Three-photon excited fluorescence imaging in neuroscience: From principles to applications
The development of three-photon microscopy (3PM) has greatly expanded the capability of imaging deep within biological tissues, enabling neuroscientists to visualize the structure and activity of neuronal populations with greater depth than two-photon imaging. In this review, we outline the history...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2023.1085682/full |
| _version_ | 1797902987862999040 |
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| author | Yujie Xiao Peng Deng Yaoguang Zhao Shasha Yang Bo Li |
| author_facet | Yujie Xiao Peng Deng Yaoguang Zhao Shasha Yang Bo Li |
| author_sort | Yujie Xiao |
| collection | DOAJ |
| description | The development of three-photon microscopy (3PM) has greatly expanded the capability of imaging deep within biological tissues, enabling neuroscientists to visualize the structure and activity of neuronal populations with greater depth than two-photon imaging. In this review, we outline the history and physical principles of 3PM technology. We cover the current techniques for improving the performance of 3PM. Furthermore, we summarize the imaging applications of 3PM for various brain regions and species. Finally, we discuss the future of 3PM applications for neuroscience. |
| first_indexed | 2024-04-10T09:25:52Z |
| format | Article |
| id | doaj.art-cc2086ea771f4cfb8ecd99bf66c4e59f |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-04-10T09:25:52Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-cc2086ea771f4cfb8ecd99bf66c4e59f2023-02-20T05:30:22ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2023-02-011710.3389/fnins.2023.10856821085682Three-photon excited fluorescence imaging in neuroscience: From principles to applicationsYujie XiaoPeng DengYaoguang ZhaoShasha YangBo LiThe development of three-photon microscopy (3PM) has greatly expanded the capability of imaging deep within biological tissues, enabling neuroscientists to visualize the structure and activity of neuronal populations with greater depth than two-photon imaging. In this review, we outline the history and physical principles of 3PM technology. We cover the current techniques for improving the performance of 3PM. Furthermore, we summarize the imaging applications of 3PM for various brain regions and species. Finally, we discuss the future of 3PM applications for neuroscience.https://www.frontiersin.org/articles/10.3389/fnins.2023.1085682/fullthree-photon excited fluorescence microscopydeep brain imagingstructural imagingfunctional imagingrodents |
| spellingShingle | Yujie Xiao Peng Deng Yaoguang Zhao Shasha Yang Bo Li Three-photon excited fluorescence imaging in neuroscience: From principles to applications Frontiers in Neuroscience three-photon excited fluorescence microscopy deep brain imaging structural imaging functional imaging rodents |
| title | Three-photon excited fluorescence imaging in neuroscience: From principles to applications |
| title_full | Three-photon excited fluorescence imaging in neuroscience: From principles to applications |
| title_fullStr | Three-photon excited fluorescence imaging in neuroscience: From principles to applications |
| title_full_unstemmed | Three-photon excited fluorescence imaging in neuroscience: From principles to applications |
| title_short | Three-photon excited fluorescence imaging in neuroscience: From principles to applications |
| title_sort | three photon excited fluorescence imaging in neuroscience from principles to applications |
| topic | three-photon excited fluorescence microscopy deep brain imaging structural imaging functional imaging rodents |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2023.1085682/full |
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