Cytotoxicity Evaluation of Photosensitizer-Conjugated Hexagonal Upconverting Nanoparticles
In this report, we synthesized hexagonal NaYF<sub>4</sub>:Yb,Er upconverting nanoparticles (UCNPs) of 171 nm in size with a narrow particle size distribution. To address their colloidal stabi-lity in aqueous media and to incorporate a photosensitizer that can produce reactive singlet oxy...
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| Materialtyp: | Artikel |
| Språk: | English |
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MDPI AG
2023-05-01
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| Serie: | Nanomaterials |
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| Länkar: | https://www.mdpi.com/2079-4991/13/9/1535 |
| _version_ | 1827742748907667456 |
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| author | Mykhailo Nahorniak Viktoriia Oleksa Taras Vasylyshyn Ognen Pop-Georgievski Eliška Rydvalová Marcela Filipová Daniel Horák |
| author_facet | Mykhailo Nahorniak Viktoriia Oleksa Taras Vasylyshyn Ognen Pop-Georgievski Eliška Rydvalová Marcela Filipová Daniel Horák |
| author_sort | Mykhailo Nahorniak |
| collection | DOAJ |
| description | In this report, we synthesized hexagonal NaYF<sub>4</sub>:Yb,Er upconverting nanoparticles (UCNPs) of 171 nm in size with a narrow particle size distribution. To address their colloidal stabi-lity in aqueous media and to incorporate a photosensitizer that can produce reactive singlet oxygen (<sup>1</sup>O<sub>2</sub>) to kill tumor cells, UCNPs were conjugated with 6-bromohexanoic acid-functionalized Rose Bengal (RB) and coated with PEG-alendronate (PEG-Ale). The particles were thoroughly characterized by transmission electron microscopy, dynamic light scattering, ATR FTIR, X-ray photoelectron spectroscopy, thermogravimetric analysis, and spectrofluorometry, and <sup>1</sup>O<sub>2</sub> formation was detected using a 9,10-diphenylanthracene spectrophotometric probe. Cytotoxicity determination on rat mesenchymal stem cells by using the MTT assay showed that neutralization of the large positive surface charge of neat UCNPs with PEG-Ale and the bound RB sensitizer significantly reduced the concentration-dependent cytotoxicity. The presented strategy shows great potential for the use of these particles as a novel agent for the photodynamic therapy of tumors. |
| first_indexed | 2024-03-11T04:11:33Z |
| format | Article |
| id | doaj.art-cc39a8c6353143ada3c99d877e95b6c3 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-11T04:11:33Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-cc39a8c6353143ada3c99d877e95b6c32023-11-17T23:27:22ZengMDPI AGNanomaterials2079-49912023-05-01139153510.3390/nano13091535Cytotoxicity Evaluation of Photosensitizer-Conjugated Hexagonal Upconverting NanoparticlesMykhailo Nahorniak0Viktoriia Oleksa1Taras Vasylyshyn2Ognen Pop-Georgievski3Eliška Rydvalová4Marcela Filipová5Daniel Horák6Institute of Macromolecular Chemistry, Czech Academy of Sciences, 160 00 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, 160 00 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, 160 00 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, 160 00 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, 160 00 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, 160 00 Prague, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, 160 00 Prague, Czech RepublicIn this report, we synthesized hexagonal NaYF<sub>4</sub>:Yb,Er upconverting nanoparticles (UCNPs) of 171 nm in size with a narrow particle size distribution. To address their colloidal stabi-lity in aqueous media and to incorporate a photosensitizer that can produce reactive singlet oxygen (<sup>1</sup>O<sub>2</sub>) to kill tumor cells, UCNPs were conjugated with 6-bromohexanoic acid-functionalized Rose Bengal (RB) and coated with PEG-alendronate (PEG-Ale). The particles were thoroughly characterized by transmission electron microscopy, dynamic light scattering, ATR FTIR, X-ray photoelectron spectroscopy, thermogravimetric analysis, and spectrofluorometry, and <sup>1</sup>O<sub>2</sub> formation was detected using a 9,10-diphenylanthracene spectrophotometric probe. Cytotoxicity determination on rat mesenchymal stem cells by using the MTT assay showed that neutralization of the large positive surface charge of neat UCNPs with PEG-Ale and the bound RB sensitizer significantly reduced the concentration-dependent cytotoxicity. The presented strategy shows great potential for the use of these particles as a novel agent for the photodynamic therapy of tumors.https://www.mdpi.com/2079-4991/13/9/1535upcovertingnanoparticlesrose bengalphotosensitizercytotoxicity |
| spellingShingle | Mykhailo Nahorniak Viktoriia Oleksa Taras Vasylyshyn Ognen Pop-Georgievski Eliška Rydvalová Marcela Filipová Daniel Horák Cytotoxicity Evaluation of Photosensitizer-Conjugated Hexagonal Upconverting Nanoparticles Nanomaterials upcoverting nanoparticles rose bengal photosensitizer cytotoxicity |
| title | Cytotoxicity Evaluation of Photosensitizer-Conjugated Hexagonal Upconverting Nanoparticles |
| title_full | Cytotoxicity Evaluation of Photosensitizer-Conjugated Hexagonal Upconverting Nanoparticles |
| title_fullStr | Cytotoxicity Evaluation of Photosensitizer-Conjugated Hexagonal Upconverting Nanoparticles |
| title_full_unstemmed | Cytotoxicity Evaluation of Photosensitizer-Conjugated Hexagonal Upconverting Nanoparticles |
| title_short | Cytotoxicity Evaluation of Photosensitizer-Conjugated Hexagonal Upconverting Nanoparticles |
| title_sort | cytotoxicity evaluation of photosensitizer conjugated hexagonal upconverting nanoparticles |
| topic | upcoverting nanoparticles rose bengal photosensitizer cytotoxicity |
| url | https://www.mdpi.com/2079-4991/13/9/1535 |
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