Production and biochemical characterization of keratinase enzyme from Bacillus subtilis ES5 and its potential application in leather dehairing process: a clean leather tanning process
AbstractThe traditional leather industry releases different chemical wastes into the environment, which calls for the use of alternative, nonpolluting processes. The use of biological catalysts in industrial processing has received a great deal of attention in recent years, mainly for environmental...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2023.2288691 |
| _version_ | 1797367499982897152 |
|---|---|
| author | Getachew Alamnie Amare Gessesse Misganaw Bitew Nebiyu Dawud Berhanu Andualem Abayeneh Girma |
| author_facet | Getachew Alamnie Amare Gessesse Misganaw Bitew Nebiyu Dawud Berhanu Andualem Abayeneh Girma |
| author_sort | Getachew Alamnie |
| collection | DOAJ |
| description | AbstractThe traditional leather industry releases different chemical wastes into the environment, which calls for the use of alternative, nonpolluting processes. The use of biological catalysts in industrial processing has received a great deal of attention in recent years, mainly for environmental reasons. The main objective of this study was to produce and characterize keratinase enzyme from Bacillus subtilis ES5 and evaluate its potential application for leather dehairing. The gene encoding keratinase was validated with a fragment length of approximately 1100 bp. The keratinase exhibited maximum activity at temperature and pH of 45 °C and 8, respectively. Among the tested metal ions, Ca2+ and Mg2+ improved keratinase activity by 132.56 and 111.78%, respectively, at concentrations of 5 mM. β-mercaptoethanol enhanced keratinolytic activity. However, ethylenediaminetetraacetic acid (EDTA) significantly inhibited it, indicating that the enzyme is a member of the metalloprotease family. It completely dehaired goat/sheep skins within 14 h, resulting in decreased biochemical oxygen demand (BOD5), chemical oxygen demand (COD), total dissolved solids (TDS), total suspended solids (TSS), and overall pollution load by 57.39, 70.53, 93.59, 94.90, and 79.10%, respectively. The results demonstrated the pivotal advantages of this keratinase, addressing environmentally friendly processing and sustainable solid waste management. The improved properties of enzyme-treated dehaired pelts suggest that crude keratinase could be used in ecologically friendly holistic leather manufacturing to avoid the pollution issues associated with the use of chemicals. |
| first_indexed | 2024-03-08T17:19:12Z |
| format | Article |
| id | doaj.art-cc3a9507c2e14dbdad04e1db8f34d1c5 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-03-08T17:19:12Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-cc3a9507c2e14dbdad04e1db8f34d1c52024-01-03T09:44:12ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302023-12-0137110.1080/13102818.2023.2288691Production and biochemical characterization of keratinase enzyme from Bacillus subtilis ES5 and its potential application in leather dehairing process: a clean leather tanning processGetachew Alamnie0Amare Gessesse1Misganaw Bitew2Nebiyu Dawud3Berhanu Andualem4Abayeneh Girma5Department of Biological Sciences, College of Natural and Computational Science, Mekdela Amba University, Tuluawliya, EthiopiaDepartment of Biological Sciences and Biotechnology, Faculty of Sciences, Botswana International University of Science and Technology, Palapye, BotswanaBahir Dar Tannery Industry, Bahir Dar, EthiopiaBahir Dar Tannery Industry, Bahir Dar, EthiopiaDepartment of Industrial and Environmental Biotechnology, Institute of Biotechnology, University of Gondar, Gondar, EthiopiaDepartment of Biological Sciences, College of Natural and Computational Science, Mekdela Amba University, Tuluawliya, EthiopiaAbstractThe traditional leather industry releases different chemical wastes into the environment, which calls for the use of alternative, nonpolluting processes. The use of biological catalysts in industrial processing has received a great deal of attention in recent years, mainly for environmental reasons. The main objective of this study was to produce and characterize keratinase enzyme from Bacillus subtilis ES5 and evaluate its potential application for leather dehairing. The gene encoding keratinase was validated with a fragment length of approximately 1100 bp. The keratinase exhibited maximum activity at temperature and pH of 45 °C and 8, respectively. Among the tested metal ions, Ca2+ and Mg2+ improved keratinase activity by 132.56 and 111.78%, respectively, at concentrations of 5 mM. β-mercaptoethanol enhanced keratinolytic activity. However, ethylenediaminetetraacetic acid (EDTA) significantly inhibited it, indicating that the enzyme is a member of the metalloprotease family. It completely dehaired goat/sheep skins within 14 h, resulting in decreased biochemical oxygen demand (BOD5), chemical oxygen demand (COD), total dissolved solids (TDS), total suspended solids (TSS), and overall pollution load by 57.39, 70.53, 93.59, 94.90, and 79.10%, respectively. The results demonstrated the pivotal advantages of this keratinase, addressing environmentally friendly processing and sustainable solid waste management. The improved properties of enzyme-treated dehaired pelts suggest that crude keratinase could be used in ecologically friendly holistic leather manufacturing to avoid the pollution issues associated with the use of chemicals.https://www.tandfonline.com/doi/10.1080/13102818.2023.2288691Keratinasedehairingleathereffluent analysischaracterizationenzyme production |
| spellingShingle | Getachew Alamnie Amare Gessesse Misganaw Bitew Nebiyu Dawud Berhanu Andualem Abayeneh Girma Production and biochemical characterization of keratinase enzyme from Bacillus subtilis ES5 and its potential application in leather dehairing process: a clean leather tanning process Biotechnology & Biotechnological Equipment Keratinase dehairing leather effluent analysis characterization enzyme production |
| title | Production and biochemical characterization of keratinase enzyme from Bacillus subtilis ES5 and its potential application in leather dehairing process: a clean leather tanning process |
| title_full | Production and biochemical characterization of keratinase enzyme from Bacillus subtilis ES5 and its potential application in leather dehairing process: a clean leather tanning process |
| title_fullStr | Production and biochemical characterization of keratinase enzyme from Bacillus subtilis ES5 and its potential application in leather dehairing process: a clean leather tanning process |
| title_full_unstemmed | Production and biochemical characterization of keratinase enzyme from Bacillus subtilis ES5 and its potential application in leather dehairing process: a clean leather tanning process |
| title_short | Production and biochemical characterization of keratinase enzyme from Bacillus subtilis ES5 and its potential application in leather dehairing process: a clean leather tanning process |
| title_sort | production and biochemical characterization of keratinase enzyme from bacillus subtilis es5 and its potential application in leather dehairing process a clean leather tanning process |
| topic | Keratinase dehairing leather effluent analysis characterization enzyme production |
| url | https://www.tandfonline.com/doi/10.1080/13102818.2023.2288691 |
| work_keys_str_mv | AT getachewalamnie productionandbiochemicalcharacterizationofkeratinaseenzymefrombacillussubtilises5anditspotentialapplicationinleatherdehairingprocessacleanleathertanningprocess AT amaregessesse productionandbiochemicalcharacterizationofkeratinaseenzymefrombacillussubtilises5anditspotentialapplicationinleatherdehairingprocessacleanleathertanningprocess AT misganawbitew productionandbiochemicalcharacterizationofkeratinaseenzymefrombacillussubtilises5anditspotentialapplicationinleatherdehairingprocessacleanleathertanningprocess AT nebiyudawud productionandbiochemicalcharacterizationofkeratinaseenzymefrombacillussubtilises5anditspotentialapplicationinleatherdehairingprocessacleanleathertanningprocess AT berhanuandualem productionandbiochemicalcharacterizationofkeratinaseenzymefrombacillussubtilises5anditspotentialapplicationinleatherdehairingprocessacleanleathertanningprocess AT abayenehgirma productionandbiochemicalcharacterizationofkeratinaseenzymefrombacillussubtilises5anditspotentialapplicationinleatherdehairingprocessacleanleathertanningprocess |