Point-of-care detection of Escherichia coli O157:H7 in water using AuNPs-based aptasensor
<em><strong>Objective(s):</strong></em> Access to safe drinking and irrigation water has always been one of the major human concerns worldwide. Thus, rapid, sensitive, and inexpensive approaches for pathogenic bacteria detection, such as Escherichia coli O157:H7 (EHEC) that c...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2020-07-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_15597_df2d43f5c762737d776270cbfc2d5780.pdf |
| _version_ | 1818206656780042240 |
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| author | Vahid Soheili Seyed Mohammad Taghdisi Khalil Abnous Mohsen Ebrahimi |
| author_facet | Vahid Soheili Seyed Mohammad Taghdisi Khalil Abnous Mohsen Ebrahimi |
| author_sort | Vahid Soheili |
| collection | DOAJ |
| description | <em><strong>Objective(s):</strong></em> Access to safe drinking and irrigation water has always been one of the major human concerns worldwide. Thus, rapid, sensitive, and inexpensive approaches for pathogenic bacteria detection, such as Escherichia coli O157:H7 (EHEC) that can induce important infectious diseases, are highly on demand. <br /><em><strong>Materials and Methods:</strong></em> In this study, a sensitive aptamer-based AuNPs bioassay was developed that demonstrated its potential to detect EHEC. In the presence of the target bacterium, the specific adsorbed aptamer, leaves AuNPs surface and interacts with EHEC. The bare nanoparticles aggregate in the presence of NaCl and the color shifts from red to purple and blue depending on the bacterial concentration. <br /><em><strong>Results:</strong></em> The proposed aptasensor exhibited a good linear response over a wide concentration range of 876 to 107 CFU/ml and was closely correlated with the line equation of “y=0.0094x+0.0006” (R2= 0.9861). It also showed a low detection limit (LOD) of 263 CFU/ml (Signal/Noise=3). No response was recorded in the presence of other tested bacterial strains including Listeria monocytogenes and Salmonella typhi, indicating the high selectivity of the aptasensor. <br /><em><strong>Conclusion:</strong></em> This biosensor may be used as a smart device to screen water reservoirs and prevents the outbreak of EHEC-related life-threatening contagious diseases. |
| first_indexed | 2024-12-12T04:16:30Z |
| format | Article |
| id | doaj.art-cc29b6f935784c3e8466582eaf45ce06 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-12T04:16:30Z |
| publishDate | 2020-07-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-cc29b6f935784c3e8466582eaf45ce062022-12-22T00:38:26ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742020-07-0123790190810.22038/ijbms.2020.44016.1032215597Point-of-care detection of Escherichia coli O157:H7 in water using AuNPs-based aptasensorVahid Soheili0Seyed Mohammad Taghdisi1Khalil Abnous2Mohsen Ebrahimi3Department of Pharmacology and Toxicology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, IranTargeted Drug Delivery Research Center, Pharmaceutical Technology Institute, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranPharmaceutical Research Center, Pharmaceutical Technology Institute, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pharmacology and Toxicology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran<em><strong>Objective(s):</strong></em> Access to safe drinking and irrigation water has always been one of the major human concerns worldwide. Thus, rapid, sensitive, and inexpensive approaches for pathogenic bacteria detection, such as Escherichia coli O157:H7 (EHEC) that can induce important infectious diseases, are highly on demand. <br /><em><strong>Materials and Methods:</strong></em> In this study, a sensitive aptamer-based AuNPs bioassay was developed that demonstrated its potential to detect EHEC. In the presence of the target bacterium, the specific adsorbed aptamer, leaves AuNPs surface and interacts with EHEC. The bare nanoparticles aggregate in the presence of NaCl and the color shifts from red to purple and blue depending on the bacterial concentration. <br /><em><strong>Results:</strong></em> The proposed aptasensor exhibited a good linear response over a wide concentration range of 876 to 107 CFU/ml and was closely correlated with the line equation of “y=0.0094x+0.0006” (R2= 0.9861). It also showed a low detection limit (LOD) of 263 CFU/ml (Signal/Noise=3). No response was recorded in the presence of other tested bacterial strains including Listeria monocytogenes and Salmonella typhi, indicating the high selectivity of the aptasensor. <br /><em><strong>Conclusion:</strong></em> This biosensor may be used as a smart device to screen water reservoirs and prevents the outbreak of EHEC-related life-threatening contagious diseases.http://ijbms.mums.ac.ir/article_15597_df2d43f5c762737d776270cbfc2d5780.pdfaptameraptasensoraunpsescherichia coli o157:h7water |
| spellingShingle | Vahid Soheili Seyed Mohammad Taghdisi Khalil Abnous Mohsen Ebrahimi Point-of-care detection of Escherichia coli O157:H7 in water using AuNPs-based aptasensor Iranian Journal of Basic Medical Sciences aptamer aptasensor aunps escherichia coli o157:h7 water |
| title | Point-of-care detection of Escherichia coli O157:H7 in water using AuNPs-based aptasensor |
| title_full | Point-of-care detection of Escherichia coli O157:H7 in water using AuNPs-based aptasensor |
| title_fullStr | Point-of-care detection of Escherichia coli O157:H7 in water using AuNPs-based aptasensor |
| title_full_unstemmed | Point-of-care detection of Escherichia coli O157:H7 in water using AuNPs-based aptasensor |
| title_short | Point-of-care detection of Escherichia coli O157:H7 in water using AuNPs-based aptasensor |
| title_sort | point of care detection of escherichia coli o157 h7 in water using aunps based aptasensor |
| topic | aptamer aptasensor aunps escherichia coli o157:h7 water |
| url | http://ijbms.mums.ac.ir/article_15597_df2d43f5c762737d776270cbfc2d5780.pdf |
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