Mesenchymal stem cells of Oravka chicken breed: promising path to biodiversity conservation
ABSTRACT: Mesenchymal stem cells (MSCs) are multilineage cells able to differentiate into other cell types. MSCs derived from bone marrow or compact bones are the most accessible stem cells used in tissue engineering. Therefore, the aim of this study was to isolate, characterize and cryopreserve MSC...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123003267 |
| _version_ | 1797770154191355904 |
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| author | Andrea Svoradová Jaromír Vašíček Vladimír Zmrhal Eva Venusová Aleš Pavlík Miroslav Bauer Lucia Olexiková Vladimír Langraf Petr Sláma Peter Chrenek |
| author_facet | Andrea Svoradová Jaromír Vašíček Vladimír Zmrhal Eva Venusová Aleš Pavlík Miroslav Bauer Lucia Olexiková Vladimír Langraf Petr Sláma Peter Chrenek |
| author_sort | Andrea Svoradová |
| collection | DOAJ |
| description | ABSTRACT: Mesenchymal stem cells (MSCs) are multilineage cells able to differentiate into other cell types. MSCs derived from bone marrow or compact bones are the most accessible stem cells used in tissue engineering. Therefore, the aim of this study was to isolate, characterize and cryopreserve MSCs of endangered Oravka chicken breed. MSCs were obtained from compact bones of the femur and tibiotarsus. MSCs were spindle-shaped and were able to differentiate into osteo-, adipo-, and chondrocytes under the specific differentiation conditions. Furthermore, MSCs were positive for surface markers such as CD29, CD44, CD73, CD90, CD105, CD146 and negative for CD34CD45 by flow cytometry. Moreover, MSCs demonstrated high positivity of “stemness” markers aldehyde dehydrogenase, alkaline phosphatase as well as for intracellular markers vimentin, desmin, α-SMA. Subsequently, MSCs were cryopreserved using 10% dimethyl sulfoxide in liquid nitrogen. Based on the results from the viability, phenotype, and ultrastructure assessment we can concluded that the MSCs were not negatively affected by the cryopreservation. Finally, MSCs of endangered Oravka chicken breed were successfully stored in animal gene bank, thus making them a valuable genetic resource. |
| first_indexed | 2024-03-12T21:18:14Z |
| format | Article |
| id | doaj.art-cc2b2820c3c14eb6b08034a4e7ce5fc2 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-12T21:18:14Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-cc2b2820c3c14eb6b08034a4e7ce5fc22023-07-29T04:34:23ZengElsevierPoultry Science0032-57912023-08-011028102807Mesenchymal stem cells of Oravka chicken breed: promising path to biodiversity conservationAndrea Svoradová0Jaromír Vašíček1Vladimír Zmrhal2Eva Venusová3Aleš Pavlík4Miroslav Bauer5Lucia Olexiková6Vladimír Langraf7Petr Sláma8Peter Chrenek9Institute of Farm Animal Genetics and Reproduction, NPPC, Research Institute for Animal Production in Nitra, Nitra, Slovakia; Laboratory of Animal Immunology and Biotechnology, Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech RepublicInstitute of Farm Animal Genetics and Reproduction, NPPC, Research Institute for Animal Production in Nitra, Nitra, Slovakia; Institute of Biotechnology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Nitra, SlovakiaLaboratory of Animal Immunology and Biotechnology, Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech RepublicLaboratory of Animal Immunology and Biotechnology, Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech RepublicLaboratory of Animal Physiology, Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech RepublicInstitute of Farm Animal Genetics and Reproduction, NPPC, Research Institute for Animal Production in Nitra, Nitra, Slovakia; Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, SlovakiaInstitute of Farm Animal Genetics and Reproduction, NPPC, Research Institute for Animal Production in Nitra, Nitra, SlovakiaDepartment of Zoology and Anthropology, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, SlovakiaLaboratory of Animal Immunology and Biotechnology, Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech RepublicInstitute of Farm Animal Genetics and Reproduction, NPPC, Research Institute for Animal Production in Nitra, Nitra, Slovakia; Institute of Biotechnology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Nitra, Slovakia; Corresponding author:ABSTRACT: Mesenchymal stem cells (MSCs) are multilineage cells able to differentiate into other cell types. MSCs derived from bone marrow or compact bones are the most accessible stem cells used in tissue engineering. Therefore, the aim of this study was to isolate, characterize and cryopreserve MSCs of endangered Oravka chicken breed. MSCs were obtained from compact bones of the femur and tibiotarsus. MSCs were spindle-shaped and were able to differentiate into osteo-, adipo-, and chondrocytes under the specific differentiation conditions. Furthermore, MSCs were positive for surface markers such as CD29, CD44, CD73, CD90, CD105, CD146 and negative for CD34CD45 by flow cytometry. Moreover, MSCs demonstrated high positivity of “stemness” markers aldehyde dehydrogenase, alkaline phosphatase as well as for intracellular markers vimentin, desmin, α-SMA. Subsequently, MSCs were cryopreserved using 10% dimethyl sulfoxide in liquid nitrogen. Based on the results from the viability, phenotype, and ultrastructure assessment we can concluded that the MSCs were not negatively affected by the cryopreservation. Finally, MSCs of endangered Oravka chicken breed were successfully stored in animal gene bank, thus making them a valuable genetic resource.http://www.sciencedirect.com/science/article/pii/S0032579123003267chickennative breedstem cellflow cytometrycryopreservation |
| spellingShingle | Andrea Svoradová Jaromír Vašíček Vladimír Zmrhal Eva Venusová Aleš Pavlík Miroslav Bauer Lucia Olexiková Vladimír Langraf Petr Sláma Peter Chrenek Mesenchymal stem cells of Oravka chicken breed: promising path to biodiversity conservation Poultry Science chicken native breed stem cell flow cytometry cryopreservation |
| title | Mesenchymal stem cells of Oravka chicken breed: promising path to biodiversity conservation |
| title_full | Mesenchymal stem cells of Oravka chicken breed: promising path to biodiversity conservation |
| title_fullStr | Mesenchymal stem cells of Oravka chicken breed: promising path to biodiversity conservation |
| title_full_unstemmed | Mesenchymal stem cells of Oravka chicken breed: promising path to biodiversity conservation |
| title_short | Mesenchymal stem cells of Oravka chicken breed: promising path to biodiversity conservation |
| title_sort | mesenchymal stem cells of oravka chicken breed promising path to biodiversity conservation |
| topic | chicken native breed stem cell flow cytometry cryopreservation |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123003267 |
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