Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation
Injecting Cas9 and gRNA into an animal zygote often produces mosaicism and random biallelic targeting. Here, the authors use pronuclear transfer to reduce mosaicism and selectively target parental alleles.
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-09-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-18391-y |
| _version_ | 1818845936132030464 |
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| author | Yanhe Li Yuteng Weng Dandan Bai Yanping Jia Yingdong Liu Yalin Zhang Xiaochen Kou Yanhong Zhao Jingling Ruan Jiayu Chen Jiqing Yin Hong Wang Xiaoming Teng Zuolin Wang Wenqiang Liu Shaorong Gao |
| author_facet | Yanhe Li Yuteng Weng Dandan Bai Yanping Jia Yingdong Liu Yalin Zhang Xiaochen Kou Yanhong Zhao Jingling Ruan Jiayu Chen Jiqing Yin Hong Wang Xiaoming Teng Zuolin Wang Wenqiang Liu Shaorong Gao |
| author_sort | Yanhe Li |
| collection | DOAJ |
| description | Injecting Cas9 and gRNA into an animal zygote often produces mosaicism and random biallelic targeting. Here, the authors use pronuclear transfer to reduce mosaicism and selectively target parental alleles. |
| first_indexed | 2024-12-19T05:37:34Z |
| format | Article |
| id | doaj.art-cc16067a91c84bc8a8ce6c62671c27c7 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T05:37:34Z |
| publishDate | 2020-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-cc16067a91c84bc8a8ce6c62671c27c72022-12-21T20:34:05ZengNature PortfolioNature Communications2041-17232020-09-0111111210.1038/s41467-020-18391-yPrecise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantationYanhe Li0Yuteng Weng1Dandan Bai2Yanping Jia3Yingdong Liu4Yalin Zhang5Xiaochen Kou6Yanhong Zhao7Jingling Ruan8Jiayu Chen9Jiqing Yin10Hong Wang11Xiaoming Teng12Zuolin Wang13Wenqiang Liu14Shaorong Gao15Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji UniversityShanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Implantology, School and Hospital of Stomatology, Tongji UniversityClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji UniversityClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji UniversityClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji UniversityClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji UniversityClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji UniversityClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji UniversityClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji UniversityClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji UniversityClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji UniversityClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji UniversityClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji UniversityShanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Implantology, School and Hospital of Stomatology, Tongji UniversityClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji UniversityClinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji UniversityInjecting Cas9 and gRNA into an animal zygote often produces mosaicism and random biallelic targeting. Here, the authors use pronuclear transfer to reduce mosaicism and selectively target parental alleles.https://doi.org/10.1038/s41467-020-18391-y |
| spellingShingle | Yanhe Li Yuteng Weng Dandan Bai Yanping Jia Yingdong Liu Yalin Zhang Xiaochen Kou Yanhong Zhao Jingling Ruan Jiayu Chen Jiqing Yin Hong Wang Xiaoming Teng Zuolin Wang Wenqiang Liu Shaorong Gao Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation Nature Communications |
| title | Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation |
| title_full | Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation |
| title_fullStr | Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation |
| title_full_unstemmed | Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation |
| title_short | Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation |
| title_sort | precise allele specific genome editing by spatiotemporal control of crispr cas9 via pronuclear transplantation |
| url | https://doi.org/10.1038/s41467-020-18391-y |
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