An evolutionary approach to recover genes predominantly expressed in the testes of the zebrafish, chicken and mouse
Abstract Background Previously, we have demonstrated that genes involved in ovarian function are highly conserved throughout evolution. In this study, we aimed to document the conservation of genes involved in spermatogenesis from flies to vertebrates and their expression profiles in vertebrates. Re...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-07-01
|
| Series: | BMC Evolutionary Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12862-019-1462-8 |
| _version_ | 1818695732637466624 |
|---|---|
| author | Sophie Fouchécourt Floriane Picolo Sébastien Elis Charlotte Lécureuil Aurore Thélie Marina Govoroun Mégane Brégeon Pascal Papillier Jean-Jacques Lareyre Philippe Monget |
| author_facet | Sophie Fouchécourt Floriane Picolo Sébastien Elis Charlotte Lécureuil Aurore Thélie Marina Govoroun Mégane Brégeon Pascal Papillier Jean-Jacques Lareyre Philippe Monget |
| author_sort | Sophie Fouchécourt |
| collection | DOAJ |
| description | Abstract Background Previously, we have demonstrated that genes involved in ovarian function are highly conserved throughout evolution. In this study, we aimed to document the conservation of genes involved in spermatogenesis from flies to vertebrates and their expression profiles in vertebrates. Results We retrieved 379 Drosophila melanogaster genes that are functionally involved in male reproduction according to their mutant phenotypes and listed their vertebrate orthologs. 83% of the fly genes have at least one vertebrate ortholog for a total of 625 mouse orthologs. This conservation percentage is almost twice as high as the 42% rate for the whole fly genome and is similar to that previously found for genes preferentially expressed in ovaries. Of the 625 mouse orthologs, we selected 68 mouse genes of interest, 42 of which exhibited a predominant relative expression in testes and 26 were their paralogs. These 68 mouse genes exhibited 144 and 60 orthologs in chicken and zebrafish, respectively, gathered in 28 groups of paralogs. Almost two thirds of the chicken orthologs and half of the zebrafish orthologs exhibited a relative expression ≥50% in testis. Finally, our focus on functional in silico data demonstrated that most of these genes were involved in the germ cell process, primarily in structure elaboration/maintenance and in acid nucleic metabolism. Conclusion Our work confirms that the genes involved in germ cell development are highly conserved across evolution in vertebrates and invertebrates and display a high rate of conservation of preferential testicular expression among vertebrates. Among the genes highlighted in this study, three mouse genes (Lrrc46, Pabpc6 and Pkd2l1) have not previously been described in the testes, neither their zebrafish nor chicken orthologs. The phylogenetic approach developed in this study finally allows considering new testicular genes for further fundamental studies in vertebrates, including model species (mouse and zebrafish). |
| first_indexed | 2024-12-17T13:50:09Z |
| format | Article |
| id | doaj.art-cc18ac64cb2b4b8b90034a8c8035db69 |
| institution | Directory Open Access Journal |
| issn | 1471-2148 |
| language | English |
| last_indexed | 2024-12-17T13:50:09Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Evolutionary Biology |
| spelling | doaj.art-cc18ac64cb2b4b8b90034a8c8035db692022-12-21T21:46:04ZengBMCBMC Evolutionary Biology1471-21482019-07-0119111510.1186/s12862-019-1462-8An evolutionary approach to recover genes predominantly expressed in the testes of the zebrafish, chicken and mouseSophie Fouchécourt0Floriane Picolo1Sébastien Elis2Charlotte Lécureuil3Aurore Thélie4Marina Govoroun5Mégane Brégeon6Pascal Papillier7Jean-Jacques Lareyre8Philippe Monget9PRC, CNRS, IFCE, INRA, Université de ToursPRC, CNRS, IFCE, INRA, Université de ToursPRC, CNRS, IFCE, INRA, Université de ToursInstitut de Recherche sur la Biologie de l’Insecte (IRBI), UMR 7261, CNRS-Université de ToursPRC, CNRS, IFCE, INRA, Université de ToursPRC, CNRS, IFCE, INRA, Université de ToursPRC, CNRS, IFCE, INRA, Université de ToursPRC, CNRS, IFCE, INRA, Université de ToursINRA, UPR 1037, Laboratory of Fish Physiology and Genomics (LPGP), BIOSIT, OUEST-genopole, Bât. 16, Campus de BeaulieuPRC, CNRS, IFCE, INRA, Université de ToursAbstract Background Previously, we have demonstrated that genes involved in ovarian function are highly conserved throughout evolution. In this study, we aimed to document the conservation of genes involved in spermatogenesis from flies to vertebrates and their expression profiles in vertebrates. Results We retrieved 379 Drosophila melanogaster genes that are functionally involved in male reproduction according to their mutant phenotypes and listed their vertebrate orthologs. 83% of the fly genes have at least one vertebrate ortholog for a total of 625 mouse orthologs. This conservation percentage is almost twice as high as the 42% rate for the whole fly genome and is similar to that previously found for genes preferentially expressed in ovaries. Of the 625 mouse orthologs, we selected 68 mouse genes of interest, 42 of which exhibited a predominant relative expression in testes and 26 were their paralogs. These 68 mouse genes exhibited 144 and 60 orthologs in chicken and zebrafish, respectively, gathered in 28 groups of paralogs. Almost two thirds of the chicken orthologs and half of the zebrafish orthologs exhibited a relative expression ≥50% in testis. Finally, our focus on functional in silico data demonstrated that most of these genes were involved in the germ cell process, primarily in structure elaboration/maintenance and in acid nucleic metabolism. Conclusion Our work confirms that the genes involved in germ cell development are highly conserved across evolution in vertebrates and invertebrates and display a high rate of conservation of preferential testicular expression among vertebrates. Among the genes highlighted in this study, three mouse genes (Lrrc46, Pabpc6 and Pkd2l1) have not previously been described in the testes, neither their zebrafish nor chicken orthologs. The phylogenetic approach developed in this study finally allows considering new testicular genes for further fundamental studies in vertebrates, including model species (mouse and zebrafish).http://link.springer.com/article/10.1186/s12862-019-1462-8Gene evolutionConservationTestisSpermatogenesis |
| spellingShingle | Sophie Fouchécourt Floriane Picolo Sébastien Elis Charlotte Lécureuil Aurore Thélie Marina Govoroun Mégane Brégeon Pascal Papillier Jean-Jacques Lareyre Philippe Monget An evolutionary approach to recover genes predominantly expressed in the testes of the zebrafish, chicken and mouse BMC Evolutionary Biology Gene evolution Conservation Testis Spermatogenesis |
| title | An evolutionary approach to recover genes predominantly expressed in the testes of the zebrafish, chicken and mouse |
| title_full | An evolutionary approach to recover genes predominantly expressed in the testes of the zebrafish, chicken and mouse |
| title_fullStr | An evolutionary approach to recover genes predominantly expressed in the testes of the zebrafish, chicken and mouse |
| title_full_unstemmed | An evolutionary approach to recover genes predominantly expressed in the testes of the zebrafish, chicken and mouse |
| title_short | An evolutionary approach to recover genes predominantly expressed in the testes of the zebrafish, chicken and mouse |
| title_sort | evolutionary approach to recover genes predominantly expressed in the testes of the zebrafish chicken and mouse |
| topic | Gene evolution Conservation Testis Spermatogenesis |
| url | http://link.springer.com/article/10.1186/s12862-019-1462-8 |
| work_keys_str_mv | AT sophiefouchecourt anevolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT florianepicolo anevolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT sebastienelis anevolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT charlottelecureuil anevolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT aurorethelie anevolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT marinagovoroun anevolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT meganebregeon anevolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT pascalpapillier anevolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT jeanjacqueslareyre anevolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT philippemonget anevolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT sophiefouchecourt evolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT florianepicolo evolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT sebastienelis evolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT charlottelecureuil evolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT aurorethelie evolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT marinagovoroun evolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT meganebregeon evolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT pascalpapillier evolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT jeanjacqueslareyre evolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse AT philippemonget evolutionaryapproachtorecovergenespredominantlyexpressedinthetestesofthezebrafishchickenandmouse |