Anti-Osteoporosis Effect of <i>Perilla frutescens</i> Leaf Hexane Fraction through Regulating Osteoclast and Osteoblast Differentiation
Osteoporosis is the result of an imbalance in the bone-remodeling process via an increase in osteoclastic activity and a decrease in osteoblastic activity. Our previous studies have shown that <i>Perilla frutescens</i> seed meal has anti-osteoclastogenic activity. However, the role of pe...
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MDPI AG
2022-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/3/824 |
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| author | Kanokkarn Phromnoi Supachai Yodkeeree Komsak Pintha Sariya Mapoung Maitree Suttajit Chalermpong Saenjum Pornngarm Dejkriengkraikul |
| author_facet | Kanokkarn Phromnoi Supachai Yodkeeree Komsak Pintha Sariya Mapoung Maitree Suttajit Chalermpong Saenjum Pornngarm Dejkriengkraikul |
| author_sort | Kanokkarn Phromnoi |
| collection | DOAJ |
| description | Osteoporosis is the result of an imbalance in the bone-remodeling process via an increase in osteoclastic activity and a decrease in osteoblastic activity. Our previous studies have shown that <i>Perilla frutescens</i> seed meal has anti-osteoclastogenic activity. However, the role of perilla leaf hexane fraction (PLH) in osteoporosis has not yet been investigated and reported. In this study, we aimed to investigate the effects of PLH in osteoclast differentiation and osteogenic potential using cell-based experiments in vitro. From HPLC analysis, we found that PLH contained high luteolin and baicalein. PLH was shown to inhibit RANKL-induced ROS production and tartrate-resistant acid phosphatase (TRAP)-positive multi-nucleated osteoclasts. Moreover, PLH significantly downregulated the RANKL-induced MAPK and NF-κB signaling pathways, leading to the attenuation of NFATc1 and MMP-9 expression. In contrast, PLH enhanced osteoblast function by regulating alkaline phosphatase (ALP) and restoring TNF-α-suppressed osteoblast proliferation and osteogenic potential. Thus, luteolin and baicalein-rich PLH inhibits osteoclast differentiation but promotes the function of osteoblasts. Collectively, our data provide new evidence that suggests that PLH may be a valuable anti-osteoporosis agent. |
| first_indexed | 2024-03-09T23:29:38Z |
| format | Article |
| id | doaj.art-cc3a979b77af4c75a28c909c8c592d4e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:29:38Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-cc3a979b77af4c75a28c909c8c592d4e2023-11-23T17:13:00ZengMDPI AGMolecules1420-30492022-01-0127382410.3390/molecules27030824Anti-Osteoporosis Effect of <i>Perilla frutescens</i> Leaf Hexane Fraction through Regulating Osteoclast and Osteoblast DifferentiationKanokkarn Phromnoi0Supachai Yodkeeree1Komsak Pintha2Sariya Mapoung3Maitree Suttajit4Chalermpong Saenjum5Pornngarm Dejkriengkraikul6Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao 56000, ThailandDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Biochemistry, School of Medical Sciences, University of Phayao, Phayao 56000, ThailandDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Biochemistry, School of Medical Sciences, University of Phayao, Phayao 56000, ThailandDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandOsteoporosis is the result of an imbalance in the bone-remodeling process via an increase in osteoclastic activity and a decrease in osteoblastic activity. Our previous studies have shown that <i>Perilla frutescens</i> seed meal has anti-osteoclastogenic activity. However, the role of perilla leaf hexane fraction (PLH) in osteoporosis has not yet been investigated and reported. In this study, we aimed to investigate the effects of PLH in osteoclast differentiation and osteogenic potential using cell-based experiments in vitro. From HPLC analysis, we found that PLH contained high luteolin and baicalein. PLH was shown to inhibit RANKL-induced ROS production and tartrate-resistant acid phosphatase (TRAP)-positive multi-nucleated osteoclasts. Moreover, PLH significantly downregulated the RANKL-induced MAPK and NF-κB signaling pathways, leading to the attenuation of NFATc1 and MMP-9 expression. In contrast, PLH enhanced osteoblast function by regulating alkaline phosphatase (ALP) and restoring TNF-α-suppressed osteoblast proliferation and osteogenic potential. Thus, luteolin and baicalein-rich PLH inhibits osteoclast differentiation but promotes the function of osteoblasts. Collectively, our data provide new evidence that suggests that PLH may be a valuable anti-osteoporosis agent.https://www.mdpi.com/1420-3049/27/3/824<i>Perilla frutescens</i>osteoporosisosteoclastosteoblastRANKLTNF-α |
| spellingShingle | Kanokkarn Phromnoi Supachai Yodkeeree Komsak Pintha Sariya Mapoung Maitree Suttajit Chalermpong Saenjum Pornngarm Dejkriengkraikul Anti-Osteoporosis Effect of <i>Perilla frutescens</i> Leaf Hexane Fraction through Regulating Osteoclast and Osteoblast Differentiation Molecules <i>Perilla frutescens</i> osteoporosis osteoclast osteoblast RANKL TNF-α |
| title | Anti-Osteoporosis Effect of <i>Perilla frutescens</i> Leaf Hexane Fraction through Regulating Osteoclast and Osteoblast Differentiation |
| title_full | Anti-Osteoporosis Effect of <i>Perilla frutescens</i> Leaf Hexane Fraction through Regulating Osteoclast and Osteoblast Differentiation |
| title_fullStr | Anti-Osteoporosis Effect of <i>Perilla frutescens</i> Leaf Hexane Fraction through Regulating Osteoclast and Osteoblast Differentiation |
| title_full_unstemmed | Anti-Osteoporosis Effect of <i>Perilla frutescens</i> Leaf Hexane Fraction through Regulating Osteoclast and Osteoblast Differentiation |
| title_short | Anti-Osteoporosis Effect of <i>Perilla frutescens</i> Leaf Hexane Fraction through Regulating Osteoclast and Osteoblast Differentiation |
| title_sort | anti osteoporosis effect of i perilla frutescens i leaf hexane fraction through regulating osteoclast and osteoblast differentiation |
| topic | <i>Perilla frutescens</i> osteoporosis osteoclast osteoblast RANKL TNF-α |
| url | https://www.mdpi.com/1420-3049/27/3/824 |
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