Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation

Precise allele-specific genome editing by spatiotemporal control of CRISPR-Cas9 via pronuclear transplantation

Injecting Cas9 and gRNA into an animal zygote often produces mosaicism and random biallelic targeting. Here, the authors use pronuclear transfer to reduce mosaicism and selectively target parental alleles.

Bibliographic Details
Main Authors: Yanhe Li, Yuteng Weng, Dandan Bai, Yanping Jia, Yingdong Liu, Yalin Zhang, Xiaochen Kou, Yanhong Zhao, Jingling Ruan, Jiayu Chen, Jiqing Yin, Hong Wang, Xiaoming Teng, Zuolin Wang, Wenqiang Liu, Shaorong Gao
Format: Article
Language:English
Published: Nature Portfolio 2020-09-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-18391-y

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