Comparison of splenocyte microRNA expression profiles of pigs during acute and chronic toxoplasmosis
Abstract Background Toxoplasma gondii is an obligate intracellular parasite that infects humans and other warm-blooded animals. Previous quantitative proteomic analyses of infected host cells revealed that the expression of many host proteins is modulated by T. gondii infection. However, at present...
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-019-5458-y |
| _version_ | 1811320472513544192 |
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| author | Zhaofeng Hou Dandan Liu Shijie Su Lele Wang Zhenxing Zhao Yifei Ma Qiaoqiao Li Chuanli Jia Jinjun Xu Yonghua Zhou Jianping Tao |
| author_facet | Zhaofeng Hou Dandan Liu Shijie Su Lele Wang Zhenxing Zhao Yifei Ma Qiaoqiao Li Chuanli Jia Jinjun Xu Yonghua Zhou Jianping Tao |
| author_sort | Zhaofeng Hou |
| collection | DOAJ |
| description | Abstract Background Toxoplasma gondii is an obligate intracellular parasite that infects humans and other warm-blooded animals. Previous quantitative proteomic analyses of infected host cells revealed that the expression of many host proteins is modulated by T. gondii infection. However, at present limited data are available on the differentially expressed miRNAs (DEMs) associated with the pathology and host immune responses induced by acute and chronic infection with T. gondii in pigs in vivo. In this study, high-throughput sequencing was used to investigate expression profiles of spleen miRNAs at 10, 25 and 50 days post-infection (DPI) in pigs infected with Chinese I genotype strain T. gondii isolated from a dead pig. Results When compared to the control group, 34, 6 and 86 DEMs were found in spleens of infected pigs at 10, 25 and 50 DPI, respectively. Gene Ontology (GO) enrichment analysis of the target genes of DEMs showed that no GO terms were enriched at 25 DPI, whereas 28 and 241 GO terms, of which two and 215 were sample-specific, were significantly enriched at 10 and 50 DPI, respectively. The top 20 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of the target genes of DEMs included signal transduction, immune system, metabolism and diseases. miRNA–gene network analysis revealed that the DEMs played important roles in the host immune response to T. gondii infection by modulating expression levels of cellular immunity-related cytokines and immune-related C-type lectins. Conclusion Our results not only showed that host miRNA expression is altered by T. gondii but also revealed differences in the regulation of key biological processes and pathways involved in host responses to acute versus chronic T. gondii infection. This will aid future research into miRNA-target interactions during T. gondii infection in pigs and the development of novel therapies against T. gondii. |
| first_indexed | 2024-04-13T12:59:57Z |
| format | Article |
| id | doaj.art-cc2aa36945a5424684b8c53cb4a5cc04 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-13T12:59:57Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-cc2aa36945a5424684b8c53cb4a5cc042022-12-22T02:45:57ZengBMCBMC Genomics1471-21642019-01-0120111510.1186/s12864-019-5458-yComparison of splenocyte microRNA expression profiles of pigs during acute and chronic toxoplasmosisZhaofeng Hou0Dandan Liu1Shijie Su2Lele Wang3Zhenxing Zhao4Yifei Ma5Qiaoqiao Li6Chuanli Jia7Jinjun Xu8Yonghua Zhou9Jianping Tao10College of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityJiangsu Institute of Parasitic DiseasesCollege of Veterinary Medicine, Yangzhou UniversityAbstract Background Toxoplasma gondii is an obligate intracellular parasite that infects humans and other warm-blooded animals. Previous quantitative proteomic analyses of infected host cells revealed that the expression of many host proteins is modulated by T. gondii infection. However, at present limited data are available on the differentially expressed miRNAs (DEMs) associated with the pathology and host immune responses induced by acute and chronic infection with T. gondii in pigs in vivo. In this study, high-throughput sequencing was used to investigate expression profiles of spleen miRNAs at 10, 25 and 50 days post-infection (DPI) in pigs infected with Chinese I genotype strain T. gondii isolated from a dead pig. Results When compared to the control group, 34, 6 and 86 DEMs were found in spleens of infected pigs at 10, 25 and 50 DPI, respectively. Gene Ontology (GO) enrichment analysis of the target genes of DEMs showed that no GO terms were enriched at 25 DPI, whereas 28 and 241 GO terms, of which two and 215 were sample-specific, were significantly enriched at 10 and 50 DPI, respectively. The top 20 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of the target genes of DEMs included signal transduction, immune system, metabolism and diseases. miRNA–gene network analysis revealed that the DEMs played important roles in the host immune response to T. gondii infection by modulating expression levels of cellular immunity-related cytokines and immune-related C-type lectins. Conclusion Our results not only showed that host miRNA expression is altered by T. gondii but also revealed differences in the regulation of key biological processes and pathways involved in host responses to acute versus chronic T. gondii infection. This will aid future research into miRNA-target interactions during T. gondii infection in pigs and the development of novel therapies against T. gondii.http://link.springer.com/article/10.1186/s12864-019-5458-yHost regulationmicroRNAPigSpleenToxoplasma gondiiAcute and chronic infection |
| spellingShingle | Zhaofeng Hou Dandan Liu Shijie Su Lele Wang Zhenxing Zhao Yifei Ma Qiaoqiao Li Chuanli Jia Jinjun Xu Yonghua Zhou Jianping Tao Comparison of splenocyte microRNA expression profiles of pigs during acute and chronic toxoplasmosis BMC Genomics Host regulation microRNA Pig Spleen Toxoplasma gondii Acute and chronic infection |
| title | Comparison of splenocyte microRNA expression profiles of pigs during acute and chronic toxoplasmosis |
| title_full | Comparison of splenocyte microRNA expression profiles of pigs during acute and chronic toxoplasmosis |
| title_fullStr | Comparison of splenocyte microRNA expression profiles of pigs during acute and chronic toxoplasmosis |
| title_full_unstemmed | Comparison of splenocyte microRNA expression profiles of pigs during acute and chronic toxoplasmosis |
| title_short | Comparison of splenocyte microRNA expression profiles of pigs during acute and chronic toxoplasmosis |
| title_sort | comparison of splenocyte microrna expression profiles of pigs during acute and chronic toxoplasmosis |
| topic | Host regulation microRNA Pig Spleen Toxoplasma gondii Acute and chronic infection |
| url | http://link.springer.com/article/10.1186/s12864-019-5458-y |
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