Genetics of cryptic speciation within an Arctic mustard, Draba nivalis.
Crossing experiments indicate that hybrid sterility barriers frequently have developed within diploid, circumpolar plant species of the genus Draba. To gain insight into the rapid evolution of postzygotic reproductive isolation in this system, we augmented the linkage map of one of these species, D....
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3972243?pdf=render |
| _version_ | 1819090399590875136 |
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| author | A Lovisa S Gustafsson Inger Skrede Heather C Rowe Galina Gussarova Liv Borgen Loren H Rieseberg Christian Brochmann Christian Parisod |
| author_facet | A Lovisa S Gustafsson Inger Skrede Heather C Rowe Galina Gussarova Liv Borgen Loren H Rieseberg Christian Brochmann Christian Parisod |
| author_sort | A Lovisa S Gustafsson |
| collection | DOAJ |
| description | Crossing experiments indicate that hybrid sterility barriers frequently have developed within diploid, circumpolar plant species of the genus Draba. To gain insight into the rapid evolution of postzygotic reproductive isolation in this system, we augmented the linkage map of one of these species, D. nivalis, and searched for quantitative trait loci (QTLs) associated with reproductive isolation. The map adds 63 new dominant markers to a previously published dataset of 31 co-dominant microsatellites. These markers include 52 amplified fragment length polymorphisms (AFLPs) and 11 sequence-specific amplified polymorphisms (SSAPs) based on retrotransposon sequence. 22 markers displaying transmission ratio distortion were further included in the map. We resolved eight linkage groups with a total map length of 894 cM. Significant genotype-trait associations, or quantitative trait loci (QTL), were detected for reproductive phenotypes including pollen fertility (4 QTLs), seed set (3 QTLs), flowering time (3 QTLs) and number of flowers (4 QTLs). Observed patterns of inheritance were consistent with the influence of both nuclear-nuclear interactions and chromosomal changes on these traits. All seed set QTLs and one pollen fertility QTL displayed underdominant effects suggestive of the involvement of chromosomal rearrangements in hybrid sterility. Interestingly, D. nivalis is predominantly self-fertilizing, which may facilitate the establishment of underdominant loci and contribute to reproductive isolation. |
| first_indexed | 2024-12-21T22:23:13Z |
| format | Article |
| id | doaj.art-cc2462f2630c4268ac7d76fab7d5d73d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T22:23:13Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cc2462f2630c4268ac7d76fab7d5d73d2022-12-21T18:48:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9383410.1371/journal.pone.0093834Genetics of cryptic speciation within an Arctic mustard, Draba nivalis.A Lovisa S GustafssonInger SkredeHeather C RoweGalina GussarovaLiv BorgenLoren H RiesebergChristian BrochmannChristian ParisodCrossing experiments indicate that hybrid sterility barriers frequently have developed within diploid, circumpolar plant species of the genus Draba. To gain insight into the rapid evolution of postzygotic reproductive isolation in this system, we augmented the linkage map of one of these species, D. nivalis, and searched for quantitative trait loci (QTLs) associated with reproductive isolation. The map adds 63 new dominant markers to a previously published dataset of 31 co-dominant microsatellites. These markers include 52 amplified fragment length polymorphisms (AFLPs) and 11 sequence-specific amplified polymorphisms (SSAPs) based on retrotransposon sequence. 22 markers displaying transmission ratio distortion were further included in the map. We resolved eight linkage groups with a total map length of 894 cM. Significant genotype-trait associations, or quantitative trait loci (QTL), were detected for reproductive phenotypes including pollen fertility (4 QTLs), seed set (3 QTLs), flowering time (3 QTLs) and number of flowers (4 QTLs). Observed patterns of inheritance were consistent with the influence of both nuclear-nuclear interactions and chromosomal changes on these traits. All seed set QTLs and one pollen fertility QTL displayed underdominant effects suggestive of the involvement of chromosomal rearrangements in hybrid sterility. Interestingly, D. nivalis is predominantly self-fertilizing, which may facilitate the establishment of underdominant loci and contribute to reproductive isolation.http://europepmc.org/articles/PMC3972243?pdf=render |
| spellingShingle | A Lovisa S Gustafsson Inger Skrede Heather C Rowe Galina Gussarova Liv Borgen Loren H Rieseberg Christian Brochmann Christian Parisod Genetics of cryptic speciation within an Arctic mustard, Draba nivalis. PLoS ONE |
| title | Genetics of cryptic speciation within an Arctic mustard, Draba nivalis. |
| title_full | Genetics of cryptic speciation within an Arctic mustard, Draba nivalis. |
| title_fullStr | Genetics of cryptic speciation within an Arctic mustard, Draba nivalis. |
| title_full_unstemmed | Genetics of cryptic speciation within an Arctic mustard, Draba nivalis. |
| title_short | Genetics of cryptic speciation within an Arctic mustard, Draba nivalis. |
| title_sort | genetics of cryptic speciation within an arctic mustard draba nivalis |
| url | http://europepmc.org/articles/PMC3972243?pdf=render |
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