Characterisation of Species and Diversity of Anopheles gambiae Keele Colony.
Anopheles gambiae sensu stricto was recently reclassified as two species, An. coluzzii and An. gambiae s.s., in wild-caught mosquitoes, on the basis of the molecular form, denoted M or S, of a marker on the X chromosome. The An. gambiae Keele line is an outbred laboratory colony strain that was deve...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5199079?pdf=render |
| _version_ | 1811268925730586624 |
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| author | Lisa C Ranford-Cartwright Sion McGeechan Donald Inch Graeme Smart Lenka Richterová Jonathan M Mwangi |
| author_facet | Lisa C Ranford-Cartwright Sion McGeechan Donald Inch Graeme Smart Lenka Richterová Jonathan M Mwangi |
| author_sort | Lisa C Ranford-Cartwright |
| collection | DOAJ |
| description | Anopheles gambiae sensu stricto was recently reclassified as two species, An. coluzzii and An. gambiae s.s., in wild-caught mosquitoes, on the basis of the molecular form, denoted M or S, of a marker on the X chromosome. The An. gambiae Keele line is an outbred laboratory colony strain that was developed around 12 years ago by crosses between mosquitoes from 4 existing An. gambiae colonies. Laboratory colonies of mosquitoes often have limited genetic diversity because of small starting populations (founder effect) and subsequent fluctuations in colony size. Here we describe the characterisation of the chromosomal form(s) present in the Keele line, and investigate the diversity present in the colony using microsatellite markers on chromosome 3. We also characterise the large 2La inversion on chromosome 2. The results indicate that only the M-form of the chromosome X marker is present in the Keele colony, which was unexpected given that 3 of the 4 parent colonies were probably S-form. Levels of diversity were relatively high, as indicated by a mean number of microsatellite alleles of 6.25 across 4 microsatellites, in at least 25 mosquitoes. Both karyotypes of the inversion on chromosome 2 (2La/2L+a) were found to be present at approximately equal proportions. The Keele colony has a mixed M- and S-form origin, and in common with the PEST strain, we propose continuing to denote it as an An. gambiae s.s. line. |
| first_indexed | 2024-04-12T21:31:35Z |
| format | Article |
| id | doaj.art-cc11ee7af9b74b449b3d940792f06c43 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T21:31:35Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cc11ee7af9b74b449b3d940792f06c432022-12-22T03:16:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011112e016899910.1371/journal.pone.0168999Characterisation of Species and Diversity of Anopheles gambiae Keele Colony.Lisa C Ranford-CartwrightSion McGeechanDonald InchGraeme SmartLenka RichterováJonathan M MwangiAnopheles gambiae sensu stricto was recently reclassified as two species, An. coluzzii and An. gambiae s.s., in wild-caught mosquitoes, on the basis of the molecular form, denoted M or S, of a marker on the X chromosome. The An. gambiae Keele line is an outbred laboratory colony strain that was developed around 12 years ago by crosses between mosquitoes from 4 existing An. gambiae colonies. Laboratory colonies of mosquitoes often have limited genetic diversity because of small starting populations (founder effect) and subsequent fluctuations in colony size. Here we describe the characterisation of the chromosomal form(s) present in the Keele line, and investigate the diversity present in the colony using microsatellite markers on chromosome 3. We also characterise the large 2La inversion on chromosome 2. The results indicate that only the M-form of the chromosome X marker is present in the Keele colony, which was unexpected given that 3 of the 4 parent colonies were probably S-form. Levels of diversity were relatively high, as indicated by a mean number of microsatellite alleles of 6.25 across 4 microsatellites, in at least 25 mosquitoes. Both karyotypes of the inversion on chromosome 2 (2La/2L+a) were found to be present at approximately equal proportions. The Keele colony has a mixed M- and S-form origin, and in common with the PEST strain, we propose continuing to denote it as an An. gambiae s.s. line.http://europepmc.org/articles/PMC5199079?pdf=render |
| spellingShingle | Lisa C Ranford-Cartwright Sion McGeechan Donald Inch Graeme Smart Lenka Richterová Jonathan M Mwangi Characterisation of Species and Diversity of Anopheles gambiae Keele Colony. PLoS ONE |
| title | Characterisation of Species and Diversity of Anopheles gambiae Keele Colony. |
| title_full | Characterisation of Species and Diversity of Anopheles gambiae Keele Colony. |
| title_fullStr | Characterisation of Species and Diversity of Anopheles gambiae Keele Colony. |
| title_full_unstemmed | Characterisation of Species and Diversity of Anopheles gambiae Keele Colony. |
| title_short | Characterisation of Species and Diversity of Anopheles gambiae Keele Colony. |
| title_sort | characterisation of species and diversity of anopheles gambiae keele colony |
| url | http://europepmc.org/articles/PMC5199079?pdf=render |
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