Quantitative Determination of Fluoroquinolones in Contaminated Soils by HPLC with Solid-Phase Extraction
This work reports the development of an analytical method for the simultaneous analysis of three fluoroquinolones; ciprofloxacin (CIP), norfloxacin (NOR) and ofloxacin (OFL) in soil matrix. The proposed method was performed by using microwave-assisted extraction (MAE), solid-phase extraction (SPE) f...
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| Format: | Article |
| Language: | Arabic |
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College of Science for Women, University of Baghdad
2020-03-01
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| Series: | Baghdad Science Journal |
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| Online Access: | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3540 |
| _version_ | 1818173409406746624 |
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| author | bashar hussin qassim |
| author_facet | bashar hussin qassim |
| author_sort | bashar hussin qassim |
| collection | DOAJ |
| description | This work reports the development of an analytical method for the simultaneous analysis of three fluoroquinolones; ciprofloxacin (CIP), norfloxacin (NOR) and ofloxacin (OFL) in soil matrix. The proposed method was performed by using microwave-assisted extraction (MAE), solid-phase extraction (SPE) for samples purification, and finally the pre-concentrated samples were analyzed by HPLC detector. In this study, various organic solvents were tested to extract the test compounds, and the extraction performance was evaluated by testing various parameters including extraction solvent, solvent volume, extraction time, temperature and number of the extraction cycles. The current method showed a good linearity over the concentration ranging from 1 – 300ng g-1 with correlation coefficients (r2 > 0.998) for all test compounds. Good recoveries were also obtained for the test compounds ranged from 89 – 99 %. The relative standard deviation (RSD) from intra-day and inter-day precision was lower than 7%, and the detection (LOD) and quantification (LOQ) limits ranged from 0.9 – 2.7ng g-1.Finally, the proposed method was applied to determine the target compounds in real soil samples irrigated with wastewater. The results confirm the presence of the test compounds in soils with values ranging from 1.9 – 4.6ng g-1, as well as the suitability of the proposed method to determine the test compounds in real soil matrix as well as the ability of the test compounds to accumulate in soils irrigated with sewage. |
| first_indexed | 2024-12-11T19:28:03Z |
| format | Article |
| id | doaj.art-cc1ea5a8ca8848519f91477b49fb85e8 |
| institution | Directory Open Access Journal |
| issn | 2078-8665 2411-7986 |
| language | Arabic |
| last_indexed | 2024-12-11T19:28:03Z |
| publishDate | 2020-03-01 |
| publisher | College of Science for Women, University of Baghdad |
| record_format | Article |
| series | Baghdad Science Journal |
| spelling | doaj.art-cc1ea5a8ca8848519f91477b49fb85e82022-12-22T00:53:22ZaraCollege of Science for Women, University of BaghdadBaghdad Science Journal2078-86652411-79862020-03-0117110.21123/bsj.2020.17.1.0048Quantitative Determination of Fluoroquinolones in Contaminated Soils by HPLC with Solid-Phase Extractionbashar hussin qassimThis work reports the development of an analytical method for the simultaneous analysis of three fluoroquinolones; ciprofloxacin (CIP), norfloxacin (NOR) and ofloxacin (OFL) in soil matrix. The proposed method was performed by using microwave-assisted extraction (MAE), solid-phase extraction (SPE) for samples purification, and finally the pre-concentrated samples were analyzed by HPLC detector. In this study, various organic solvents were tested to extract the test compounds, and the extraction performance was evaluated by testing various parameters including extraction solvent, solvent volume, extraction time, temperature and number of the extraction cycles. The current method showed a good linearity over the concentration ranging from 1 – 300ng g-1 with correlation coefficients (r2 > 0.998) for all test compounds. Good recoveries were also obtained for the test compounds ranged from 89 – 99 %. The relative standard deviation (RSD) from intra-day and inter-day precision was lower than 7%, and the detection (LOD) and quantification (LOQ) limits ranged from 0.9 – 2.7ng g-1.Finally, the proposed method was applied to determine the target compounds in real soil samples irrigated with wastewater. The results confirm the presence of the test compounds in soils with values ranging from 1.9 – 4.6ng g-1, as well as the suitability of the proposed method to determine the test compounds in real soil matrix as well as the ability of the test compounds to accumulate in soils irrigated with sewage.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3540Contaminated soils, Fluoroquinolones, HPLC, Microwave-assisted extraction, Solid-phase extraction. |
| spellingShingle | bashar hussin qassim Quantitative Determination of Fluoroquinolones in Contaminated Soils by HPLC with Solid-Phase Extraction Baghdad Science Journal Contaminated soils, Fluoroquinolones, HPLC, Microwave-assisted extraction, Solid-phase extraction. |
| title | Quantitative Determination of Fluoroquinolones in Contaminated Soils by HPLC with Solid-Phase Extraction |
| title_full | Quantitative Determination of Fluoroquinolones in Contaminated Soils by HPLC with Solid-Phase Extraction |
| title_fullStr | Quantitative Determination of Fluoroquinolones in Contaminated Soils by HPLC with Solid-Phase Extraction |
| title_full_unstemmed | Quantitative Determination of Fluoroquinolones in Contaminated Soils by HPLC with Solid-Phase Extraction |
| title_short | Quantitative Determination of Fluoroquinolones in Contaminated Soils by HPLC with Solid-Phase Extraction |
| title_sort | quantitative determination of fluoroquinolones in contaminated soils by hplc with solid phase extraction |
| topic | Contaminated soils, Fluoroquinolones, HPLC, Microwave-assisted extraction, Solid-phase extraction. |
| url | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3540 |
| work_keys_str_mv | AT basharhussinqassim quantitativedeterminationoffluoroquinolonesincontaminatedsoilsbyhplcwithsolidphaseextraction |