Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation
Injecting Cas9 and gRNA into an animal zygote often produces mosaicism and random biallelic targeting. Here, the authors use pronuclear transfer to reduce mosaicism and selectively target parental alleles.
Bibliographic Details
| Main Authors: |
Yanhe Li,
Yuteng Weng,
Dandan Bai,
Yanping Jia,
Yingdong Liu,
Yalin Zhang,
Xiaochen Kou,
Yanhong Zhao,
Jingling Ruan,
Jiayu Chen,
Jiqing Yin,
Hong Wang,
Xiaoming Teng,
Zuolin Wang,
Wenqiang Liu,
Shaorong Gao |
|---|
| Format: | Article
|
|---|
| Language: | English |
|---|
| Published: |
Nature Portfolio
2020-09-01
|
|---|
| Series: | Nature Communications
|
|---|
| Online Access: | https://doi.org/10.1038/s41467-020-18391-y
|
|---|