Effects of Butterfly Pea Extracts on Phagocytic Activity of Blood Polymorphonuclear Leukocytes and Muscular Lipid Peroxidation in Rabbits
Sixteen 35-day-old male crossbred rabbits (New Zealand white × Thai native breed) with an initial weight of 484 ± 11.3 g were randomly divided into two groups of eight, constituting control and treatment groups. The treatment group was orally administered a crude extract of butterfly pea (<i>C...
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MDPI AG
2024-03-01
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| author | Attawit Kovitvadhi Laura Gasco Ivo Zoccarato Theera Rukkwamsuk |
| author_facet | Attawit Kovitvadhi Laura Gasco Ivo Zoccarato Theera Rukkwamsuk |
| author_sort | Attawit Kovitvadhi |
| collection | DOAJ |
| description | Sixteen 35-day-old male crossbred rabbits (New Zealand white × Thai native breed) with an initial weight of 484 ± 11.3 g were randomly divided into two groups of eight, constituting control and treatment groups. The treatment group was orally administered a crude extract of butterfly pea (<i>Clitoria ternatea</i> L.) at 0.5 g/kg body weight from weaning (at 35 days) to slaughter (at 90 days). The effects on the phagocytic activity of blood polymorphonuclear leukocytes, serum biochemistry, meat quality, muscular lipid peroxidation, the apparent digestibility of dry matter and nutrients, and gut histology were studied. The results revealed that the phagocytic function of circulating leukocytes (75 and 90 days) and alveolar macrophages (90 days) did not differ between the two groups. At slaughter, treated rabbits had lower blood urea nitrogen concentrations and higher liver weight than control rabbits (<i>p</i> < 0.05). After chilling at 4 °C for 24 h, a lower meat pH and the alteration of meat color (brighter, less yellow, lower hue angle, and decreased color saturation) were observed in the treated group (<i>p</i> < 0.05). Furthermore, lipid peroxidation (measured at 3, 5, and 7 storage days) in the meat of treated rabbits was lower than in controls (<i>p</i> < 0.05). The apparent digestibility of organic matter and ether extract (analyzed at 46 days for 4 days) was improved in the treated group (<i>p</i> < 0.05), whereas gut histology was unaffected. In conclusion, butterfly pea extract supplementation did not affect phagocytic function but led to a modification in meat color, delayed lipid peroxidation, and improved digestibility. |
| first_indexed | 2024-04-24T18:38:08Z |
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| language | English |
| last_indexed | 2024-04-24T18:38:08Z |
| publishDate | 2024-03-01 |
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| series | Animals |
| spelling | doaj.art-cc3c37a960394aba8002afbe4c950e122024-03-27T13:17:57ZengMDPI AGAnimals2076-26152024-03-0114695810.3390/ani14060958Effects of Butterfly Pea Extracts on Phagocytic Activity of Blood Polymorphonuclear Leukocytes and Muscular Lipid Peroxidation in RabbitsAttawit Kovitvadhi0Laura Gasco1Ivo Zoccarato2Theera Rukkwamsuk3Department of Agriculture, Forest, and Food Sciences, University of Turin, Largo P. Braccini 2, 10095 Turin, ItalyDepartment of Agriculture, Forest, and Food Sciences, University of Turin, Largo P. Braccini 2, 10095 Turin, ItalyDepartment of Agriculture, Forest, and Food Sciences, University of Turin, Largo P. Braccini 2, 10095 Turin, ItalyDepartment of Large Animal and Wildlife Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, ThailandSixteen 35-day-old male crossbred rabbits (New Zealand white × Thai native breed) with an initial weight of 484 ± 11.3 g were randomly divided into two groups of eight, constituting control and treatment groups. The treatment group was orally administered a crude extract of butterfly pea (<i>Clitoria ternatea</i> L.) at 0.5 g/kg body weight from weaning (at 35 days) to slaughter (at 90 days). The effects on the phagocytic activity of blood polymorphonuclear leukocytes, serum biochemistry, meat quality, muscular lipid peroxidation, the apparent digestibility of dry matter and nutrients, and gut histology were studied. The results revealed that the phagocytic function of circulating leukocytes (75 and 90 days) and alveolar macrophages (90 days) did not differ between the two groups. At slaughter, treated rabbits had lower blood urea nitrogen concentrations and higher liver weight than control rabbits (<i>p</i> < 0.05). After chilling at 4 °C for 24 h, a lower meat pH and the alteration of meat color (brighter, less yellow, lower hue angle, and decreased color saturation) were observed in the treated group (<i>p</i> < 0.05). Furthermore, lipid peroxidation (measured at 3, 5, and 7 storage days) in the meat of treated rabbits was lower than in controls (<i>p</i> < 0.05). The apparent digestibility of organic matter and ether extract (analyzed at 46 days for 4 days) was improved in the treated group (<i>p</i> < 0.05), whereas gut histology was unaffected. In conclusion, butterfly pea extract supplementation did not affect phagocytic function but led to a modification in meat color, delayed lipid peroxidation, and improved digestibility.https://www.mdpi.com/2076-2615/14/6/958antioxidant<i>Clitoria ternatea</i>digestibilitylipid peroxidationphagocytosis |
| spellingShingle | Attawit Kovitvadhi Laura Gasco Ivo Zoccarato Theera Rukkwamsuk Effects of Butterfly Pea Extracts on Phagocytic Activity of Blood Polymorphonuclear Leukocytes and Muscular Lipid Peroxidation in Rabbits Animals antioxidant <i>Clitoria ternatea</i> digestibility lipid peroxidation phagocytosis |
| title | Effects of Butterfly Pea Extracts on Phagocytic Activity of Blood Polymorphonuclear Leukocytes and Muscular Lipid Peroxidation in Rabbits |
| title_full | Effects of Butterfly Pea Extracts on Phagocytic Activity of Blood Polymorphonuclear Leukocytes and Muscular Lipid Peroxidation in Rabbits |
| title_fullStr | Effects of Butterfly Pea Extracts on Phagocytic Activity of Blood Polymorphonuclear Leukocytes and Muscular Lipid Peroxidation in Rabbits |
| title_full_unstemmed | Effects of Butterfly Pea Extracts on Phagocytic Activity of Blood Polymorphonuclear Leukocytes and Muscular Lipid Peroxidation in Rabbits |
| title_short | Effects of Butterfly Pea Extracts on Phagocytic Activity of Blood Polymorphonuclear Leukocytes and Muscular Lipid Peroxidation in Rabbits |
| title_sort | effects of butterfly pea extracts on phagocytic activity of blood polymorphonuclear leukocytes and muscular lipid peroxidation in rabbits |
| topic | antioxidant <i>Clitoria ternatea</i> digestibility lipid peroxidation phagocytosis |
| url | https://www.mdpi.com/2076-2615/14/6/958 |
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