Influence of laboratory animal hosts on the life cycle of Hyalomma marginatum and implications for an in vivo transmission model for Crimean-Congo hemorrhagic fever virus
Crimean-Congo hemorrhagic fever virus (CCHFV) is one of the most geographically widespread arboviruses and causes a severe hemorrhagic syndrome in humans. The virus circulates in nature in a vertebrate-tick cycle and ticks of the genus Hyalomma are the main vectors and reservoirs. Although the tick...
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| Format: | Article |
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Frontiers Media S.A.
2013-08-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcimb.2013.00039/full |
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| author | Aysen eGargili Aysen eGargili Aysen eGargili Saravanan eThangamani Saravanan eThangamani Dennis A Bente Dennis A Bente |
| author_facet | Aysen eGargili Aysen eGargili Aysen eGargili Saravanan eThangamani Saravanan eThangamani Dennis A Bente Dennis A Bente |
| author_sort | Aysen eGargili |
| collection | DOAJ |
| description | Crimean-Congo hemorrhagic fever virus (CCHFV) is one of the most geographically widespread arboviruses and causes a severe hemorrhagic syndrome in humans. The virus circulates in nature in a vertebrate-tick cycle and ticks of the genus Hyalomma are the main vectors and reservoirs. Although the tick vector plays a central role in the maintenance and transmission of CCHFV in nature, comparatively little is known of CCHFV-tick interactions. This is mostly due to the fact that establishing tick colonies is laborious, and working with CCHFV requires a biosafety level 4 laboratory (BSL4) in many countries. Nonetheless, an in vivo transmission model is essential to understand the epidemiology of the transmission cycle of CCHFV. In addition, important parameters such as vectorial capacity of tick species, levels of infection in the host necessary to infect the tick, and aspects of virus transmission by tick bite including the influence of tick saliva, cannot be investigated any other way. Here, we evaluate the influence of different laboratory animal species as hosts supporting the life cycle of Hyalomma marginatum, a two-host tick. Rabbits were considered the host of choice for the maintenance of the uninfected colonies due to high larval attachment rates, shorter larval-nymphal feeding times, higher nymphal molting rates, high egg hatching rates and higher conversion efficiency index. Furthermore, we describe the successful establishment of an in vivo transmission model CCHFV in a BSL4 biocontainment setting using interferon knockout mice. This will give us a new tool to study the transmission and interaction of CCHFV with its tick vector. |
| first_indexed | 2024-12-10T14:36:07Z |
| format | Article |
| id | doaj.art-cc12d5b9b05b46e4a689a0da611c940e |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-10T14:36:07Z |
| publishDate | 2013-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-cc12d5b9b05b46e4a689a0da611c940e2022-12-22T01:44:50ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882013-08-01310.3389/fcimb.2013.0003955876Influence of laboratory animal hosts on the life cycle of Hyalomma marginatum and implications for an in vivo transmission model for Crimean-Congo hemorrhagic fever virusAysen eGargili0Aysen eGargili1Aysen eGargili2Saravanan eThangamani3Saravanan eThangamani4Dennis A Bente5Dennis A Bente6The University of Texas Medical BranchGalveston National LaboratoryMarmara UniversityGalveston National LaboratoryUniversity of Texas Medical BranchGalveston National LaboratoryThe University of Texas Medical BranchCrimean-Congo hemorrhagic fever virus (CCHFV) is one of the most geographically widespread arboviruses and causes a severe hemorrhagic syndrome in humans. The virus circulates in nature in a vertebrate-tick cycle and ticks of the genus Hyalomma are the main vectors and reservoirs. Although the tick vector plays a central role in the maintenance and transmission of CCHFV in nature, comparatively little is known of CCHFV-tick interactions. This is mostly due to the fact that establishing tick colonies is laborious, and working with CCHFV requires a biosafety level 4 laboratory (BSL4) in many countries. Nonetheless, an in vivo transmission model is essential to understand the epidemiology of the transmission cycle of CCHFV. In addition, important parameters such as vectorial capacity of tick species, levels of infection in the host necessary to infect the tick, and aspects of virus transmission by tick bite including the influence of tick saliva, cannot be investigated any other way. Here, we evaluate the influence of different laboratory animal species as hosts supporting the life cycle of Hyalomma marginatum, a two-host tick. Rabbits were considered the host of choice for the maintenance of the uninfected colonies due to high larval attachment rates, shorter larval-nymphal feeding times, higher nymphal molting rates, high egg hatching rates and higher conversion efficiency index. Furthermore, we describe the successful establishment of an in vivo transmission model CCHFV in a BSL4 biocontainment setting using interferon knockout mice. This will give us a new tool to study the transmission and interaction of CCHFV with its tick vector.http://journal.frontiersin.org/Journal/10.3389/fcimb.2013.00039/fullNairovirusTick InfestationsTick-Borne DiseasesTicksBSL-4bunyavirus |
| spellingShingle | Aysen eGargili Aysen eGargili Aysen eGargili Saravanan eThangamani Saravanan eThangamani Dennis A Bente Dennis A Bente Influence of laboratory animal hosts on the life cycle of Hyalomma marginatum and implications for an in vivo transmission model for Crimean-Congo hemorrhagic fever virus Frontiers in Cellular and Infection Microbiology Nairovirus Tick Infestations Tick-Borne Diseases Ticks BSL-4 bunyavirus |
| title | Influence of laboratory animal hosts on the life cycle of Hyalomma marginatum and implications for an in vivo transmission model for Crimean-Congo hemorrhagic fever virus |
| title_full | Influence of laboratory animal hosts on the life cycle of Hyalomma marginatum and implications for an in vivo transmission model for Crimean-Congo hemorrhagic fever virus |
| title_fullStr | Influence of laboratory animal hosts on the life cycle of Hyalomma marginatum and implications for an in vivo transmission model for Crimean-Congo hemorrhagic fever virus |
| title_full_unstemmed | Influence of laboratory animal hosts on the life cycle of Hyalomma marginatum and implications for an in vivo transmission model for Crimean-Congo hemorrhagic fever virus |
| title_short | Influence of laboratory animal hosts on the life cycle of Hyalomma marginatum and implications for an in vivo transmission model for Crimean-Congo hemorrhagic fever virus |
| title_sort | influence of laboratory animal hosts on the life cycle of hyalomma marginatum and implications for an in vivo transmission model for crimean congo hemorrhagic fever virus |
| topic | Nairovirus Tick Infestations Tick-Borne Diseases Ticks BSL-4 bunyavirus |
| url | http://journal.frontiersin.org/Journal/10.3389/fcimb.2013.00039/full |
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