The Effect of Disinfectants on Quinolone Resistant <i>E. coli</i> (QREC) in Biofilm
The aim of disinfection is to reduce the number of microorganisms on surfaces which is a challenge due to biofilms. In the present study, six quinolone resistant <i>Escherichia coli</i> (QREC) strains with three different biofilm matrix compositions were included to assess the log<sub...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/11/1831 |
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| author | Ane Mohr Osland Lene K. Vestby Live L. Nesse |
| author_facet | Ane Mohr Osland Lene K. Vestby Live L. Nesse |
| author_sort | Ane Mohr Osland |
| collection | DOAJ |
| description | The aim of disinfection is to reduce the number of microorganisms on surfaces which is a challenge due to biofilms. In the present study, six quinolone resistant <i>Escherichia coli</i> (QREC) strains with three different biofilm matrix compositions were included to assess the log<sub>10</sub> colony forming units (CFU) reduction effect of three disinfectants at various exposure times on biofilm of different ages and morphotypes. Biofilm was formed on stainless steel coupons for two and five days before transferred to tubes with Virocid 0, 25%, VirkonS 1%, and TP990 1% and left for various exposure times. The biofilms were scraped off and serial dilutions were spread on blood agar plates where colony forming units (CFU) were counted. A mean log<sub>10</sub> CFU reduction ≥4 was seen on two-day-old biofilm with VirkonS and Virocid (30 min) but not on five-day old biofilm. TP990 did not display sufficient effect under the conditions tested. The bactericidal effect was inferior to that reported on planktonic bacteria. The findings of this study should be considered when establishing both disinfectant routines and standard susceptibility tests, which further should accommodate <i>E. coli</i> biofilms and not only <i>Pseudomonas</i> as is the case today. |
| first_indexed | 2024-03-10T14:42:21Z |
| format | Article |
| id | doaj.art-cc24210bbca4493e802195f17b1246a9 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T14:42:21Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-cc24210bbca4493e802195f17b1246a92023-11-20T21:40:53ZengMDPI AGMicroorganisms2076-26072020-11-01811183110.3390/microorganisms8111831The Effect of Disinfectants on Quinolone Resistant <i>E. coli</i> (QREC) in BiofilmAne Mohr Osland0Lene K. Vestby1Live L. Nesse2Norwegian Veterinary Institute, Ullevålsvn 68, P.O. Box 750 Sentrum, 0106 Oslo, NorwayNorwegian Veterinary Institute, Ullevålsvn 68, P.O. Box 750 Sentrum, 0106 Oslo, NorwayNorwegian Veterinary Institute, Ullevålsvn 68, P.O. Box 750 Sentrum, 0106 Oslo, NorwayThe aim of disinfection is to reduce the number of microorganisms on surfaces which is a challenge due to biofilms. In the present study, six quinolone resistant <i>Escherichia coli</i> (QREC) strains with three different biofilm matrix compositions were included to assess the log<sub>10</sub> colony forming units (CFU) reduction effect of three disinfectants at various exposure times on biofilm of different ages and morphotypes. Biofilm was formed on stainless steel coupons for two and five days before transferred to tubes with Virocid 0, 25%, VirkonS 1%, and TP990 1% and left for various exposure times. The biofilms were scraped off and serial dilutions were spread on blood agar plates where colony forming units (CFU) were counted. A mean log<sub>10</sub> CFU reduction ≥4 was seen on two-day-old biofilm with VirkonS and Virocid (30 min) but not on five-day old biofilm. TP990 did not display sufficient effect under the conditions tested. The bactericidal effect was inferior to that reported on planktonic bacteria. The findings of this study should be considered when establishing both disinfectant routines and standard susceptibility tests, which further should accommodate <i>E. coli</i> biofilms and not only <i>Pseudomonas</i> as is the case today.https://www.mdpi.com/2076-2607/8/11/1831biofilmQRECdisinfection<i>E. coli</i>antimicrobial resistance |
| spellingShingle | Ane Mohr Osland Lene K. Vestby Live L. Nesse The Effect of Disinfectants on Quinolone Resistant <i>E. coli</i> (QREC) in Biofilm Microorganisms biofilm QREC disinfection <i>E. coli</i> antimicrobial resistance |
| title | The Effect of Disinfectants on Quinolone Resistant <i>E. coli</i> (QREC) in Biofilm |
| title_full | The Effect of Disinfectants on Quinolone Resistant <i>E. coli</i> (QREC) in Biofilm |
| title_fullStr | The Effect of Disinfectants on Quinolone Resistant <i>E. coli</i> (QREC) in Biofilm |
| title_full_unstemmed | The Effect of Disinfectants on Quinolone Resistant <i>E. coli</i> (QREC) in Biofilm |
| title_short | The Effect of Disinfectants on Quinolone Resistant <i>E. coli</i> (QREC) in Biofilm |
| title_sort | effect of disinfectants on quinolone resistant i e coli i qrec in biofilm |
| topic | biofilm QREC disinfection <i>E. coli</i> antimicrobial resistance |
| url | https://www.mdpi.com/2076-2607/8/11/1831 |
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