The Associated With Carbon Conversion Rate and Source–Sink Enzyme Activity in Tomato Fruit Subjected to Water Stress and Potassium Application
Carbon metabolism in higher plants is a basic physiological metabolism, and carbon allocation and conversion require the activity of various enzymes in metabolic processes that alter the content and overall composition of sugars in the sink organ. However, it is not known how various enzymes affect...
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| Format: | Article |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.681145/full |
| _version_ | 1818662169089146880 |
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| author | Anrong Luo Chenni Zhou Chenni Zhou Jinliang Chen |
| author_facet | Anrong Luo Chenni Zhou Chenni Zhou Jinliang Chen |
| author_sort | Anrong Luo |
| collection | DOAJ |
| description | Carbon metabolism in higher plants is a basic physiological metabolism, and carbon allocation and conversion require the activity of various enzymes in metabolic processes that alter the content and overall composition of sugars in the sink organ. However, it is not known how various enzymes affect carbon metabolism when tomato plants are subjected to water stress or treated with potassium. Although the process of carbon metabolism is very complex, we used the carbon conversion rate to compare and analyze the enzyme activities related to sugar metabolism and find out which carbon conversion rate are the most important. Results showed that water stress and potassium increased carbon import flux in the fruit, which was beneficial to carbon accumulation. Water deficit increased the activity of sucrose synthase (SuSy) and starch phosphorylase (SP) and decreased the activity of sucrose phosphate synthase (SPS) and adenosine diphosphate glucose pyrophosphorylase (AGPase) in the source. Water stress increased the activity of acid invertase (AI), SuSy and SP but decreased the activity of AGPase in the sink. Potassium modified the balance of enzymes active in sugar and starch metabolism by increasing the activity of AI, SuSy, SPS and SP and significantly decreasing the activity of AGPase, resulting in increase of hexose. Canonical correlational analysis revealed that the carbon conversion rate was mainly affected by the relative rate of conversion of sucrose to fructose and glucose [p1(t)] and glucose to starch [p5m(t)]. SuSy and AGPase had the greatest effect on enzyme activity in the fruit; respectively regulated p1(t) and p5m(t). |
| first_indexed | 2024-12-17T04:56:40Z |
| format | Article |
| id | doaj.art-cc35d3c2ca194a31988f123b6e5fe7f8 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-17T04:56:40Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-cc35d3c2ca194a31988f123b6e5fe7f82022-12-21T22:02:41ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-06-011210.3389/fpls.2021.681145681145The Associated With Carbon Conversion Rate and Source–Sink Enzyme Activity in Tomato Fruit Subjected to Water Stress and Potassium ApplicationAnrong Luo0Chenni Zhou1Chenni Zhou2Jinliang Chen3Center for Agricultural Water Research in China, China Agricultural University, Beijing, ChinaInstitute of Tibet Plateau Ecology, Tibet Agriculture and Animal Husbandry University, Nyingchi, ChinaKey Laboratory of Forest Ecology in Tibet Plateau (Tibet Agriculture and Animal Husbandry University), Ministry of Education, Nyingchi, ChinaCenter for Agricultural Water Research in China, China Agricultural University, Beijing, ChinaCarbon metabolism in higher plants is a basic physiological metabolism, and carbon allocation and conversion require the activity of various enzymes in metabolic processes that alter the content and overall composition of sugars in the sink organ. However, it is not known how various enzymes affect carbon metabolism when tomato plants are subjected to water stress or treated with potassium. Although the process of carbon metabolism is very complex, we used the carbon conversion rate to compare and analyze the enzyme activities related to sugar metabolism and find out which carbon conversion rate are the most important. Results showed that water stress and potassium increased carbon import flux in the fruit, which was beneficial to carbon accumulation. Water deficit increased the activity of sucrose synthase (SuSy) and starch phosphorylase (SP) and decreased the activity of sucrose phosphate synthase (SPS) and adenosine diphosphate glucose pyrophosphorylase (AGPase) in the source. Water stress increased the activity of acid invertase (AI), SuSy and SP but decreased the activity of AGPase in the sink. Potassium modified the balance of enzymes active in sugar and starch metabolism by increasing the activity of AI, SuSy, SPS and SP and significantly decreasing the activity of AGPase, resulting in increase of hexose. Canonical correlational analysis revealed that the carbon conversion rate was mainly affected by the relative rate of conversion of sucrose to fructose and glucose [p1(t)] and glucose to starch [p5m(t)]. SuSy and AGPase had the greatest effect on enzyme activity in the fruit; respectively regulated p1(t) and p5m(t).https://www.frontiersin.org/articles/10.3389/fpls.2021.681145/fullsucrose-metabolizing enzymesstarch metabolism enzymescarbon conversion ratetomatopotassiumwater stress |
| spellingShingle | Anrong Luo Chenni Zhou Chenni Zhou Jinliang Chen The Associated With Carbon Conversion Rate and Source–Sink Enzyme Activity in Tomato Fruit Subjected to Water Stress and Potassium Application Frontiers in Plant Science sucrose-metabolizing enzymes starch metabolism enzymes carbon conversion rate tomato potassium water stress |
| title | The Associated With Carbon Conversion Rate and Source–Sink Enzyme Activity in Tomato Fruit Subjected to Water Stress and Potassium Application |
| title_full | The Associated With Carbon Conversion Rate and Source–Sink Enzyme Activity in Tomato Fruit Subjected to Water Stress and Potassium Application |
| title_fullStr | The Associated With Carbon Conversion Rate and Source–Sink Enzyme Activity in Tomato Fruit Subjected to Water Stress and Potassium Application |
| title_full_unstemmed | The Associated With Carbon Conversion Rate and Source–Sink Enzyme Activity in Tomato Fruit Subjected to Water Stress and Potassium Application |
| title_short | The Associated With Carbon Conversion Rate and Source–Sink Enzyme Activity in Tomato Fruit Subjected to Water Stress and Potassium Application |
| title_sort | associated with carbon conversion rate and source sink enzyme activity in tomato fruit subjected to water stress and potassium application |
| topic | sucrose-metabolizing enzymes starch metabolism enzymes carbon conversion rate tomato potassium water stress |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.681145/full |
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