Streptococcus super antigen in polyp tissue of patients with nasal polyposis and chronic rhinosinusitis in comparison to normal population
Background: Nasal polyp (NP) is a benign mucosal mass located in both sinuses and nares which is mostly seen in association with cystic fibrosis, asthma or oversensitivity to aspirin. The prominent histological feature of NP is inflammatory cell infiltration with eosinophil predominance. Superantige...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Tehran University of Medical Sciences
2013-11-01
|
| Series: | Tehran University Medical Journal |
| Subjects: | |
| Online Access: | http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5102&slc_lang=en&sid=1 |
| _version_ | 1818172319367954432 |
|---|---|
| author | Mohammad Farhadi Mehdi Shekarabi Shima Javadinia Samileh Noorbaksh Mahmood Faramarzi Mohammad Reza shokrollahi Azardokht Tabatabaee |
| author_facet | Mohammad Farhadi Mehdi Shekarabi Shima Javadinia Samileh Noorbaksh Mahmood Faramarzi Mohammad Reza shokrollahi Azardokht Tabatabaee |
| author_sort | Mohammad Farhadi |
| collection | DOAJ |
| description | Background: Nasal polyp (NP) is a benign mucosal mass located in both sinuses and nares which is mostly seen in association with cystic fibrosis, asthma or oversensitivity to aspirin. The prominent histological feature of NP is inflammatory cell infiltration with eosinophil predominance. Superantigens role in causing NP complications is already proven. Superantigens, which are mostly originated from Streptococci and Staphylococci, activate T cells strongly and increase the process of production and release of cytokines, and secretion of IgE from B cells, which in turn directly affects proinflammatory cells such as eosinophils, both in their tissues infiltration and functions.Methods: The samples are collected from patients referring to ENT clinic in Rasoul Akram training Hospital in Tehran after thorough clinical and paraclinical examinations. For control group the samples collected from patients undergoing rhinoplasty. All the samples kept frozen and sent to immunology lab. The DNA of the excised tissues extracted and amplified by using the superantigens specific primers and PCR product detected by gel electrophoresis. The date analyzed by using mean and SD and χ2 analytical tools. Results: Fifteen healthy individuals, 25 patients with rhinosinusitis and 24 with polyposis entered this trial. Group A Streptococcus toxin detection was significantly more frequent in those with nasal polyp and rhinosinusitis compared to healthy individuals (P=0.001 and 0.005, respectively), but the results were almost the same for those with nasal polyp and rhinosinusitis (P=0.4).Conclusion: Streptococci may play an important role in induction or clinical exacerbation of polyposis and group A Streptococcus pyogenes exotoxin (SPEs) with superantigenic effects may have a crucial role in etiology and pathogenesis of polyps with or without rhinosinusitis. It is postulated that, T cells polyclonal activation by SPEs may cause recruitment of inflammatory cells in nasal mucosa. These inflammatory cells include IgE producing B cells laeding to allergic and inflammatory reactions in NP. |
| first_indexed | 2024-12-11T19:10:43Z |
| format | Article |
| id | doaj.art-cc31cf6735a841adbf660ace0cc52625 |
| institution | Directory Open Access Journal |
| issn | 1683-1764 1735-7322 |
| language | fas |
| last_indexed | 2024-12-11T19:10:43Z |
| publishDate | 2013-11-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Tehran University Medical Journal |
| spelling | doaj.art-cc31cf6735a841adbf660ace0cc526252022-12-22T00:53:47ZfasTehran University of Medical SciencesTehran University Medical Journal1683-17641735-73222013-11-01718524529Streptococcus super antigen in polyp tissue of patients with nasal polyposis and chronic rhinosinusitis in comparison to normal populationMohammad Farhadi0Mehdi Shekarabi1Shima Javadinia2Samileh Noorbaksh3Mahmood Faramarzi4Mohammad Reza shokrollahi5Azardokht Tabatabaee6 Department of ENT Research Center. Hazrat-e-Rasoul Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran. Department of Immunology, Faculty of Medicine Research Center of Pediatric Infectious Diseases. Hazrat-e-Rasoul Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran. Department of Internal Medicine, Research Center of Pediatric Infectious Diseases. Hazrat-e-Rasoul Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran. Department of Pediatric Infectious Disease, Research Center of Pediatric Infectious Diseases. Hazrat-e-Rasoul Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran. Department of Medical Immunology, Infectious Disease, Research Center of Pediatric Infectious Diseases. Hazrat-e-Rasoul Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran. Department of Pediatric Infectious Disease, Hazrat-e- Masoumeh Hospital, Ghom University of Medical Sciences, Ghom, Iran. Department of Laboratory Science, Instructor and Faculty member, Research Center of Pediatric Infectious Diseases. Hazrat-e-Rasoul Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran. Background: Nasal polyp (NP) is a benign mucosal mass located in both sinuses and nares which is mostly seen in association with cystic fibrosis, asthma or oversensitivity to aspirin. The prominent histological feature of NP is inflammatory cell infiltration with eosinophil predominance. Superantigens role in causing NP complications is already proven. Superantigens, which are mostly originated from Streptococci and Staphylococci, activate T cells strongly and increase the process of production and release of cytokines, and secretion of IgE from B cells, which in turn directly affects proinflammatory cells such as eosinophils, both in their tissues infiltration and functions.Methods: The samples are collected from patients referring to ENT clinic in Rasoul Akram training Hospital in Tehran after thorough clinical and paraclinical examinations. For control group the samples collected from patients undergoing rhinoplasty. All the samples kept frozen and sent to immunology lab. The DNA of the excised tissues extracted and amplified by using the superantigens specific primers and PCR product detected by gel electrophoresis. The date analyzed by using mean and SD and χ2 analytical tools. Results: Fifteen healthy individuals, 25 patients with rhinosinusitis and 24 with polyposis entered this trial. Group A Streptococcus toxin detection was significantly more frequent in those with nasal polyp and rhinosinusitis compared to healthy individuals (P=0.001 and 0.005, respectively), but the results were almost the same for those with nasal polyp and rhinosinusitis (P=0.4).Conclusion: Streptococci may play an important role in induction or clinical exacerbation of polyposis and group A Streptococcus pyogenes exotoxin (SPEs) with superantigenic effects may have a crucial role in etiology and pathogenesis of polyps with or without rhinosinusitis. It is postulated that, T cells polyclonal activation by SPEs may cause recruitment of inflammatory cells in nasal mucosa. These inflammatory cells include IgE producing B cells laeding to allergic and inflammatory reactions in NP.http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5102&slc_lang=en&sid=1chronic sinusitis nasal polyps streptococcus pyogenes superantigen |
| spellingShingle | Mohammad Farhadi Mehdi Shekarabi Shima Javadinia Samileh Noorbaksh Mahmood Faramarzi Mohammad Reza shokrollahi Azardokht Tabatabaee Streptococcus super antigen in polyp tissue of patients with nasal polyposis and chronic rhinosinusitis in comparison to normal population Tehran University Medical Journal chronic sinusitis nasal polyps streptococcus pyogenes superantigen |
| title | Streptococcus super antigen in polyp tissue of patients with nasal polyposis and chronic rhinosinusitis in comparison to normal population |
| title_full | Streptococcus super antigen in polyp tissue of patients with nasal polyposis and chronic rhinosinusitis in comparison to normal population |
| title_fullStr | Streptococcus super antigen in polyp tissue of patients with nasal polyposis and chronic rhinosinusitis in comparison to normal population |
| title_full_unstemmed | Streptococcus super antigen in polyp tissue of patients with nasal polyposis and chronic rhinosinusitis in comparison to normal population |
| title_short | Streptococcus super antigen in polyp tissue of patients with nasal polyposis and chronic rhinosinusitis in comparison to normal population |
| title_sort | streptococcus super antigen in polyp tissue of patients with nasal polyposis and chronic rhinosinusitis in comparison to normal population |
| topic | chronic sinusitis nasal polyps streptococcus pyogenes superantigen |
| url | http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5102&slc_lang=en&sid=1 |
| work_keys_str_mv | AT mohammadfarhadi streptococcussuperantigeninpolyptissueofpatientswithnasalpolyposisandchronicrhinosinusitisincomparisontonormalpopulation AT mehdishekarabi streptococcussuperantigeninpolyptissueofpatientswithnasalpolyposisandchronicrhinosinusitisincomparisontonormalpopulation AT shimajavadinia streptococcussuperantigeninpolyptissueofpatientswithnasalpolyposisandchronicrhinosinusitisincomparisontonormalpopulation AT samilehnoorbaksh streptococcussuperantigeninpolyptissueofpatientswithnasalpolyposisandchronicrhinosinusitisincomparisontonormalpopulation AT mahmoodfaramarzi streptococcussuperantigeninpolyptissueofpatientswithnasalpolyposisandchronicrhinosinusitisincomparisontonormalpopulation AT mohammadrezashokrollahi streptococcussuperantigeninpolyptissueofpatientswithnasalpolyposisandchronicrhinosinusitisincomparisontonormalpopulation AT azardokhttabatabaee streptococcussuperantigeninpolyptissueofpatientswithnasalpolyposisandchronicrhinosinusitisincomparisontonormalpopulation |