Role of <i>Rahnella aquatilis</i> AZO16M2 in Phosphate Solubilization and Ex Vitro Acclimatization of <i>Musa acuminata</i> var. Valery
<i>Rahnella aquatilis</i> AZO16M2, was characterized for its phosphate solubilization capacity to improve the establishment and survival of <i>Musa acuminata</i> var. Valery seedlings under ex-acclimation. Three phosphorus sources (Rock Phosphate (RF), Ca<sub>3</sub&...
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MDPI AG
2023-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/6/1596 |
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| author | Daniela Landa-Acuña Marcia Toro Ricardo Santos-Mendoza Doris Zúñiga-Dávila |
| author_facet | Daniela Landa-Acuña Marcia Toro Ricardo Santos-Mendoza Doris Zúñiga-Dávila |
| author_sort | Daniela Landa-Acuña |
| collection | DOAJ |
| description | <i>Rahnella aquatilis</i> AZO16M2, was characterized for its phosphate solubilization capacity to improve the establishment and survival of <i>Musa acuminata</i> var. Valery seedlings under ex-acclimation. Three phosphorus sources (Rock Phosphate (RF), Ca<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub> and K<sub>2</sub>HPO<sub>4</sub>) and two types of substrate (sand:vermiculite (1:1) and Premix N°8) were selected. The factorial analysis of variance (<i>p</i> < 0.05) showed that <i>R. aquatilis</i> AZO16M2 (OQ256130) solubilizes Ca<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub> in solid medium, with a Solubilization Index (SI) of 3.77 at 28 °C (pH 6.8). In liquid medium, it was observed that <i>R. aquatilis</i> produced 29.6 mg/L soluble P (pH 4.4), and synthesized organic acids (oxalic, D-gluconic, 2-ketogluconic and malic), Indole Acetic Acid (IAA) (33.90 ppm) and siderophores (+). Additionally, acid and alkaline phosphatases (2.59 and 2.56 µg pNP/mL/min) were detected. The presence of the pyrroloquinoline-quinone (PQQ) cofactor gene was confirmed. After inoculating AZO16M2 to <i>M. acuminata</i> in sand:vermiculite with RF, the chlorophyll content was 42.38 SPAD (Soil Plant Analysis Development). Aerial fresh weight (AFW), aerial dry weight (ADW) and root dry weight (RDW) were superior to the control by 64.15%, 60.53% and 43.48%, respectively. In Premix N°8 with RF and <i>R. aquatilis</i>, 8.91% longer roots were obtained, with 35.58% and 18.76% more AFW and RFW compared with the control as well as 94.45 SPAD. With Ca<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub>, values exceeded the control by 14.15% RFW, with 45.45 SPAD. <i>Rahnella aquatilis</i> AZO16M2 favored the ex-climatization of <i>M. acuminata</i> through improving seedling establishment and survival. |
| first_indexed | 2024-03-11T02:08:39Z |
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| spelling | doaj.art-cc263a50992644578e1a4f22675d37f02023-11-18T11:44:04ZengMDPI AGMicroorganisms2076-26072023-06-01116159610.3390/microorganisms11061596Role of <i>Rahnella aquatilis</i> AZO16M2 in Phosphate Solubilization and Ex Vitro Acclimatization of <i>Musa acuminata</i> var. ValeryDaniela Landa-Acuña0Marcia Toro1Ricardo Santos-Mendoza2Doris Zúñiga-Dávila3Laboratorio de Ecología Microbiana y Biotecnología, Departamento de Biología, Facultad de Ciencias, Universidad Nacional Agraria La Molina, Lima 15024, PeruLaboratorio de Ecología Microbiana y Biotecnología, Departamento de Biología, Facultad de Ciencias, Universidad Nacional Agraria La Molina, Lima 15024, PeruLaboratorio de Ecología Microbiana y Biotecnología, Departamento de Biología, Facultad de Ciencias, Universidad Nacional Agraria La Molina, Lima 15024, PeruLaboratorio de Ecología Microbiana y Biotecnología, Departamento de Biología, Facultad de Ciencias, Universidad Nacional Agraria La Molina, Lima 15024, Peru<i>Rahnella aquatilis</i> AZO16M2, was characterized for its phosphate solubilization capacity to improve the establishment and survival of <i>Musa acuminata</i> var. Valery seedlings under ex-acclimation. Three phosphorus sources (Rock Phosphate (RF), Ca<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub> and K<sub>2</sub>HPO<sub>4</sub>) and two types of substrate (sand:vermiculite (1:1) and Premix N°8) were selected. The factorial analysis of variance (<i>p</i> < 0.05) showed that <i>R. aquatilis</i> AZO16M2 (OQ256130) solubilizes Ca<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub> in solid medium, with a Solubilization Index (SI) of 3.77 at 28 °C (pH 6.8). In liquid medium, it was observed that <i>R. aquatilis</i> produced 29.6 mg/L soluble P (pH 4.4), and synthesized organic acids (oxalic, D-gluconic, 2-ketogluconic and malic), Indole Acetic Acid (IAA) (33.90 ppm) and siderophores (+). Additionally, acid and alkaline phosphatases (2.59 and 2.56 µg pNP/mL/min) were detected. The presence of the pyrroloquinoline-quinone (PQQ) cofactor gene was confirmed. After inoculating AZO16M2 to <i>M. acuminata</i> in sand:vermiculite with RF, the chlorophyll content was 42.38 SPAD (Soil Plant Analysis Development). Aerial fresh weight (AFW), aerial dry weight (ADW) and root dry weight (RDW) were superior to the control by 64.15%, 60.53% and 43.48%, respectively. In Premix N°8 with RF and <i>R. aquatilis</i>, 8.91% longer roots were obtained, with 35.58% and 18.76% more AFW and RFW compared with the control as well as 94.45 SPAD. With Ca<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub>, values exceeded the control by 14.15% RFW, with 45.45 SPAD. <i>Rahnella aquatilis</i> AZO16M2 favored the ex-climatization of <i>M. acuminata</i> through improving seedling establishment and survival.https://www.mdpi.com/2076-2607/11/6/1596pyrroloquinoline-quinone (PQQ) gene16S genetricalcium phosphateorganic acidsrock phosphateplant-growth-promoting rhizobacteria (PGPR) |
| spellingShingle | Daniela Landa-Acuña Marcia Toro Ricardo Santos-Mendoza Doris Zúñiga-Dávila Role of <i>Rahnella aquatilis</i> AZO16M2 in Phosphate Solubilization and Ex Vitro Acclimatization of <i>Musa acuminata</i> var. Valery Microorganisms pyrroloquinoline-quinone (PQQ) gene 16S gene tricalcium phosphate organic acids rock phosphate plant-growth-promoting rhizobacteria (PGPR) |
| title | Role of <i>Rahnella aquatilis</i> AZO16M2 in Phosphate Solubilization and Ex Vitro Acclimatization of <i>Musa acuminata</i> var. Valery |
| title_full | Role of <i>Rahnella aquatilis</i> AZO16M2 in Phosphate Solubilization and Ex Vitro Acclimatization of <i>Musa acuminata</i> var. Valery |
| title_fullStr | Role of <i>Rahnella aquatilis</i> AZO16M2 in Phosphate Solubilization and Ex Vitro Acclimatization of <i>Musa acuminata</i> var. Valery |
| title_full_unstemmed | Role of <i>Rahnella aquatilis</i> AZO16M2 in Phosphate Solubilization and Ex Vitro Acclimatization of <i>Musa acuminata</i> var. Valery |
| title_short | Role of <i>Rahnella aquatilis</i> AZO16M2 in Phosphate Solubilization and Ex Vitro Acclimatization of <i>Musa acuminata</i> var. Valery |
| title_sort | role of i rahnella aquatilis i azo16m2 in phosphate solubilization and ex vitro acclimatization of i musa acuminata i var valery |
| topic | pyrroloquinoline-quinone (PQQ) gene 16S gene tricalcium phosphate organic acids rock phosphate plant-growth-promoting rhizobacteria (PGPR) |
| url | https://www.mdpi.com/2076-2607/11/6/1596 |
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