Chronic Rhinosinusitis with Nasal Polyps: Age and Disease Severity Differences in the Levels of Inflammatory Markers
<i>Background and objectives:</i> The aim of our study was to analyze the concentrations of inflammatory markers in the nasal tissue of patients with chronic rhinosinusitis with nasal polyps (CRSwNPs) and controls of different age groups, as well as to find associations between age, infl...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/57/3/282 |
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| author | Justinas Vaitkus Astra Vitkauskienė Regimantas Simuntis Žygimantas Vaitkus Nora Šiupšinskienė Saulius Vaitkus |
| author_facet | Justinas Vaitkus Astra Vitkauskienė Regimantas Simuntis Žygimantas Vaitkus Nora Šiupšinskienė Saulius Vaitkus |
| author_sort | Justinas Vaitkus |
| collection | DOAJ |
| description | <i>Background and objectives:</i> The aim of our study was to analyze the concentrations of inflammatory markers in the nasal tissue of patients with chronic rhinosinusitis with nasal polyps (CRSwNPs) and controls of different age groups, as well as to find associations between age, inflammation development, and NPs. <i>Materials and methods</i>: Patients were divided into two groups—patients with CRSwNPs and control subjects who had nasal surgery for another reason beside CRS. Our analysis was performed across three different age groups (18–30 years, 31–50 years, and 51 years and more). Tissue biopsies from the sinus cavity for all study participants were taken and frozen at −80 °C, until use. The concentrations of IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-13, IL-21, and IL-22, were quantified using a magnetic bead-based multiplex assay. <i>Results</i>: In the group aged 18–30 years, the levels of inflammatory markers IL-1, IL-2, IL-5, and IL-22 were significantly higher in patients with CRSwNPs than the control subjects. Among patients aged 31–50 years, significantly higher concentrations of IL-2, IL-4, IL-5, and IL-22 were recorded in patients with CRSwNPs, as compared to the control subjects. In the oldest group (aged 51 years and more), patients with CRSwNPs had significantly higher concentrations of IL-2, IL-4, and IL-22, as compared to the control group. In the CRSwNP group, only the concentration of IL-21 was significantly higher among patients aged 31–50 years, as compared with those aged 51 years and older (<i>p</i> = 0.013). <i>Conclusions:</i> IL-2 and IL-22 levels were significantly higher in patients with CRSwNP than the control, across all age groups. Only the concentration of IL-21 was higher among patients with CRSwNP in the middle age group, as compared to the oldest group. IL-2, IL-4, and IL-22 levels correlated with the severity of CRSwNPs. Elevated concentrations of IL-2, IL-4, and IL-22 were determined in patients’ groups with higher sinonasal outcome test (SNOT-22) scores, pointing to more severe clinical symptoms. |
| first_indexed | 2024-03-10T13:08:08Z |
| format | Article |
| id | doaj.art-cc30d669d51f478286375b1d84324eba |
| institution | Directory Open Access Journal |
| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-03-10T13:08:08Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
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| series | Medicina |
| spelling | doaj.art-cc30d669d51f478286375b1d84324eba2023-11-21T10:57:47ZengMDPI AGMedicina1010-660X1648-91442021-03-0157328210.3390/medicina57030282Chronic Rhinosinusitis with Nasal Polyps: Age and Disease Severity Differences in the Levels of Inflammatory MarkersJustinas Vaitkus0Astra Vitkauskienė1Regimantas Simuntis2Žygimantas Vaitkus3Nora Šiupšinskienė4Saulius Vaitkus5Department of Otorhinolaryngology, Medicine Academy, Lithuanian University of Health Sciences, LT-50161 Kaunas, LithuaniaDepartment of Laboratory Medicine, Medicine Academy, Lithuanian University of Health Sciences, LT-50161 Kaunas, LithuaniaDepartment of Oral and Maxillofacial Surgery, Medicine Academy, Lithuanian University of Health Sciences, LT-50161 Kaunas, LithuaniaMedicine Academy, Lithuanian University of Health Sciences, LT-50161 Kaunas, LithuaniaDepartment of Otorhinolaryngology, Medicine Academy, Lithuanian University of Health Sciences, LT-50161 Kaunas, LithuaniaDepartment of Otorhinolaryngology, Medicine Academy, Lithuanian University of Health Sciences, LT-50161 Kaunas, Lithuania<i>Background and objectives:</i> The aim of our study was to analyze the concentrations of inflammatory markers in the nasal tissue of patients with chronic rhinosinusitis with nasal polyps (CRSwNPs) and controls of different age groups, as well as to find associations between age, inflammation development, and NPs. <i>Materials and methods</i>: Patients were divided into two groups—patients with CRSwNPs and control subjects who had nasal surgery for another reason beside CRS. Our analysis was performed across three different age groups (18–30 years, 31–50 years, and 51 years and more). Tissue biopsies from the sinus cavity for all study participants were taken and frozen at −80 °C, until use. The concentrations of IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-13, IL-21, and IL-22, were quantified using a magnetic bead-based multiplex assay. <i>Results</i>: In the group aged 18–30 years, the levels of inflammatory markers IL-1, IL-2, IL-5, and IL-22 were significantly higher in patients with CRSwNPs than the control subjects. Among patients aged 31–50 years, significantly higher concentrations of IL-2, IL-4, IL-5, and IL-22 were recorded in patients with CRSwNPs, as compared to the control subjects. In the oldest group (aged 51 years and more), patients with CRSwNPs had significantly higher concentrations of IL-2, IL-4, and IL-22, as compared to the control group. In the CRSwNP group, only the concentration of IL-21 was significantly higher among patients aged 31–50 years, as compared with those aged 51 years and older (<i>p</i> = 0.013). <i>Conclusions:</i> IL-2 and IL-22 levels were significantly higher in patients with CRSwNP than the control, across all age groups. Only the concentration of IL-21 was higher among patients with CRSwNP in the middle age group, as compared to the oldest group. IL-2, IL-4, and IL-22 levels correlated with the severity of CRSwNPs. Elevated concentrations of IL-2, IL-4, and IL-22 were determined in patients’ groups with higher sinonasal outcome test (SNOT-22) scores, pointing to more severe clinical symptoms.https://www.mdpi.com/1648-9144/57/3/282chronic rhinosinusitisnasal polypsseverity of diseaseageinflammatory markers |
| spellingShingle | Justinas Vaitkus Astra Vitkauskienė Regimantas Simuntis Žygimantas Vaitkus Nora Šiupšinskienė Saulius Vaitkus Chronic Rhinosinusitis with Nasal Polyps: Age and Disease Severity Differences in the Levels of Inflammatory Markers Medicina chronic rhinosinusitis nasal polyps severity of disease age inflammatory markers |
| title | Chronic Rhinosinusitis with Nasal Polyps: Age and Disease Severity Differences in the Levels of Inflammatory Markers |
| title_full | Chronic Rhinosinusitis with Nasal Polyps: Age and Disease Severity Differences in the Levels of Inflammatory Markers |
| title_fullStr | Chronic Rhinosinusitis with Nasal Polyps: Age and Disease Severity Differences in the Levels of Inflammatory Markers |
| title_full_unstemmed | Chronic Rhinosinusitis with Nasal Polyps: Age and Disease Severity Differences in the Levels of Inflammatory Markers |
| title_short | Chronic Rhinosinusitis with Nasal Polyps: Age and Disease Severity Differences in the Levels of Inflammatory Markers |
| title_sort | chronic rhinosinusitis with nasal polyps age and disease severity differences in the levels of inflammatory markers |
| topic | chronic rhinosinusitis nasal polyps severity of disease age inflammatory markers |
| url | https://www.mdpi.com/1648-9144/57/3/282 |
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