Efficient use of discarded vegetal residues as cost-effective feedstocks for microbial oil production
Abstract Background Horticultural intensive type systems dedicated in producing greenhouse vegetables are one of the primary industries generating organic waste. Towards the implementation of a zero-waste strategy, this work aims to use discarded vegetables (tomato, pepper and watermelon) as feedsto...
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-023-02268-5 |
| _version_ | 1811166026584293376 |
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| author | María Gallego-García Antonio D. Moreno Alberto González María José Negro |
| author_facet | María Gallego-García Antonio D. Moreno Alberto González María José Negro |
| author_sort | María Gallego-García |
| collection | DOAJ |
| description | Abstract Background Horticultural intensive type systems dedicated in producing greenhouse vegetables are one of the primary industries generating organic waste. Towards the implementation of a zero-waste strategy, this work aims to use discarded vegetables (tomato, pepper and watermelon) as feedstock for producing microbial oil using the oleaginous yeast Cryptococcus curvatus. Results The soluble fraction, resulting after crushing and centrifuging these residues, showed C/N ratios of about 15, with a total carbohydrate content (mainly glucose, fructose and sucrose) ranging from 30 g/L to 65 g/L. Using these liquid fractions as substrate under a pulse-feeding strategy with a concentrated glucose solution resulted in an intracellular total lipid accumulation of about 30% (w/w) of the total dry cell weight (DCW). To increase this intracellular lipid content, the initial C/N content was increased from 15 to 30 and 50. Under these conditions, the process performance of the pulse-feeding strategy increased by 20–36%, resulting in a total intracellular lipid concentration of 35–40% DCW (w/w). Conclusion These results demonstrate the potential of discarded vegetables as a substrate for producing bio-based products such as microbial oil when proper cultivation strategies are available. |
| first_indexed | 2024-04-10T15:46:49Z |
| format | Article |
| id | doaj.art-cc3634f0c6a645d0864d2c947810807f |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-04-10T15:46:49Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-cc3634f0c6a645d0864d2c947810807f2023-02-12T12:06:43ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542023-02-0116111110.1186/s13068-023-02268-5Efficient use of discarded vegetal residues as cost-effective feedstocks for microbial oil productionMaría Gallego-García0Antonio D. Moreno1Alberto González2María José Negro3Advanced Biofuels and Bioproducts Unit, CIEMATAdvanced Biofuels and Bioproducts Unit, CIEMATAdvanced Biofuels and Bioproducts Unit, CIEMATAdvanced Biofuels and Bioproducts Unit, CIEMATAbstract Background Horticultural intensive type systems dedicated in producing greenhouse vegetables are one of the primary industries generating organic waste. Towards the implementation of a zero-waste strategy, this work aims to use discarded vegetables (tomato, pepper and watermelon) as feedstock for producing microbial oil using the oleaginous yeast Cryptococcus curvatus. Results The soluble fraction, resulting after crushing and centrifuging these residues, showed C/N ratios of about 15, with a total carbohydrate content (mainly glucose, fructose and sucrose) ranging from 30 g/L to 65 g/L. Using these liquid fractions as substrate under a pulse-feeding strategy with a concentrated glucose solution resulted in an intracellular total lipid accumulation of about 30% (w/w) of the total dry cell weight (DCW). To increase this intracellular lipid content, the initial C/N content was increased from 15 to 30 and 50. Under these conditions, the process performance of the pulse-feeding strategy increased by 20–36%, resulting in a total intracellular lipid concentration of 35–40% DCW (w/w). Conclusion These results demonstrate the potential of discarded vegetables as a substrate for producing bio-based products such as microbial oil when proper cultivation strategies are available.https://doi.org/10.1186/s13068-023-02268-5Single cell oilAgri-food residuesOleaginous yeastLipids |
| spellingShingle | María Gallego-García Antonio D. Moreno Alberto González María José Negro Efficient use of discarded vegetal residues as cost-effective feedstocks for microbial oil production Biotechnology for Biofuels and Bioproducts Single cell oil Agri-food residues Oleaginous yeast Lipids |
| title | Efficient use of discarded vegetal residues as cost-effective feedstocks for microbial oil production |
| title_full | Efficient use of discarded vegetal residues as cost-effective feedstocks for microbial oil production |
| title_fullStr | Efficient use of discarded vegetal residues as cost-effective feedstocks for microbial oil production |
| title_full_unstemmed | Efficient use of discarded vegetal residues as cost-effective feedstocks for microbial oil production |
| title_short | Efficient use of discarded vegetal residues as cost-effective feedstocks for microbial oil production |
| title_sort | efficient use of discarded vegetal residues as cost effective feedstocks for microbial oil production |
| topic | Single cell oil Agri-food residues Oleaginous yeast Lipids |
| url | https://doi.org/10.1186/s13068-023-02268-5 |
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