<it>luxRI </it>homologs are universally present in the genus <it>Aeromonas</it>
<p>Abstract</p> <p>Background</p> <p><it>Aeromonas </it>spp. have been regarded as "emerging pathogens". Aeromonads possess multifactorial virulence and the production of many of these virulence determinants is associated with high cell density, a...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2007-10-01
|
| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/7/93 |
| _version_ | 1818787313766891520 |
|---|---|
| author | Patole Milind S Kong Richard Jangid Kamlesh Shouche Yogesh S |
| author_facet | Patole Milind S Kong Richard Jangid Kamlesh Shouche Yogesh S |
| author_sort | Patole Milind S |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Aeromonas </it>spp. have been regarded as "emerging pathogens". Aeromonads possess multifactorial virulence and the production of many of these virulence determinants is associated with high cell density, a phenomenon that might be regulated by quorum sensing. However, only two species of the genus are reported to possess the <it>luxRI </it>quorum sensing gene homologs. The purpose of this study was to investigate if the <it>luxRI </it>homologs are universally present in the <it>Aeromonas </it>strains collected from various culture collections, clinical laboratories and field studies.</p> <p>Results</p> <p>Of all the 73 <it>Aeromonas </it>strains used in the study, seventy-one strains elicited acyl-homoserine lactone-mediated response in multiple biosensor strains. However, dot blot hybridization revealed that the <it>luxRI </it>homologs are present in all the strains. PCR amplification and sequencing revealed that the <it>luxRI </it>homologs shared a very high percentage sequence similarity. No evidence for lateral gene transfer of the <it>luxRI </it>homologs between aeromonads and other genera was noted.</p> <p>Conclusion</p> <p>We propose that the <it>luxRI </it>quorum sensing gene homologs are universally present in the genus <it>Aeromonas </it>independently from their origin. This study is the first genus-wide report of the taxonomic distribution of the <it>luxRI </it>homologs.</p> |
| first_indexed | 2024-12-18T14:05:48Z |
| format | Article |
| id | doaj.art-cc2a46031b15426b87761f50b9bbaef2 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-18T14:05:48Z |
| publishDate | 2007-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-cc2a46031b15426b87761f50b9bbaef22022-12-21T21:05:15ZengBMCBMC Microbiology1471-21802007-10-01719310.1186/1471-2180-7-93<it>luxRI </it>homologs are universally present in the genus <it>Aeromonas</it>Patole Milind SKong RichardJangid KamleshShouche Yogesh S<p>Abstract</p> <p>Background</p> <p><it>Aeromonas </it>spp. have been regarded as "emerging pathogens". Aeromonads possess multifactorial virulence and the production of many of these virulence determinants is associated with high cell density, a phenomenon that might be regulated by quorum sensing. However, only two species of the genus are reported to possess the <it>luxRI </it>quorum sensing gene homologs. The purpose of this study was to investigate if the <it>luxRI </it>homologs are universally present in the <it>Aeromonas </it>strains collected from various culture collections, clinical laboratories and field studies.</p> <p>Results</p> <p>Of all the 73 <it>Aeromonas </it>strains used in the study, seventy-one strains elicited acyl-homoserine lactone-mediated response in multiple biosensor strains. However, dot blot hybridization revealed that the <it>luxRI </it>homologs are present in all the strains. PCR amplification and sequencing revealed that the <it>luxRI </it>homologs shared a very high percentage sequence similarity. No evidence for lateral gene transfer of the <it>luxRI </it>homologs between aeromonads and other genera was noted.</p> <p>Conclusion</p> <p>We propose that the <it>luxRI </it>quorum sensing gene homologs are universally present in the genus <it>Aeromonas </it>independently from their origin. This study is the first genus-wide report of the taxonomic distribution of the <it>luxRI </it>homologs.</p>http://www.biomedcentral.com/1471-2180/7/93 |
| spellingShingle | Patole Milind S Kong Richard Jangid Kamlesh Shouche Yogesh S <it>luxRI </it>homologs are universally present in the genus <it>Aeromonas</it> BMC Microbiology |
| title | <it>luxRI </it>homologs are universally present in the genus <it>Aeromonas</it> |
| title_full | <it>luxRI </it>homologs are universally present in the genus <it>Aeromonas</it> |
| title_fullStr | <it>luxRI </it>homologs are universally present in the genus <it>Aeromonas</it> |
| title_full_unstemmed | <it>luxRI </it>homologs are universally present in the genus <it>Aeromonas</it> |
| title_short | <it>luxRI </it>homologs are universally present in the genus <it>Aeromonas</it> |
| title_sort | it luxri it homologs are universally present in the genus it aeromonas it |
| url | http://www.biomedcentral.com/1471-2180/7/93 |
| work_keys_str_mv | AT patolemilinds itluxriithomologsareuniversallypresentinthegenusitaeromonasit AT kongrichard itluxriithomologsareuniversallypresentinthegenusitaeromonasit AT jangidkamlesh itluxriithomologsareuniversallypresentinthegenusitaeromonasit AT shoucheyogeshs itluxriithomologsareuniversallypresentinthegenusitaeromonasit |