Dispersive biofilm from membrane bioreactor strains: effects of diffusible signal factor addition and characterization by dispersion index
IntroductionBiofilm occurs ubiquitously in water system. Excessive biofilm formation deteriorates severely system performance in several water and wastewater treatment processes. Quorum sensing systems were controlled in this study with a signal compound cis-2-Decenoic acid (CDA) to regulate various...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1211761/full |
| _version_ | 1797772964758814720 |
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| author | Wonjung Song Junhee Ryu Jaehyun Jung Youngjae Yu Suyoung Choi Jihyang Kweon |
| author_facet | Wonjung Song Junhee Ryu Jaehyun Jung Youngjae Yu Suyoung Choi Jihyang Kweon |
| author_sort | Wonjung Song |
| collection | DOAJ |
| description | IntroductionBiofilm occurs ubiquitously in water system. Excessive biofilm formation deteriorates severely system performance in several water and wastewater treatment processes. Quorum sensing systems were controlled in this study with a signal compound cis-2-Decenoic acid (CDA) to regulate various functions of microbial communities, including motility, enzyme production, and extracellular polymeric substance (EPS) production in biofilm.MethodsThe addition of CDA to six strains extracted from membrane bioreactor sludge and the Pseudomonas aeruginosa PAO1 strain was examined for modulating biofilm development by regulating DSF expression.Results and discussionAs the CDA doses increased, optical density of the biofilm dispersion assay increased, and the decrease in EPS of the biofilm was obvious on membrane surfaces. The three-dimensional visual images and quantitative analyses of biofilm formation with CDA proved thinner, less massive, and more dispersive than those without; to evaluate its dispersive intensity, a dispersion index was proposed. This could compare the dispersive effects of CDA dosing to other biofilms or efficiencies of biofouling control practices such as backwashing or new cleaning methods. |
| first_indexed | 2024-03-12T21:59:25Z |
| format | Article |
| id | doaj.art-cc379dc605114fdeabc235d4926f93d4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T21:59:25Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-cc379dc605114fdeabc235d4926f93d42023-07-25T10:42:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-07-011410.3389/fmicb.2023.12117611211761Dispersive biofilm from membrane bioreactor strains: effects of diffusible signal factor addition and characterization by dispersion indexWonjung Song0Junhee Ryu1Jaehyun Jung2Youngjae Yu3Suyoung Choi4Jihyang Kweon5The Academy of Applied Science and Technology, Konkuk University, Seoul, Republic of KoreaDepartment of Civil and Environmental Engineering, Konkuk University, Seoul, Republic of KoreaHANSU Technical Service Ltd, Sungnam-si, Gyeonggi-do, Republic of KoreaDepartment of Chemical and Environmental Engineering, University of Arizona, Tucson, AZ, United StatesThe Academy of Applied Science and Technology, Konkuk University, Seoul, Republic of KoreaDepartment of Environmental Engineering Konkuk University, Seoul, Republic of KoreaIntroductionBiofilm occurs ubiquitously in water system. Excessive biofilm formation deteriorates severely system performance in several water and wastewater treatment processes. Quorum sensing systems were controlled in this study with a signal compound cis-2-Decenoic acid (CDA) to regulate various functions of microbial communities, including motility, enzyme production, and extracellular polymeric substance (EPS) production in biofilm.MethodsThe addition of CDA to six strains extracted from membrane bioreactor sludge and the Pseudomonas aeruginosa PAO1 strain was examined for modulating biofilm development by regulating DSF expression.Results and discussionAs the CDA doses increased, optical density of the biofilm dispersion assay increased, and the decrease in EPS of the biofilm was obvious on membrane surfaces. The three-dimensional visual images and quantitative analyses of biofilm formation with CDA proved thinner, less massive, and more dispersive than those without; to evaluate its dispersive intensity, a dispersion index was proposed. This could compare the dispersive effects of CDA dosing to other biofilms or efficiencies of biofouling control practices such as backwashing or new cleaning methods.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1211761/fullmembrane bioreactorcis-2-Decenoic aciddiffusible signal factor (DSF)quorum sensingbiofilmextracellular polymeric substances (EPS) |
| spellingShingle | Wonjung Song Junhee Ryu Jaehyun Jung Youngjae Yu Suyoung Choi Jihyang Kweon Dispersive biofilm from membrane bioreactor strains: effects of diffusible signal factor addition and characterization by dispersion index Frontiers in Microbiology membrane bioreactor cis-2-Decenoic acid diffusible signal factor (DSF) quorum sensing biofilm extracellular polymeric substances (EPS) |
| title | Dispersive biofilm from membrane bioreactor strains: effects of diffusible signal factor addition and characterization by dispersion index |
| title_full | Dispersive biofilm from membrane bioreactor strains: effects of diffusible signal factor addition and characterization by dispersion index |
| title_fullStr | Dispersive biofilm from membrane bioreactor strains: effects of diffusible signal factor addition and characterization by dispersion index |
| title_full_unstemmed | Dispersive biofilm from membrane bioreactor strains: effects of diffusible signal factor addition and characterization by dispersion index |
| title_short | Dispersive biofilm from membrane bioreactor strains: effects of diffusible signal factor addition and characterization by dispersion index |
| title_sort | dispersive biofilm from membrane bioreactor strains effects of diffusible signal factor addition and characterization by dispersion index |
| topic | membrane bioreactor cis-2-Decenoic acid diffusible signal factor (DSF) quorum sensing biofilm extracellular polymeric substances (EPS) |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1211761/full |
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