Perilipin overexpression in white adipose tissue induces a brown fat-like phenotype.
Perilipin A (PeriA) exclusively locates on adipocyte lipid droplets and is essential for lipid storage and lipolysis. Previously, we reported that adipocyte specific overexpression of PeriA caused resistance to diet-induced obesity and resulted in improved insulin sensitivity. In order to better und...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2010-11-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2982838?pdf=render |
| _version_ | 1818571506888736768 |
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| author | Takashi Sawada Hideaki Miyoshi Kohei Shimada Akira Suzuki Yuko Okamatsu-Ogura James W Perfield Takuma Kondo So Nagai Chikara Shimizu Narihito Yoshioka Andrew S Greenberg Kazuhiro Kimura Takao Koike |
| author_facet | Takashi Sawada Hideaki Miyoshi Kohei Shimada Akira Suzuki Yuko Okamatsu-Ogura James W Perfield Takuma Kondo So Nagai Chikara Shimizu Narihito Yoshioka Andrew S Greenberg Kazuhiro Kimura Takao Koike |
| author_sort | Takashi Sawada |
| collection | DOAJ |
| description | Perilipin A (PeriA) exclusively locates on adipocyte lipid droplets and is essential for lipid storage and lipolysis. Previously, we reported that adipocyte specific overexpression of PeriA caused resistance to diet-induced obesity and resulted in improved insulin sensitivity. In order to better understand the biological basis for this observed phenotype, we performed additional studies in this transgenic mouse model.When compared to control animals, whole body energy expenditure was increased in the transgenic mice. Subsequently, we performed DNA microarray analysis and real-time PCR on white adipose tissue. Consistent with the metabolic chamber data, we observed increased expression of genes associated with fatty acid β-oxidation and heat production, and a decrease in the genes associated with lipid synthesis. Gene expression of Pgc1a, a regulator of fatty acid oxidation and Ucp1, a brown adipocyte specific protein, was increased in the white adipose tissue of the transgenic mice. This observation was subsequently verified by both Western blotting and histological examination. Expression of RIP140, a regulator of white adipocyte differentiation, and the lipid droplet protein FSP27 was decreased in the transgenic mice. Importantly, FSP27 has been shown to control gene expression of these crucial metabolic regulators. Overexpression of PeriA in 3T3-L1 adipocytes also reduced FSP27 expression and diminished lipid droplet size.These findings demonstrate that overexpression of PeriA in white adipocytes reduces lipid droplet size by decreasing FSP27 expression and thereby inducing a brown adipose tissue-like phenotype. Our data suggest that modulation of lipid droplet proteins in white adipocytes is a potential therapeutic strategy for the treatment of obesity and its related disorders. |
| first_indexed | 2024-12-14T13:56:46Z |
| format | Article |
| id | doaj.art-cc2b7e41ccf14be9a990021e3db9af0f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T13:56:46Z |
| publishDate | 2010-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-cc2b7e41ccf14be9a990021e3db9af0f2022-12-21T22:58:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-11-01511e1400610.1371/journal.pone.0014006Perilipin overexpression in white adipose tissue induces a brown fat-like phenotype.Takashi SawadaHideaki MiyoshiKohei ShimadaAkira SuzukiYuko Okamatsu-OguraJames W PerfieldTakuma KondoSo NagaiChikara ShimizuNarihito YoshiokaAndrew S GreenbergKazuhiro KimuraTakao KoikePerilipin A (PeriA) exclusively locates on adipocyte lipid droplets and is essential for lipid storage and lipolysis. Previously, we reported that adipocyte specific overexpression of PeriA caused resistance to diet-induced obesity and resulted in improved insulin sensitivity. In order to better understand the biological basis for this observed phenotype, we performed additional studies in this transgenic mouse model.When compared to control animals, whole body energy expenditure was increased in the transgenic mice. Subsequently, we performed DNA microarray analysis and real-time PCR on white adipose tissue. Consistent with the metabolic chamber data, we observed increased expression of genes associated with fatty acid β-oxidation and heat production, and a decrease in the genes associated with lipid synthesis. Gene expression of Pgc1a, a regulator of fatty acid oxidation and Ucp1, a brown adipocyte specific protein, was increased in the white adipose tissue of the transgenic mice. This observation was subsequently verified by both Western blotting and histological examination. Expression of RIP140, a regulator of white adipocyte differentiation, and the lipid droplet protein FSP27 was decreased in the transgenic mice. Importantly, FSP27 has been shown to control gene expression of these crucial metabolic regulators. Overexpression of PeriA in 3T3-L1 adipocytes also reduced FSP27 expression and diminished lipid droplet size.These findings demonstrate that overexpression of PeriA in white adipocytes reduces lipid droplet size by decreasing FSP27 expression and thereby inducing a brown adipose tissue-like phenotype. Our data suggest that modulation of lipid droplet proteins in white adipocytes is a potential therapeutic strategy for the treatment of obesity and its related disorders.http://europepmc.org/articles/PMC2982838?pdf=render |
| spellingShingle | Takashi Sawada Hideaki Miyoshi Kohei Shimada Akira Suzuki Yuko Okamatsu-Ogura James W Perfield Takuma Kondo So Nagai Chikara Shimizu Narihito Yoshioka Andrew S Greenberg Kazuhiro Kimura Takao Koike Perilipin overexpression in white adipose tissue induces a brown fat-like phenotype. PLoS ONE |
| title | Perilipin overexpression in white adipose tissue induces a brown fat-like phenotype. |
| title_full | Perilipin overexpression in white adipose tissue induces a brown fat-like phenotype. |
| title_fullStr | Perilipin overexpression in white adipose tissue induces a brown fat-like phenotype. |
| title_full_unstemmed | Perilipin overexpression in white adipose tissue induces a brown fat-like phenotype. |
| title_short | Perilipin overexpression in white adipose tissue induces a brown fat-like phenotype. |
| title_sort | perilipin overexpression in white adipose tissue induces a brown fat like phenotype |
| url | http://europepmc.org/articles/PMC2982838?pdf=render |
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