Dual Role of Vitamin C-Encapsulated Liposomal Berberine in Effective Colon Anticancer Immunotherapy
The aim of the study was to achieve effective colon anticancer immunotherapy using the alkaloid berberine. In the presented paper we attempt to develop a formulation of berberine loaded into liposomal carriers using the vitamin C gradient method, characterized by efficient drug encapsulation, high s...
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MDPI AG
2023-12-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/17/1/5 |
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| author | Martyna Mianowska Magdalena Zaremba-Czogalla Adrianna Zygmunt Mohamed Mahmud Regine Süss Jerzy Gubernator |
| author_facet | Martyna Mianowska Magdalena Zaremba-Czogalla Adrianna Zygmunt Mohamed Mahmud Regine Süss Jerzy Gubernator |
| author_sort | Martyna Mianowska |
| collection | DOAJ |
| description | The aim of the study was to achieve effective colon anticancer immunotherapy using the alkaloid berberine. In the presented paper we attempt to develop a formulation of berberine loaded into liposomal carriers using the vitamin C gradient method, characterized by efficient drug encapsulation, high stability during long-term storage, low drug release in human plasma with specific cytotoxicity towards colon cancer cells. Liposomal berberine was responsible for the induction of oxidative stress, the presence of Ca<sup>2+</sup> ions in the cytosol, the reduction of Δψm, and ATP depletion with a simultaneous lack of caspase activity. Moreover, treatment with liposomal berberine led to CRT exposure on the surface of cancer cells, extracellular ATP, and HMGB1 release. The above-described mechanism of action was most likely associated with ICD induction, contributing to the increased number of phagocytic cancer cells. We have shown that cancer cells treated with liposomal berberine were phagocytosed more frequently by macrophages compared to the untreated cancer cells. What is more, we have shown that macrophage pre-treatment with liposomal berberine led to a 3-fold change in the number of phagocytosed SW620 cancer cells. The obtained results provide new insights into the role of berberine in maintaining the immune response against colorectal cancer. |
| first_indexed | 2024-03-08T10:38:48Z |
| format | Article |
| id | doaj.art-cc1ccf7636a1413b9636ae0e3fd78ceb |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-08T10:38:48Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-cc1ccf7636a1413b9636ae0e3fd78ceb2024-01-26T18:04:20ZengMDPI AGPharmaceuticals1424-82472023-12-01171510.3390/ph17010005Dual Role of Vitamin C-Encapsulated Liposomal Berberine in Effective Colon Anticancer ImmunotherapyMartyna Mianowska0Magdalena Zaremba-Czogalla1Adrianna Zygmunt2Mohamed Mahmud3Regine Süss4Jerzy Gubernator5Department of Lipids and Liposomes, Faculty of Biotechnology, University of Wroclaw, 50-383 Wroclaw, PolandDepartment of Lipids and Liposomes, Faculty of Biotechnology, University of Wroclaw, 50-383 Wroclaw, PolandDepartment of Lipids and Liposomes, Faculty of Biotechnology, University of Wroclaw, 50-383 Wroclaw, PolandDepartment of Lipids and Liposomes, Faculty of Biotechnology, University of Wroclaw, 50-383 Wroclaw, PolandInstitute of Pharmaceutical Sciences, Department of Pharmaceutics, Albert Ludwig University of Freiburg, Sonnenstr. 5, 79104 Freiburg, GermanyDepartment of Lipids and Liposomes, Faculty of Biotechnology, University of Wroclaw, 50-383 Wroclaw, PolandThe aim of the study was to achieve effective colon anticancer immunotherapy using the alkaloid berberine. In the presented paper we attempt to develop a formulation of berberine loaded into liposomal carriers using the vitamin C gradient method, characterized by efficient drug encapsulation, high stability during long-term storage, low drug release in human plasma with specific cytotoxicity towards colon cancer cells. Liposomal berberine was responsible for the induction of oxidative stress, the presence of Ca<sup>2+</sup> ions in the cytosol, the reduction of Δψm, and ATP depletion with a simultaneous lack of caspase activity. Moreover, treatment with liposomal berberine led to CRT exposure on the surface of cancer cells, extracellular ATP, and HMGB1 release. The above-described mechanism of action was most likely associated with ICD induction, contributing to the increased number of phagocytic cancer cells. We have shown that cancer cells treated with liposomal berberine were phagocytosed more frequently by macrophages compared to the untreated cancer cells. What is more, we have shown that macrophage pre-treatment with liposomal berberine led to a 3-fold change in the number of phagocytosed SW620 cancer cells. The obtained results provide new insights into the role of berberine in maintaining the immune response against colorectal cancer.https://www.mdpi.com/1424-8247/17/1/5colon cancerCD47berberineliposomescalreticulinICD |
| spellingShingle | Martyna Mianowska Magdalena Zaremba-Czogalla Adrianna Zygmunt Mohamed Mahmud Regine Süss Jerzy Gubernator Dual Role of Vitamin C-Encapsulated Liposomal Berberine in Effective Colon Anticancer Immunotherapy Pharmaceuticals colon cancer CD47 berberine liposomes calreticulin ICD |
| title | Dual Role of Vitamin C-Encapsulated Liposomal Berberine in Effective Colon Anticancer Immunotherapy |
| title_full | Dual Role of Vitamin C-Encapsulated Liposomal Berberine in Effective Colon Anticancer Immunotherapy |
| title_fullStr | Dual Role of Vitamin C-Encapsulated Liposomal Berberine in Effective Colon Anticancer Immunotherapy |
| title_full_unstemmed | Dual Role of Vitamin C-Encapsulated Liposomal Berberine in Effective Colon Anticancer Immunotherapy |
| title_short | Dual Role of Vitamin C-Encapsulated Liposomal Berberine in Effective Colon Anticancer Immunotherapy |
| title_sort | dual role of vitamin c encapsulated liposomal berberine in effective colon anticancer immunotherapy |
| topic | colon cancer CD47 berberine liposomes calreticulin ICD |
| url | https://www.mdpi.com/1424-8247/17/1/5 |
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