In Vitro Comparative Study of Platelets Treated with Two Pathogen-Inactivation Methods to Extend Shelf Life to 7 Days
Background and Objectives: Since 2015, platelet products have been pathogen-inactivated (PI) at the Luxemburgish Red Cross (LRC) using Riboflavin and UV light (RF-PI). As the LRC should respond to hospital needs at any time, platelet production exceeds the demand, generating a discard rate of 18%. T...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/3/343 |
| _version_ | 1797443795522945024 |
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| author | Nicolas Malvaux Fanette Defraigne Styliani Bartziali Camille Bellora Kathleen Mommaerts Fay Betsou Anne Schuhmacher |
| author_facet | Nicolas Malvaux Fanette Defraigne Styliani Bartziali Camille Bellora Kathleen Mommaerts Fay Betsou Anne Schuhmacher |
| author_sort | Nicolas Malvaux |
| collection | DOAJ |
| description | Background and Objectives: Since 2015, platelet products have been pathogen-inactivated (PI) at the Luxemburgish Red Cross (LRC) using Riboflavin and UV light (RF-PI). As the LRC should respond to hospital needs at any time, platelet production exceeds the demand, generating a discard rate of 18%. To reduce this, we consider the extension of storage time from 5 to 7 days. This study’s objective was to evaluate the in vitro 7-day platelet-storage quality, comparing two PI technologies, RF-PI and amotosalen/UVA light (AM-PI), for platelet pools from whole-blood donations (PPCs) and apheresis platelets collected from single apheresis donation (APCs). Materials and Methods: For each product type, 6 double-platelet concentrates were prepared and divided into 2 units; one was treated with RF-PI and the other by AM-PI. In vitro platelet-quality parameters were tested pre- and post-PI, at days 5 and 7. Results: Treatment and storage lesions were observed in PPCs and APCs with both PI methods. We found a higher rate of lactate increase and glucose depletion, suggesting a stronger stimulation of the glycolytic pathway, a higher Annexin V binding, and a loss of swirling in the RF-PI-treated units from day 5. The platelet loss was significantly higher in the AM-PI compared with the RF-PI units. Conclusions: Results suggest that RF-PI treatment has a higher deleterious impact on in vitro platelet quality compared to AM-PI, but we observed higher loss of platelets with AM-PI due to the post-illumination amotosalen adsorption step. If 7-day storage is needed, it can only be achieved with AM-PI, based on our quality criteria. |
| first_indexed | 2024-03-09T13:03:10Z |
| format | Article |
| id | doaj.art-cc3ad1ecc98d4f7fada9729dca5f680f |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T13:03:10Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-cc3ad1ecc98d4f7fada9729dca5f680f2023-11-30T21:52:44ZengMDPI AGPathogens2076-08172022-03-0111334310.3390/pathogens11030343In Vitro Comparative Study of Platelets Treated with Two Pathogen-Inactivation Methods to Extend Shelf Life to 7 DaysNicolas Malvaux0Fanette Defraigne1Styliani Bartziali2Camille Bellora3Kathleen Mommaerts4Fay Betsou5Anne Schuhmacher6Red Cross of Luxemburg, Boulevard Joseph II, 40, L-1840 Luxembourg, LuxembourgRed Cross of Luxemburg, Boulevard Joseph II, 40, L-1840 Luxembourg, LuxembourgRed Cross of Luxemburg, Boulevard Joseph II, 40, L-1840 Luxembourg, LuxembourgIntegrated Biobank of Luxembourg, 1 rue Louis Rech, L-3555 Dudelange, LuxembourgIntegrated Biobank of Luxembourg, 1 rue Louis Rech, L-3555 Dudelange, LuxembourgIntegrated Biobank of Luxembourg, 1 rue Louis Rech, L-3555 Dudelange, LuxembourgRed Cross of Luxemburg, Boulevard Joseph II, 40, L-1840 Luxembourg, LuxembourgBackground and Objectives: Since 2015, platelet products have been pathogen-inactivated (PI) at the Luxemburgish Red Cross (LRC) using Riboflavin and UV light (RF-PI). As the LRC should respond to hospital needs at any time, platelet production exceeds the demand, generating a discard rate of 18%. To reduce this, we consider the extension of storage time from 5 to 7 days. This study’s objective was to evaluate the in vitro 7-day platelet-storage quality, comparing two PI technologies, RF-PI and amotosalen/UVA light (AM-PI), for platelet pools from whole-blood donations (PPCs) and apheresis platelets collected from single apheresis donation (APCs). Materials and Methods: For each product type, 6 double-platelet concentrates were prepared and divided into 2 units; one was treated with RF-PI and the other by AM-PI. In vitro platelet-quality parameters were tested pre- and post-PI, at days 5 and 7. Results: Treatment and storage lesions were observed in PPCs and APCs with both PI methods. We found a higher rate of lactate increase and glucose depletion, suggesting a stronger stimulation of the glycolytic pathway, a higher Annexin V binding, and a loss of swirling in the RF-PI-treated units from day 5. The platelet loss was significantly higher in the AM-PI compared with the RF-PI units. Conclusions: Results suggest that RF-PI treatment has a higher deleterious impact on in vitro platelet quality compared to AM-PI, but we observed higher loss of platelets with AM-PI due to the post-illumination amotosalen adsorption step. If 7-day storage is needed, it can only be achieved with AM-PI, based on our quality criteria.https://www.mdpi.com/2076-0817/11/3/3437-day platelet storagepathogen inactivationplatelet quality |
| spellingShingle | Nicolas Malvaux Fanette Defraigne Styliani Bartziali Camille Bellora Kathleen Mommaerts Fay Betsou Anne Schuhmacher In Vitro Comparative Study of Platelets Treated with Two Pathogen-Inactivation Methods to Extend Shelf Life to 7 Days Pathogens 7-day platelet storage pathogen inactivation platelet quality |
| title | In Vitro Comparative Study of Platelets Treated with Two Pathogen-Inactivation Methods to Extend Shelf Life to 7 Days |
| title_full | In Vitro Comparative Study of Platelets Treated with Two Pathogen-Inactivation Methods to Extend Shelf Life to 7 Days |
| title_fullStr | In Vitro Comparative Study of Platelets Treated with Two Pathogen-Inactivation Methods to Extend Shelf Life to 7 Days |
| title_full_unstemmed | In Vitro Comparative Study of Platelets Treated with Two Pathogen-Inactivation Methods to Extend Shelf Life to 7 Days |
| title_short | In Vitro Comparative Study of Platelets Treated with Two Pathogen-Inactivation Methods to Extend Shelf Life to 7 Days |
| title_sort | in vitro comparative study of platelets treated with two pathogen inactivation methods to extend shelf life to 7 days |
| topic | 7-day platelet storage pathogen inactivation platelet quality |
| url | https://www.mdpi.com/2076-0817/11/3/343 |
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