Effects of laccase and cellulase on saccharification of barley malt
Improving saccharification of barley malt is beneficial to promoting economic benefits of beer brewers, but there are few detailed reports on the application of cellulase and laccase in barley malt. So, barley malt was pretreated by cellulase and laccase, and the malt wort and brewer's spent gr...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-09-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022020321 |
| _version_ | 1828384630728818688 |
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| author | Jianguo Wu Ziyi Li Jiapei Wang Huanwei Gan Jiandong Wang Ci Jin Guilong Yan Cannan Yu Yuzhen Zhou Wei Wang |
| author_facet | Jianguo Wu Ziyi Li Jiapei Wang Huanwei Gan Jiandong Wang Ci Jin Guilong Yan Cannan Yu Yuzhen Zhou Wei Wang |
| author_sort | Jianguo Wu |
| collection | DOAJ |
| description | Improving saccharification of barley malt is beneficial to promoting economic benefits of beer brewers, but there are few detailed reports on the application of cellulase and laccase in barley malt. So, barley malt was pretreated by cellulase and laccase, and the malt wort and brewer's spent grains were analyzed by HPLC, FTIR and SEM in this study. The concentration of malt wort was increased significantly to 8.1 (° Bx), which increased by 28.6% after barley malt was pretreated by cellulase, but laccase could not improve saccharification of barley malt. Through analysis of sugar in malt wort and cellulose and lignin components as well as physical and chemical structures of brewer's spent grains, the increase in sugar content in malt wort was mainly due to the increase in glucose because of hydrolysis of cellulose in barley malt by cellulase. Furtherly, laccase and cellulase should have a mutual inhibition when they are pretreated simultaneously. |
| first_indexed | 2024-12-10T05:06:41Z |
| format | Article |
| id | doaj.art-cc177dab5c954232924aed2692cd4e98 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-10T05:06:41Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-cc177dab5c954232924aed2692cd4e982022-12-22T02:01:14ZengElsevierHeliyon2405-84402022-09-0189e10744Effects of laccase and cellulase on saccharification of barley maltJianguo Wu0Ziyi Li1Jiapei Wang2Huanwei Gan3Jiandong Wang4Ci Jin5Guilong Yan6Cannan Yu7Yuzhen Zhou8Wei Wang9Jiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Jiangsu Key Construction Laboratory for Food Safe and Nutritional Function, School of Life Science, Huaiyin Normal University, Changjiang West Road 111, Huai'an 223300, China; Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaiyin Normal University, Changjiang West Road 111, Huai'an 223300, China; Corresponding author.Jiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Jiangsu Key Construction Laboratory for Food Safe and Nutritional Function, School of Life Science, Huaiyin Normal University, Changjiang West Road 111, Huai'an 223300, ChinaJiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Jiangsu Key Construction Laboratory for Food Safe and Nutritional Function, School of Life Science, Huaiyin Normal University, Changjiang West Road 111, Huai'an 223300, ChinaJiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Jiangsu Key Construction Laboratory for Food Safe and Nutritional Function, School of Life Science, Huaiyin Normal University, Changjiang West Road 111, Huai'an 223300, ChinaJiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Jiangsu Key Construction Laboratory for Food Safe and Nutritional Function, School of Life Science, Huaiyin Normal University, Changjiang West Road 111, Huai'an 223300, ChinaJiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Jiangsu Key Construction Laboratory for Food Safe and Nutritional Function, School of Life Science, Huaiyin Normal University, Changjiang West Road 111, Huai'an 223300, China; Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaiyin Normal University, Changjiang West Road 111, Huai'an 223300, ChinaJiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Jiangsu Key Construction Laboratory for Food Safe and Nutritional Function, School of Life Science, Huaiyin Normal University, Changjiang West Road 111, Huai'an 223300, China; Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaiyin Normal University, Changjiang West Road 111, Huai'an 223300, ChinaJiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Jiangsu Key Construction Laboratory for Food Safe and Nutritional Function, School of Life Science, Huaiyin Normal University, Changjiang West Road 111, Huai'an 223300, ChinaJiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Jiangsu Key Construction Laboratory for Food Safe and Nutritional Function, School of Life Science, Huaiyin Normal University, Changjiang West Road 111, Huai'an 223300, China; Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaiyin Normal University, Changjiang West Road 111, Huai'an 223300, ChinaJiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Jiangsu Key Construction Laboratory for Food Safe and Nutritional Function, School of Life Science, Huaiyin Normal University, Changjiang West Road 111, Huai'an 223300, China; Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaiyin Normal University, Changjiang West Road 111, Huai'an 223300, ChinaImproving saccharification of barley malt is beneficial to promoting economic benefits of beer brewers, but there are few detailed reports on the application of cellulase and laccase in barley malt. So, barley malt was pretreated by cellulase and laccase, and the malt wort and brewer's spent grains were analyzed by HPLC, FTIR and SEM in this study. The concentration of malt wort was increased significantly to 8.1 (° Bx), which increased by 28.6% after barley malt was pretreated by cellulase, but laccase could not improve saccharification of barley malt. Through analysis of sugar in malt wort and cellulose and lignin components as well as physical and chemical structures of brewer's spent grains, the increase in sugar content in malt wort was mainly due to the increase in glucose because of hydrolysis of cellulose in barley malt by cellulase. Furtherly, laccase and cellulase should have a mutual inhibition when they are pretreated simultaneously.http://www.sciencedirect.com/science/article/pii/S2405844022020321Barley maltSaccharificationBrewer's spent grainsCellulaseLaccase |
| spellingShingle | Jianguo Wu Ziyi Li Jiapei Wang Huanwei Gan Jiandong Wang Ci Jin Guilong Yan Cannan Yu Yuzhen Zhou Wei Wang Effects of laccase and cellulase on saccharification of barley malt Heliyon Barley malt Saccharification Brewer's spent grains Cellulase Laccase |
| title | Effects of laccase and cellulase on saccharification of barley malt |
| title_full | Effects of laccase and cellulase on saccharification of barley malt |
| title_fullStr | Effects of laccase and cellulase on saccharification of barley malt |
| title_full_unstemmed | Effects of laccase and cellulase on saccharification of barley malt |
| title_short | Effects of laccase and cellulase on saccharification of barley malt |
| title_sort | effects of laccase and cellulase on saccharification of barley malt |
| topic | Barley malt Saccharification Brewer's spent grains Cellulase Laccase |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022020321 |
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