanoparticles |