Confinement to Organelle-Associated Inclusion Structures Mediates Asymmetric Inheritance of Aggregated Protein in Budding Yeast
The division of the S. cerevisiae budding yeast, which produces one mother cell and one daughter cell, is asymmetric with respect to aging. Remarkably, the asymmetry of yeast aging coincides with asymmetric inheritance of damaged and aggregated proteins by the mother cell. Here, we show that misfold...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2012-10-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124712002641 |
| _version_ | 1818114324766392320 |
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| author | Rachel Spokoini Ofer Moldavski Yaakov Nahmias Jeremy L. England Maya Schuldiner Daniel Kaganovich |
| author_facet | Rachel Spokoini Ofer Moldavski Yaakov Nahmias Jeremy L. England Maya Schuldiner Daniel Kaganovich |
| author_sort | Rachel Spokoini |
| collection | DOAJ |
| description | The division of the S. cerevisiae budding yeast, which produces one mother cell and one daughter cell, is asymmetric with respect to aging. Remarkably, the asymmetry of yeast aging coincides with asymmetric inheritance of damaged and aggregated proteins by the mother cell. Here, we show that misfolded proteins are retained in the mother cell by being sequestered in juxtanuclear quality control compartment (JUNQ) and insoluble protein deposit (IPOD) inclusions, which are attached to organelles. Upon exposure to stress, misfolded proteins accumulate in stress foci that must be disaggregated by Hsp104 in order to be degraded or processed to JUNQ and IPOD. Cells that fail to deliver aggregates to an inclusion pass on aggregates to subsequent generations. |
| first_indexed | 2024-12-11T03:48:55Z |
| format | Article |
| id | doaj.art-cc3bb23f3d3c4a43b8b553e093aaf12d |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-11T03:48:55Z |
| publishDate | 2012-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-cc3bb23f3d3c4a43b8b553e093aaf12d2022-12-22T01:21:57ZengElsevierCell Reports2211-12472012-10-012473874710.1016/j.celrep.2012.08.024Confinement to Organelle-Associated Inclusion Structures Mediates Asymmetric Inheritance of Aggregated Protein in Budding YeastRachel Spokoini0Ofer Moldavski1Yaakov Nahmias2Jeremy L. England3Maya Schuldiner4Daniel Kaganovich5Department of Cell and Developmental Biology, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, IsraelDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, IsraelDepartment of Cell and Developmental Biology, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, IsraelDepartment of Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, USADepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, IsraelDepartment of Cell and Developmental Biology, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, IsraelThe division of the S. cerevisiae budding yeast, which produces one mother cell and one daughter cell, is asymmetric with respect to aging. Remarkably, the asymmetry of yeast aging coincides with asymmetric inheritance of damaged and aggregated proteins by the mother cell. Here, we show that misfolded proteins are retained in the mother cell by being sequestered in juxtanuclear quality control compartment (JUNQ) and insoluble protein deposit (IPOD) inclusions, which are attached to organelles. Upon exposure to stress, misfolded proteins accumulate in stress foci that must be disaggregated by Hsp104 in order to be degraded or processed to JUNQ and IPOD. Cells that fail to deliver aggregates to an inclusion pass on aggregates to subsequent generations.http://www.sciencedirect.com/science/article/pii/S2211124712002641 |
| spellingShingle | Rachel Spokoini Ofer Moldavski Yaakov Nahmias Jeremy L. England Maya Schuldiner Daniel Kaganovich Confinement to Organelle-Associated Inclusion Structures Mediates Asymmetric Inheritance of Aggregated Protein in Budding Yeast Cell Reports |
| title | Confinement to Organelle-Associated Inclusion Structures Mediates Asymmetric Inheritance of Aggregated Protein in Budding Yeast |
| title_full | Confinement to Organelle-Associated Inclusion Structures Mediates Asymmetric Inheritance of Aggregated Protein in Budding Yeast |
| title_fullStr | Confinement to Organelle-Associated Inclusion Structures Mediates Asymmetric Inheritance of Aggregated Protein in Budding Yeast |
| title_full_unstemmed | Confinement to Organelle-Associated Inclusion Structures Mediates Asymmetric Inheritance of Aggregated Protein in Budding Yeast |
| title_short | Confinement to Organelle-Associated Inclusion Structures Mediates Asymmetric Inheritance of Aggregated Protein in Budding Yeast |
| title_sort | confinement to organelle associated inclusion structures mediates asymmetric inheritance of aggregated protein in budding yeast |
| url | http://www.sciencedirect.com/science/article/pii/S2211124712002641 |
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